Microbiome Research Core

微生物组研究核心

• 批准号: 10207675
• 负责人: Brandon Briggs
• 金额: $ 16.35万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-16 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10207675
• 关键词: Active Learning; Alaska; Algorithms; American; Anaerobic Bacteria; Awareness; Basic Science; Bioinformatics; Bioreactors; Cells; Centers of Research Excellence; Clinical; Collaborations; Computer software; Computers; Consultations; Cytolysis; Data Analyses; Discipline; Ecology; Elements; Ensure; Equipment; Equipment and Supplies; Event; Experimental Designs; Faculty; General Population; Genome; Goals; Health; Hibernation; Incubators; Infrastructure; Laboratory culture; Lead; Link; Metabolic; Metabolic Pathway; Metabolism; Microbe; Microbiology; Molecular; Molecular Analysis; Nutrient; Phase; Play; Procedures; Process; Research; Research Personnel; Research Project Grants; Research Support; Research Training; Resources; Role; Services; Societies; Students; Symbiosis; System; Taxonomy; Technology; Training; Translational Research; Universities; base; bioinformatics tool; computerized data processing; host microbiome; microbial; microbial community; microbiome; microbiome research; nanopore; novel; outreach; programs; research data dissemination; sample collection; symposium; tool

Project Summary - Microbiome Core Characterization of microbial communities and isolates is a key element necessary to achieve the scientific objectives of the proposed Center in Transformative Research in Metabolism. The Microbiome Core will provide a unique service dedicated to microbiome research and training. The goal of the Core is to support a comprehensive pipeline for characterization of microbial communities and isolates, which when characterized will generate testable hypotheses explaining host-microbiome symbiosis. We will achieve this goal by accomplishing the following Specific Aims (SA). SA1. Provide and maintain state-of-the-art technologies and procedures for a high throughput pipeline that characterizes microbial communities and isolates. SA2. Provide expertise in microbiome research, from experimental design and sample collection to data analysis and bioinformatics. SA3. Support and grow microbiome research and dissemination through expert consultation, and by engaging in outreach events with COBRE investigators and the general public. The novelty of this proposed core is to combine multiple modules that researchers can plug into at any point along the pipeline or access the full pipeline to characterize either isolates or microbial communities. The Microbiome Core is an essential component of the Research Projects supported by the Phase 1 center that focus on basic research into microbe-host symbiosis in metabolism. The Core provides the expertise, equipment, and software required for organismal, molecular, and bioinformatic analysis. Key equipment maintained by the Core includes a microbial bioreactor that allows users to set up experimental conditions similar to host guts and perturb the system to identify key microbial processes. An Illumina MiSeq and Nanopore MinION will provide the sequencing power to analyze the molecular underpinnings. In collaboration with the INBRE Bioinformatics Core, the Microbiome Core will provide expertise on bioinformatic algorithms needed for microbiome research.

项目摘要 - 微生物组核心 微生物群落和分离株的表征是实现科学的关键要素 代谢变革性研究中心提议的中心的目标。微生物组核心将 提供专门用于微生物组研究和培训的独特服务。核心的目的是支持 用于表征微生物群落和分离株的综合管道，在表征时 将产生可检验的假设，解释宿主 - 微生物组共生。我们将通过 完成以下特定目标（SA）。 SA1。提供并维护最先进的技术和 高吞吐管道的程序，其特征是微生物群落和分离株的特征。 SA2。提供 微生物组研究的专业知识，从实验设计和样本收集到数据分析和 生物信息学。 SA3。通过专家咨询来支持和发展微生物组研究和传播 并通过与山羊调查员和公众进行外展活动。这个新奇 建议的核心是将研究人员在管道或管道中的任何时候插入的多个模块组合 访问完整的管道以表征分离株或微生物群落。微生物组核心是 第1阶段中心支持的研究项目的基本组成部分，专注于基础研究 进入代谢中的微生物宿主共生。核心提供了所需的专业知识，设备和软件 用于生物，分子和生物信息学分析。核心维护的关键设备包括 微生物生物反应器，允许用户设置类似于主机肠和扰动的实验条件 确定关键微生物过程的系统。 Illumina Miseq和Nanobore Minion将提供 测序能力分析分子基础。与近亲生物信息学合作 核心，微生物组核心将提供有关微生物组研究所需的生物信息学算法的专业知识。