Flow Cytometry Shared Resource

流式细胞术共享资源

基本信息

批准号：
9975718
负责人：
DOUGLAS T FEARON
金额：
$ 27.15万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9975718
关键词：
Accounting Aneuploidy Animal Model Apoptosis Cancer Center Cancer Center Support Grant Cancer Center at Cold Spring Harbor Laboratory Cell Cycle Progression Cell Separation Cells Complement Computer Workstations Computer software Consultations Data Data Analyses Equipment Equipment Design Experimental Designs Flow Cytometry Flow Cytometry Shared Resource Fluorescence-Activated Cell Sorting Funding Generations Immune Immunofluorescence Immunologic Individual Laboratories Lasers Malignant Neoplasms Modality Population Population Analysis Research Research Personnel Resource Sharing Role Scanning Services Supporting Cell Training anticancer research base cancer cell cell analyzer computerized data processing experimental analysis experimental study improved innovation instrument instrumentation interest member operation programs protein expression protocol development service utilization single cell analysis single cell sequencing stem cells

项目摘要

Flow Cytometry Shared Resource - Project Summary/Abstract For more than thirty years, the Flow Cytometry Shared Resource has served members of the CSHL Cancer Center, offering access to critical equipment and expertise for cell sorting and cell population analysis. The Shared Resource offers three different state-of-the-art instruments with up to five different lasers for cell sorting and analysis. Expert staff is also available for training in the use of equipment, consultation on experimental design, and assistance with operation of instruments. All of these services are highly technical and require skilled staff. Access to Flow Cytometry has allowed the development of protocols for single cell sequencing, the generation and analysis of innovative animal models, and isolation and characterization of the stem cells and immune cells involved in cancer. The Flow Cytometry Shared Resource is well used by Cancer Center members; 31 members (84% of the membership) used this Shared Resource, accounting for 95% of use. Without the Flow Cytometry Shared Resource it would be extremely difficult for individual investigators to have access to this sophisticated instrumentation and these types of services, which are critical to their research programs. During the last funding period, the laboratory space for the Flow Cytometry Shared Resource has been renovated and expanded, improving the efficiency and reliability of the Shared Resource. In summary, the Shared Resource provides CSHL Cancer Center members with a complete complement of state-of-the-art instrumentation and advanced technical support to help accelerate cancer research and discovery.

流式细胞术共享资源 - 项目摘要/摘要三十多年来，流式细胞术共享资源一直为 CSHL Cancer 成员提供服务中心，提供细胞分选和细胞群分析的关键设备和专业知识。这共享资源提供三种不同的最先进仪器以及多达五种不同的激光器用于细胞分选和分析。专家人员还可提供设备使用培训、实验咨询设计并协助仪器操作。所有这些服务技术性很强，需要熟练的员工。使用流式细胞术可以开发单细胞测序方案，创新动物模型的生成和分析，以及干细胞的分离和表征和参与癌症的免疫细胞。流式细胞术共享资源被癌症中心充分利用会员； 31 名会员（会员的 84%）使用了该共享资源，占使用量的 95%。如果没有流式细胞术共享资源，个别研究人员将很难获得获得这种复杂的仪器和这些类型的服务，这对他们的研究至关重要程序。在上一个资助期间，流式细胞术共享资源的实验室空间已进行了改造和扩建，提高了共享资源的效率和可靠性。总之，共享资源为 CSHL 癌症中心成员提供了一整套最先进的资源仪器和先进的技术支持有助于加速癌症研究和发现。