Development Core

开发核心

• 批准号: 10200815
• 负责人: TIMOTHY RAYMOND FENNELL
• 金额: $ 33.23万
• 依托单位: RESEARCH TRIANGLE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-05 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10200815
• 关键词: Big Data Methods; Biological; Biological Markers; Chemical Exposure; Chemicals; Coupled; Detection; Development; Ensure; Environmental Exposure; Feces; Food; Gas Chromatography; Health; Human; Ingestion; Institution; Laboratories; Libraries; Liquid substance; Mass Fragmentography; Mass Spectrum Analysis; Measures; Metabolism; Methods; Natural Products; Pesticides; Pharmaceutical Preparations; Phenols; Plants; Plasma; Polychlorinated Biphenyls; Procedures; Protocols documentation; Reference Standards; Resolution; Resources; Saliva; Sampling; Schedule; Serum; Services; Signal Transduction; Source; Techniques; Testing; Tissues; Toxic effect; Toxicology; Translating; U-Series Cooperative Agreements; Urine; Work; base; biomarker development; data acquisition; drug of abuse; epidemiology study; experience; in vivo; liquid chromatography mass spectrometry; mass spectrometer; multiple reaction monitoring; novel strategies; polybrominated diphenyl ether; polyphenol; sample collection; working group

Project Summary/Abstract (Development Core) The Development Core (DC) and the Untargeted Analysis Resource Core (UARC) will collaborate, with the overall aims to increase the range of analytes or biological markers that are detected and identified that reflect environmental exposures. The DC will work with the Big Data Analytics of UARC to prioritize the sourcing of compounds. The DC will optimize extraction procedures for new matrices, in addition to the matrices already analyzed (e.g., urine, serum, plasma, feces, saliva and sweat), and will conduct analyses of biospecimens collected and stored under different conditions to inform best practices for epidemiology studies. They will establish an untargeted method using gas chromatography coupled to an orbitrap mass spectrometer (GC-Q- Exactive) to expand the services offered by the UARC. The DC will also use GC- and liquid chromatography (LC)-Mass spectrometry with Multiple Reaction Monitoring (MRMs) and MSn to develop specific broad spectrum panels for rapid quantitative analysis of analytes that are difficult to detect on the untargeted platforms. The DC will be directed by Dr. Timothy Fennell (MPI, RTI), who has extensive experience with chemical exposure, metabolite identification and toxicity, and exposure biomarker development.

项目总结/摘要（开发核心） 开发核心 (DC) 和非目标分析资源核心 (UARC) 将与 总体目标是增加检测和识别的分析物或生物标记的范围，以反映 环境暴露。 DC 将与 UARC 的大数据分析部门合作，优先考虑采购 化合物。除了已经存在的矩阵之外，DC 还将优化新矩阵的提取程序 进行分析（例如尿液、血清、血浆、粪便、唾液和汗液），并将对生物样本进行分析 在不同条件下收集和存储，为流行病学研究的最佳实践提供信息。他们会 使用气相色谱法与轨道阱质谱仪 (GC-Q- Exactive）以扩展 UARC 提供的服务。 DC 还将使用 GC 和液相色谱 (LC)-质谱联用多反应监测 (MRM) 和 MSn，以开发特异性广泛的 光谱板用于对非目标物上难以检测的分析物进行快速定量分析 平台。 DC 将由 Timothy Fennell 博士（MPI、RTI）领导，他在以下领域拥有丰富的经验： 化学品暴露、代谢物鉴定和毒性以及暴露生物标志物的开发。