Botanical Core

植物核心

• 批准号: 10200689
• 负责人: James E Simon
• 金额: $ 17.29万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10200689
• 关键词: Accounting; Adopted; Animals; Anthocyanins; Archives; Area; Behavioral; Biological; Biological Availability; Biometry; Blood; Botanical dietary supplements; Botanicals; COSY; Catechin; Clinical Pharmacology; Clinical Trials; Cognition; Cognitive; Communication; Coupled; Dietary Polyphenol; Dietary Supplementation; Drug or chemical Tissue Distribution; Ensure; Flavonoids; Flavonols; Fostering; Goals; Grapes; Human; Human Resources; Immunosuppression; Individual; Industrialization; Juice; Leadership; Life; Liquid Chromatography; Liquid substance; Mass Chromatography; Mass Spectrum Analysis; Methods; Mus; NOESY; Nature; Nuclear; Parents; Phase; Phenols; Plasma; Procedures; Protocols documentation; Quality Control; Quercetin; Research; Research Personnel; Research Project Grants; Resolution; Resveratrol; Sampling; Ships; Spectrum Analysis; Stilbenes; Stress; System; TOCSY; Techniques; Testing; Tissues; Validation; Work; brain tissue; design; epicatechin; experimental study; grape seed; in vivo; metabolic abnormality assessment; novel; nuclear Overhauser enhancement; phenolic acid; polyphenol; preservation; psychologic; quality assurance; quantum; repository; resilience; sample archive; screening; stability testing; stress related disorder; tandem mass spectrometry

Project Summary/Abstract The purpose of Research Core B is to support the overall effort of the ODS/NCCIH U19 Botanical Dietary Supplement Research Center (BDSRC) in two specific areas: (1) by centralizing the procurement, characterization, archiving, quality control and shelf-life/stability assessment of polyphenolic materials used by individual Research Projects; and (2) by centralizing bioanalytical support for all Center Research Projects and Cores. Our previous studies have demonstrated the remarkable efficacy of dietary supplementation from a combination of grape seed polyphenolic extract (GSPE), Concord grape juice (CGJ), and resveratrol (RSV) in promoting preservation of cognitive wellness and psychological resiliency under select stress conditions. We have also shown that specific metabolites extracted from these botanicals can be detected in blood and brain tissue. We can detect methylated and/or glucuronidated derivatives of the various classes of flavonoids (flavan-3-ols such as catechin and epicatechin; anthocyanins (cyanidin and malvidin); flavonols (quercetin); stilbenes (resveratrol)). Core B will be responsible for the identification and quantitation of the parent compounds and derived metabolites in animal and human biological fluids and tissues, using various analytical techniques including liquid chromatography (LC) coupled to different mass spectrometric (MS) techniques, e.g. high resolution mass spectrometry (HRMS) and triple quadrupole tandem mass spectrometry (QqQ-MS/MS), 1D nuclear magenetic resonance (NMR) techniques including 1H NMR and 13C NMR, and various 2D NMR techniques including 1H-1H TOCSY (total correlated spectroscopy), 1H-1H NOESY (nuclear Overhauser enhancement spectroscopy), gradient enhanced 1H-1H COSY (correlated spectroscopy), 1H-13C HSQC (heteronuclear single quantum coherence), and 1H-13C HMBC (heteronuclear multiple bond coherence), and LC-NMR as needed. In addition to these bioavailability and PK studies, Core B will also serve as an archived repository of all botanical materials, standards and polyphenolic compounds used throughout the Center, maintain tracking of all samples using a LIMS system, and perform quality control analyses and stability testing in support of this function. The complementary nature of the expertise of the individual investigators within Core B creates a strong synergistic team capable of supporting individual Research Projects and collaborative efforts focused on achieving U19 BDSRC objectives.

项目摘要/摘要 研究核心B的目的是支持ODS/NCCIH U19植物饮食的整体努力 在两个特定领域的补充研究中心（BDSRC）：（1）通过集中采购， 对多酚材料的表征，归档，质量控制和保质期/稳定性评估 个人研究项目； （2）通过集中对所有中心研究项目的生物分析支持和 内核。我们以前的研究表明，饮食补充的显着疗效 葡萄种子多酚提取物（GSPE），Concord Grape Juice（CGJ）和白藜芦醇（RSV）的组合 在某些压力条件下促进认知健康和心理弹性的保存。我们 还表明，可以在血液和脑中检测到从这些植物体中提取的特定代谢产物 组织。我们可以检测各种类黄酮的甲基化和/或葡萄糖醛酸苷衍生物 （Flavan-3-ols，例如儿茶素和表面环素；花青素（氰胺素和玛氏素）；黄酮醇（槲皮素）； Stilbenes（白藜芦醇））。核心B将负责父母的识别和定量 动物和人类生物液和组织中的化合物和衍生代谢物，使用各种分析 包括液相色谱（LC）在内的技术，耦合到不同的质谱（MS）技术，例如 高分辨率质谱法（HRM）和三极方形串联质谱法（QQQ-MS/MS）， 1D核神经共振（NMR）技术，包括1H NMR和13C NMR，以及各种2D NMR 包括1H-1H TOCSY（总相关光谱），1H-1H NOESY（核透射器）在内的技术 增强光谱法），梯度增强1H-1H舒适（相关光谱），1H-13C HSQC （异核单量子相干性）和1H-13C HMBC（异核多键相干性），并且 LC-NMR根据需要。除了这些生物利用度和PK研究外，核心B还将作为存档 整个中心使用的所有植物材料，标准和多酚化合物的存储库， 使用LIMS系统维护所有样品的跟踪，并执行质量控制分析和稳定性测试 支持此功能。个人调查人员专业知识的互补性质 核心B创建了一个强大的协同团队，能够支持各个研究项目和协作 旨在实现U19 BDSRC目标的努力。