Core D: Cardiometabolic Phenotyping Core

核心 D：心脏代谢表型核心

基本信息

批准号：
10206253
负责人：
Jonathan Mark Brown
金额：
$ 25.76万
依托单位：
CLEVELAND CLINIC LERNER COM-CWRU
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10206253
关键词：
Adipose tissue Animal Model Animal Sources Animals Area Atherosclerosis Bile Acids Bioinformatics Blood Platelets Cardiometabolic Disease Cells Clinic Clinical Collaborations Controlled Environment Coupled Development Diet Disease Energy Metabolism Engineering Ensure Formulation Genotype Germ Germ-Free Gnotobiotic Goals Human Individual Insulin Investigation Lesion Lipids Lipoproteins Lyase Mass Spectrum Analysis Metabolic Metabolism Metagenomics Methodology Methods Modeling Mus Obesity Pathogenesis Pathway interactions Performance Phenotype Protocols documentation Reagent Research Personnel Research Project Grants Role Sampling Services Standardization Sterilization Study models Sulfate System Technical Expertise Thrombosis Tissues Tracer Training Support Transplantation Universities Work bile acid metabolism blood glucose regulation cardiometabolism chemical synthesis data sharing design disease phenotype gut microbes gut microbiota in vivo inhibitor/antagonist insulin sensitivity investigator training metabolomics microbial microbial community microbiome analysis mouse model novel strategies pre-clinical pressure programs seal small molecule stable isotope trait transcriptome sequencing trimethylamine

项目摘要

Cardiometabolic Phenotyping Core (Core D). ABSTRACT: The overall goal of the Cardiometabolic Disease Phenotyping Core (Core D) is to provide centralized expertise for performance of state-of-the-art animal model studies relevant to Cardiometabolic Diseases in support of Projects within this Program. This core is necessary to establish consistency in mouse phenotyping studies performed across all three Projects, and will ensure seamless data sharing and comparison of how each of the different gut microbial metabolite pathways under investigation impact cardiometabolic disease in mice. All projects in this PPG propose to examine the impact of select gut microbe-derived metabolites on host cardiometbolic disease phenotypes including atherosclerosis, thrombosis, adiposity, insulin/glucose homeostasis, and altered lipid/bile acid metabolism. Each of these phenotypes requires independent rigorous methods to be carried out by well-trained investigators in each area. The Cardiometabolic Disease Phenotyping Core (Core D) will provide technical expertise in a centralized mouse phenotyping service that will collaboratively interface with all other research projects and cores to examine the effect of altering gut microbial metabolites on cardiometabolic disease phenotypes.

心脏代谢表型核心（核心 D）。抽象的：心脏代谢疾病表型核心（核心 D）的总体目标是提供集中的专业知识进行与心脏代谢疾病相关的最先进的动物模型研究，以支持本计划内的项目。该核心对于建立小鼠表型研究的一致性是必要的在所有三个项目中执行，并将确保无缝数据共享和每个项目的比较正在研究的不同肠道微生物代谢途径对小鼠心脏代谢疾病的影响。全部该 PPG 中的项目建议研究特定肠道微生物衍生代谢物对宿主的影响心脏代谢疾病表型，包括动脉粥样硬化、血栓形成、肥胖、胰岛素/血糖体内平衡和改变脂质/胆汁酸代谢。这些表型中的每一个都需要独立严格的由每个领域训练有素的调查人员执行的方法。心脏代谢疾病表型分析核心（核心 D）将提供集中式小鼠表型分析服务的技术专业知识，该服务将与所有其他研究项目和核心协作接口，以检查改变肠道的效果微生物代谢物对心脏代谢疾病表型的影响。