[RNW] - Gamete Interactions in Caenorhabditis elegans

[RNW] - 秀丽隐杆线虫配子相互作用

• 批准号: 10187604
• 负责人: ANDREW W. SINGSON
• 金额: $ 32.28万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10187604
• 关键词: Address; Affect; Animals; Area; Biochemical; Biological; Biological Models; Biological Process; Biology; Caenorhabditis elegans; Cell Communication; Cell membrane; Cells; Collection; Complement; Contraceptive Agents; Coupled; Embryo; Epidermal Growth Factor; Equipment and supply inventories; Event; Fertility; Fertility Study; Fertilization; Funding; Genes; Genetic; Genetic Screening; Germ Cells; Goals; Human; Immunoglobulins; Immunologics; Individual; Infertility; Lead; Ligands; Medical; Membrane Proteins; Methods; Modeling; Molecular; Molecular Analysis; Morphology; Mutation; Nature; Nematoda; Oocytes; Organism; Pathway interactions; Phenotype; Process; Proteins; Reporting; Research; Retinal blind spot; Role; Solid; Sperm Motility; Sperm-Ovum Interactions; Sterility; Work; conditional mutant; design; economic implication; egg; forward genetics; gene discovery; gene function; genetic analysis; genetic approach; genome sequencing; innovation; insight; molecular modeling; mutant; novel; novel strategies; positional cloning; receptor; reproductive; reverse genetics; screening; social implication; sperm cell; sperm function; sperm protein; success; tool; whole genome

RELEVANCE: Fertilization is biological process with important medical, social and economic implications. From extensive study, the events of fertilization are known in some detail. However, the molecular underpinnings of these events generally remain elusive. Most previous work on fertilization has relied on biochemical and immunological approaches. Our work is groundbreaking in the application of classic gentic analsysis to this vital area of research. RATIONALE: Many of the genetic and molecular tools developed for C. elegans are not available or are very difficult to utilize in other organisms traditionally used for studying fertilization. One of the most significant advantages of C. elegans is the ability to isolate and maintain mutants that affect sperm or eggs and no other cells. Previously, through the study of sterile mutants, we have identified some of the first molecules required for productive gamete interactions in C. elegans. We aim to get a better understanding how sperm molecules (SPE-13, SPE-36, and SPE-51) function to insure successful fertilization. The Izumo-like spe-45 and spe-51 genes represent conserved sperm function from worms to humans. Futher, a full understanding of the molecular mechanisms of fertilization is impossible without a more complete inventory of molecular components. Therefore we aim to identify new fertilization molecules through genetic and molecular analysis. Particular emphasis will be placed on the identification of genes required in oocytes. OBJECTIVES: The goal of this proposal is to further our understanding of fertilization in C. elegans by conducting the following experimental aims: 1) Investigate mechanistic connections, functions, and fertilization pathways of the gamete function molecules SPE-13, SPE-36, and SPE-51. 2) Conduct complementary forward and reverse genetic screens for fertilization mutants. We will take advantage of an innovative genetic screening strategy that enriches for gamete function mutants. 3) Determine the molecular nature of new gamete function genes defined by a fertilization defective mutant phenotype. This work will complement fertility studies in other organisms as well as provide insights into the mechanisms of cell-cell interactions and the diversity of reproductive strategies.

相关性：受精是重要的医学，社会的生物学过程 和经济影响。从广泛的研究中，施肥事件在 一些细节。但是，这些事件的分子基础通常仍然存在 难以捉摸。以前关于受精的大多数工作都依赖于生化和免疫学 方法。我们的工作在将经典的绅士分析应用于 这个重要的研究领域。 理由：为秀丽隐杆线虫开发的许多遗传和分子工具 在传统上使用的其他生物中不可用或很难使用 研究施肥。秀丽隐杆线虫最重要的优势之一是能力 分离和维持影响精子或卵和没有其他细胞的突变体。之前， 通过无菌突变体的研究，我们已经确定了一些第一分子 秀丽隐杆线虫中生产配子相互作用所必需的。我们的目标是变得更好 了解精子分子（SPE-13，SPE-36和SPE-51）如何确保 成功的受精。类似Izumo的SPE-45和SPE-51基因代表保守 从蠕虫到人类的精子功能。 futher，对分子的充分理解 没有更完整的分子清单，受精的机制是不可能的 成分。因此，我们旨在通过遗传来鉴定新的受精分子 和分子分析。特别强调基因的识别 在卵母细胞中需要。 目标：该提议的目的是进一步了解我们对 通过进行以下实验目的，在秀丽隐杆线虫中受精：1） 配子功能的机械连接，功能和受精途径 分子SPE-13，SPE-36和SPE-51。 2）进行互补前进和 反向遗传筛选用于受精突变体。我们将利用创新的优势 富含配子功能突变体的基因筛选策略。 3）确定 由受精缺陷定义的新配子功能基因的分子性质 突变表型。这项工作还将补充其他生物的生育研究 因为提供了对细胞 - 细胞相互作用机制的见解和多样性 生殖策略。