PROJECT 7. Free Radical

项目 7. 自由基

• 批准号: 8740616
• 负责人: D. Allan Butterfield
• 金额: $ 6.73万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-08 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8740616
• 关键词: Address; Adverse effects; Advisory Committees; Area; Bioenergetics; Biological; Biological Assay; Biology; Buffers; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cancer Control; Cancer Etiology; Cellular biology; Chemotherapy-Oncologic Procedure; Clinical Research; Clinical Trials; Detection; Development; Electron Spin Resonance Spectroscopy; Etiology; Evaluation; Free Radicals; Freezing; Genus Hippocampus; Grant; Injury; Investigation; Ions; Kentucky; Lipids; Malignant Neoplasms; Manuscripts; Measurement; Measures; Mediating; Membrane; Metabolism; Metals; Methodology; Methods; Mitochondria; Modeling; Modification; Molecular; Morphologic artifacts; Neoplasm Metastasis; Normal tissue morphology; Nucleic Acids; Oxidation-Reduction; Oxidative Stress; Preparation; Proteins; Proteomics; Reagent; Research; Research Personnel; Resource Sharing; Resources; Role; Sampling; Services; Signal Transduction; Strategic Planning; System; Techniques; Technology; Therapeutic; Training; Universities; biological systems; cancer cell; cancer prevention; cancer therapy; carcinogenesis; cell injury; chemotherapy; drug discovery; injury and repair; insight; instrumentation; interest; member; mitochondrial dysfunction; nitrosative stress; organizational structure; oxidative damage; pre-clinical; programs; sample fixation; tumor progression

There is increasing evidence that oxidative damage and free radicals, with sequelae of altered cellular energetics and protein alterations, are important in the initiation, promotion, invasion, metastasis, and treatment of cancers. Moreover, oxidative damage and free radicals also are implicated in the side effects of normal tissue injury following cancer therapy, including chemotherapy, radiafion, and biological modifiers. The Free Radical Biology in Cancer Shared Resource Facility (FRBC SRF) was established to provide expertise in and analyses of free radicals, cellular energetics, and reactive species, as well as proteomics in cancer and cancer biology for Markey Cancer Center (MCC) investigators who perform basic, pre-clinical, and clinical research. The four basic services provided by the FRBC SRF are: 1) Analysis of markers of oxidative and nitrosative stress; 2) Molecular biological manipulation of biological systems with which to investigate redox signals, including measurements of mitochondrial function by Seahorse technology; 3) Proteomics identification of differentially expressed, differentially oxidized, or differentially covalently modified proteins in various cancer-related systems; 4) Electron paramagnetic resonance (EPR) detection of free radicals. Development of new applications and methodology for better understanding of the roles of free radicals in cancer and cancer chemotherapy and technical assistance with grant and manuscript preparation by MCC investigators will also be provided. Included in these functions of the considerable expertise of the FRBC SRF Technical Advisory Committee to educate MCC investigators on proper sample handling and preparation methods so that reliable, precise, and artifact-free assay results are obtained. Multiple investigators from all four research programs of the MCC (Redox Injury and Repair [RR]; Cancer Cell Biology and Signaling [CS]; Cancer Prevention and Control [CP]; and Drug Discovery, Delivery and Translational Therapeutics [DT]) have used one or more services of the FRBC SRF. Indeed, even as a relatively new shared resource, the FRBC SRF still had usage by 26% of MCC investigators.

有越来越多的证据表明，氧化损伤和自由基具有改变细胞能量和蛋白质改变的后遗症，在起始，促进，侵袭，转移和癌症治疗中很重要。此外，氧化损伤和自由基也与癌症治疗后正常组织损伤的副作用有关，包括化学疗法，疗程和生物修饰剂。建立了癌症共享资源设施中的自由基生物学（FRBC SRF），以提供自由基，细胞能量和反应性物种的专业知识和分析，以及Markey Cancer Center（MCC）的癌症和癌症生物学中的蛋白质组学研究者（MCC）研究人员进行基本，临床前和临床研究。 FRBC SRF提供的四个基本服务是：1）分析氧化和亚硝化应激标记； 2）生物系统的分子生物操纵，以研究氧化还原信号，包括通过海马技术测量线粒体功能； 3）在各种癌症相关系统中，蛋白质组学鉴定差异表达，差异化或差异化修饰的蛋白质； 4）电子顺磁共振（EPR）检测自由基。还将提供新的应用和方法论，以更好地理解自由基在癌症和癌症化学疗法中的作用，以及MCC研究人员的赠款和手稿准备的技术援助。包括FRBC SRF技术咨询委员会的大量专业知识的这些职能中包括，以教育MCC调查人员有关适当的样品处理和准备方法，以便获得可靠，精确和无伪影的无分析结果。来自MCC的所有四个研究计划的多个研究人员（氧化还原损伤和修复[RR]；癌细胞生物学和信号[CS]；预防和对照[CP]；以及药物发现，分娩和转化治疗[DT]） FRBC SRF的一项或多项服务。确实，即使是一种相对较新的共享资源，FRBC SRF仍然使用了26％的MCC调查人员使用。