Redox Metabolism Shared Resource Facility

氧化还原代谢共享资源设施

基本信息

批准号：
10204889
负责人：
D. Allan Butterfield
金额：
$ 15.75万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-08 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10204889
关键词：
Affect Appalachian Region Area Australia Basic Science Bioenergetics Biological Biological Assay Biology Cancer Biology Cancer Burden Cancer Center Cancer Center Support Grant Cancer Control Research Catchment Area Cell Respiration Cells Centers of Research Excellence Chemotherapy-Oncologic Procedure Clinical Comprehensive Cancer Center Consultations Development Education Educational workshop Equipment Europe Experimental Designs Faculty Free Radicals Funding Future Genomics Genus Hippocampus Goals Immunotherapy Incidence Institution International Investments Kentucky Knowledge Leadership Malignant Neoplasms Mass Spectrum Analysis Measurement Mentors Metabolic Metabolic Pathway Metabolism Methodology Methods Mission Mitochondria Molecular Morphologic artifacts NCI Center for Cancer Research National Cancer Institute Neoplasm Metastasis Normal tissue morphology Nuclear Magnetic Resonance Obesity Oxidation-Reduction Oxidative Stress Oxides Patients Peer Review Phase Population Post-Translational Protein Processing Preparation Principal Investigator Protein Array Proteins Proteomics Research Research Personnel Resource Sharing Resources Role Sampling Science Services Signal Transduction Strategic Planning System Techniques Technology Tissues Training Training and Education Translational Research United States United States National Institutes of Health Universities bench to bedside biological systems biomarker discovery biomarker validation cancer prevention cancer therapy data integration differential expression high risk population improved innovation insight interest lens member metabolomics metabolomics resource mortality nitrosative stress oxidative damage precision medicine programs recruit response service utilization side effect stable isotope tissue injury tumor metabolism

项目摘要

PROJECT SUMMARY/ABSTRACT The Redox Metabolism Shared Resource Facility (RM SRF) is a cancer-center managed SRF that provides critical services for University of Kentucky (UK) Markey Cancer Center (MCC) Research Programs. Notably, the RM SRF is one of only a small number of facilities directly associated with a National Cancer Institute-designated Cancer Center that provides expertise and analyses in oxidative stress, cellular energetics, metabolomics and proteomics in cancer and cancer biology. Over the last 4 years of CCSG funding, the RM SRF has successfully demonstrated its high value-added role for MCC investigators doing basic science, clinical and cancer prevention and control research in the MCC's Kentucky catchment area. There is increasing evidence that oxidative damage, with sequelae of altered cellular energetics and metabolism as well as protein alterations, underlie the initiation, promotion, invasion, metastasis and treatment of cancers. Moreover, oxidative stress is implicated in the side effects of normal tissue injury following cancer therapy. As such, the 4 basic services provided by the RM SRF are: 1) analysis of markers of oxidative and nitrosative stress; 2) molecular biological manipulation of biological systems to investigate redox signals, including measurements of mitochondrial function by Seahorse technology; 3) proteomics identification of differentially expressed, differentially oxidized or differentially covalently modified proteins in various cancer- related systems; and 4) profiling and Stable Isotope Resolved Metabolomics of cancer tissue and cells, a service that began in 2015 following a $6M UK investment in nuclear magnetic resonance and mass spectrometry equipment. Key improvements since initial CCSG funding include expanding services and scope toward metabolomics as well as increasing staff. This SRF is directed by Dr. D. Allan Butterfield (GEM) with Dr. Haining Zhu (GEM), as Associate Director. With the exciting addition of metabolomics analyses to the SRF, Dr. Richard Higashi (GEM), an expert in the assessment of metabolites and metabolic pathways, has been added as Associate Director of RM SRF for metabolomics. Fifty-four MCC members used RM SRF services since MCC funding began, a 43% increase from year 1 to year 4 of CCSG funding. Of these, 39 were members with peer-reviewed funding. RM SRF leadership and staff actively participate in the development of new applications and methodologies for a better understanding of the roles of oxidative stress and metabolism in cancer and cancer chemotherapy and provide technical assistance with experimental design and interpretation of results for MCC principal investigators. RM SRF technical staff have knowledge of the latest and most current sample handling and preparation methods so that reliable, precise and artifact-free assay results can be obtained. The RM SRF facilitates research with the other MCC SRFs to improve the analytical power of various cutting-edge technologies for understanding of the enormous cancer burden in Kentucky.

项目摘要/摘要氧化还原代谢共享资源设施（RM SRF）是一个癌症中心的托管SRF，为肯塔基大学（英国）Markey Cancer Center（MCC）研究计划提供关键服务。值得注意的是，RM SRF是与国家癌症直接相关的仅有的少数设施之一研究所指定的癌症中心，提供氧化应激，细胞的专业知识和分析癌症和癌症生物学中的能量，代谢组学和蛋白质组学。在CCSG的最后4年 RM SRF的资助已成功证明了其在MCC研究人员中的高增值角色 MCC肯塔基州集水区的基础科学，临床和癌症预防和控制研究。越来越多的证据表明，氧化损伤，细胞能量改变的后遗症和代谢以及蛋白质改变，启动，促进，入侵，转移和治疗是基础癌症。此外，氧化应激与癌症后正常组织损伤的副作用有关治疗。因此，RM SRF提供的4种基本服务是：1）分析氧化标记和亚硝化应激； 2）生物系统的分子生物学操纵，以研究氧化还原信号，包括通过海马技术测量线粒体功能； 3）蛋白质组学的识别在各种癌症中以差异表达，差异化氧化或差异化修饰的蛋白相关系统； 4）分析和稳定的同位素分辨癌组织和细胞的代谢组学，A 在英国对核磁共振和质量进行600万美元的投资之后，该服务于2015年开始光谱设备。自初始CCSG资金以来的主要改进包括扩大服务和范围迈向代谢组学以及增加员工。该SRF由D. Allan Butterfield博士（GEM）与博士执导。 Haining Zhu（宝石），副主任。随着对SRF的激动人心的代谢组学分析，博士 Richard Higashi（GEM）是代谢物和代谢途径评估的专家作为代谢组学RM SRF的副主任。五十四名MCC成员使用RM SRF服务以来 MCC的资金开始了，从CCSG资助的1年级到4年增加了43％。其中39位成员同行评审的资金。 RM SRF领导力和员工积极参与新的发展应用和方法论，以更好地理解氧化应激和代谢在癌症和癌症化疗，并在实验设计和解释方面提供技术援助 MCC首席研究人员的结果。 RM SRF技术人员了解最新，最多当前的样品处理和制备方法，因此可靠，精确和无伪影的测定结果可以获得。 RM SRF促进了其他MCC SRF的研究，以提高各种尖端技术，以了解肯塔基州巨大的癌症负担。