Application and extension of dye-based food consumption methodology in Drosophila

基于染料的食物消耗方法在果蝇中的应用和推广

• 批准号: 9980753
• 负责人: MICHAEL S. GROTEWIEL
• 金额: $ 3.88万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9980753
• 关键词: Address; Aging; Behavior; Complex; Consumption; Development; Diet; Disease; Disease Marker; Disease Progression; Drosophila genus; Drosophila melanogaster; Dyes; Eating; Environmental Risk Factor; Equipment; Ethanol; Excretory function; Feeding Methods; Food; Foundations; Genes; Genetic; Goals; Health; Housing; Human; Incidence; Intake; Laboratories; Laboratory Organism; Lead; Longevity; Measures; Metabolism; Methodology; Methods; Minor; Modeling; Modification; Molecular; Musca domestica; Non-Insulin-Dependent Diabetes Mellitus; Nutrient; Obesity; Obesity Epidemic; Obesity associated disease; Organism; Partner in relationship; Physiological Adaptation; Physiology; Prevention; Process; Reagent; Reporting; Resources; Role; Sedation procedure; Solid; Starvation; Time; Tracer; Translating; base; dietary restriction; egg; experimental study; fly; food consumption; improved; response; sex determination

Project Summary/Abstract: Over the past 100+ years, studies in Drosophila melanogaster (Drosophila or flies) have contributed enormously to our understanding of the genetic basis for sex-determination, development, behavior, aging, disease and many other normal as well as pathophysiological processes. More recently, the fly model has also been used to explore the contribution of environmental factors in health and disease. Key among the environmental factors being investigated is diet. Altering the diet of flies has profound effects on their physiology, progression of disease markers and aging. For example, dietary restriction in flies (via reduced concentrations of nutrients) extends lifespan as found in other species. Changes in the fly diet also greatly impact egg-laying (an indicator of resource utilization) and can lead to the development of a type II diabetes-like state. Furthermore, our preliminary studies indicate that diet has a pronounced effect on ethanol sedation behavior in flies. Given the effects of diet in flies, the ongoing global obesity epidemic and the increasing incidence of obesity-related diseases, studies in flies hold tremendous promise for continuing to uncover key mechanisms underlying diet-related phenomena that could ultimately translate into improved prevention and treatment of a multitude of diet-related diseases in humans. We have consequently begun developing dye-based methods (Con-Ex) for measuring food consumption in flies. The overarching goals of the studies in this application are to (i) use our previously established dye-based Con-Ex feeding method with the dye Blue-1 as a food tracer to assess intake of diets that alter longevity—and importantly do so across the lifespans of flies and (ii) extend the utility of the method by identifying and validating dyes in addition to Blue-1 for use in Con-Ex studies. The proposed studies will address an important question related to the effects of diet on longevity and will lay the foundation for more sophisticated dietary consumption analyses in flies.

项目摘要/摘要：在过去的100多年中，果蝇的研究（果蝇或 苍蝇）为我们对性别确定的遗传基础的理解做出了巨大贡献， 发展，行为，衰老，疾病以及许多其他正常以及病理生理过程。更多的 最近，Fly模型还用于探索环境因素在健康方面的贡献和 疾病。正在研究的环境因素中的关键是饮食。改变苍蝇的饮食有深远的 对它们的生理学，疾病标志物的进展和衰老的影响。例如，果蝇的饮食限制 （通过降低的养分浓度）延长了其他物种中发现的寿命。苍蝇饮食的变化 还极大地影响了卵子的卵子（资源利用的指标），并可能导致II型的发展 类似糖尿病状态。此外，我们的初步研究表明饮食对乙醇有明显的影响 苍蝇中的镇静行为。鉴于饮食在苍蝇中的影响，持续的全球肥胖流行和 与肥胖相关疾病的发生率增加，苍蝇的研究具有巨大的希望 发现与饮食相关现象的基本关键机制，最终可以转化为改进 预防和治疗人类中多种与饮食相关的疾病。因此，我们已经开始 开发基于染料的方法（CON-EX）来测量果蝇的食物消耗。总体目标 该应用中的研究是（i）使用我们先前建立的基于染料的con-ex喂养方法 Dye Blue-1是评估改变寿命的饮食摄入量的食物示踪剂，并且重要的是在整个过程中这样做 果蝇的寿命和（ii）除了识别和验证染料外，还扩展了该方法的实用性。 用于参考研究。拟议的研究将解决与饮食影响有关的重要问题 关于寿命，并将为苍蝇进行更复杂的饮食消费分析奠定基础。