Noninvasive Pesticide Biomonitoring Using Sweat Patches

使用汗贴进行非侵入性农药生物监测

• 批准号: 10371353
• 负责人: JOSEPH G. GRZYWACZ
• 金额: $ 23.1万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10371353
• 关键词: Noninvasive; Pesticide; Biomonitoring; Using; Sweat

PROJECT SUMMARY Chronic low-dose exposure to agricultural pesticides is widely accepted as a fundamental health threat to farmworkers. Unfortunately, delineating sources of variation in pesticide exposure among farmworkers and the long-term consequences of pesticide exposure is hampered by measurement challenges, particularly the absence of an easy, nonintrusive strategy for pesticide biomonitoring. Sweat patches offer a compelling alternative for assessing exposure to pesticides in field studies. Evidence indicates that pesticide metabolites are found in sweat, and sweat patches are being used routinely now in drug monitoring studies. The goal of this interdisciplinary study is to determine the feasibility of using sweat patches for biomonitoring pesticide exposure among Latino farmworkers. We will test the hypothesis that sweat patch sampling will allow for sensitive and consistent measurements with a high rate of compliance. The aims of this feasibility study are to: 1) develop and assess the accuracy and precision of laboratory techniques used to measure pesticides and their metabolites extracted from sweat patches and 2) determine the levels and variance in concentrations of pesticides and metabolites obtained from sweat to those obtained from urine. A secondary aim is to document challenges and problems experienced by farmworkers in using (e.g., opening, applying, wearing removing, and storing) the sweat patches. Advantages of sweat patches to traditional urine screening include the patch being noninvasive, showing increased compliance, and reducing financial cost for future research in this field. Our proposed work involves a combination of both laboratory and field procedures, and the subsequent integration will result in a highly translational project with substantial occupational health impact.

项目摘要 慢性低剂量暴露于农业农药被广泛认为是对健康的基本健康威胁 农场工人。不幸的是，描述了农民工和农药暴露差异的来源 衡量挑战会阻碍农药暴露的长期后果，尤其是 没有一种简单的，非感染的农药生物监测策略。汗水补丁提供了引人入胜的 评估现场研究中农药暴露的替代方法。证据表明农药代谢物 在汗水中发现，现在在药物监测研究中常规使用汗水。目标的目标 跨学科研究是为了确定使用汗水斑块进行生物监测的可行性 在拉丁裔农场工人中。我们将检验以下假设，即汗水斑块采样将允许敏感和 一致的测量率具有很高的合规性。这项可行性研究的目的是：1）发展和 评估用于测量农药及其代谢物的实验室技术的准确性和精度 从汗水斑块中提取和2）确定农药浓度的水平和差异 从汗水获得的代谢产物到从尿液中获得的代谢产物。次要目的是记录挑战和 农场工人在使用中遇到的问题（例如，开放，申请，佩戴和存储） 汗水补丁。传统尿液筛查的汗水斑块的优点包括无创的斑块， 表现出提高的合规性，并降低了该领域未来研究的财务成本。我们建议的工作 涉及实验室和现场程序的组合，随后的整合将导致 具有重大职业健康影响的高度转化项目。