ShEEP Request for Keyence BZ-X800E All-in-One Fluorescence Microscope

ShEEP 请求购买 Keyence BZ-X800E 一体式荧光显微镜

• 批准号: 9907801
• 负责人: Bruce R. Troen
• 金额: --
• 依托单位: VA WESTERN NEW YORK HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907801
• 关键词: Activities of Daily Living; Acute; Age related macular degeneration; Aging; Animals; Antioxidants; Biological Markers; Carbon Dioxide; Cardiac; Catalytic RNA; Cell Death; Cell physiology; Cells; Chronic; Clinic; Collaborations; Collection; Color; Computer software; Controlled Environment; Cultured Cells; Data; Development; Devices; Dimensions; Disease; Equipment; Fluorescence; Functional disorder; Funding; Future; Generations; Gliosis; Health; Heart Arrest; Hematopoietic; Histologic; Human; Idiopathic Parkinson Disease; Image; Image Analysis; Immersion Investigative Technique; Interval training; Intervention; Kinetics; Manufacturer Name; Measurement; Mediating; Methodology; Microscope; Midbrain structure; Modeling; Monitor; Morphology; Mus; Muscle Cells; Muscle Fibers; Musculoskeletal; Mutation; Myocardial Infarction; Oils; Orphan; Osteoclasts; Outcome; Oxidative Stress; Parkinson Disease; Pathologic; Pathologic Processes; Phase; Physiological; Population; Process; Quality of life; RNA; RNA Interference; Reagent; Research; Research Personnel; Resolution; Retinal Degeneration; Retinitis Pigmentosa; Role; Severities; Sheep; Signal Transduction; Skeletal Muscle; Skeletal bone; Slide; Stem cells; Temperature; Therapeutic; Therapeutic Agents; Therapeutic Intervention; Three-Dimensional Image; Three-Dimensional Imaging; Time; Tissues; Translating; Translational Research; Veterans; Visual; Work; adult stem cell; base; bench to bedside; bone; bone resorbing activity; cardiac repair; dietary supplements; dopaminergic neuron; effective intervention; exosome; flasks; fluorescence imaging; fluorescence microscope; fluorophore; frailty; improved; induced pluripotent stem cell; insight; instrument; live cell imaging; macrophage; movie; novel; parkin gene/protein; prevent; real-time images; repaired; stem; therapeutic candidate; tool; trafficking

Project Abstract The Keyence BZ-X800E All-in-One fluorescent microscope is a unique instrument that provides multi-color fluorescent imaging, 3D image rendering and measurement localization, live cell and time-lapse video generation, automated multi-image collection, and real-time image stitching and image analysis. Thus, the Keyence microscope will be a crucial instrument for VAWNYHS investigators, and the unique features will have immediate benefits for VA-funded projects below. Dr. Troen’s work investigates musculoskeletal aging, frailty, and functional capacity in older veterans. Dr. Troen is conducting both human and animal studies to explore the benefits of high intensity interval training (HIIT) and nutritional supplements on skeletal muscle and bone morphology and function during aging. The Keyence will allow more precise assessments of muscle fibers in a fraction of the time of the current methodology. The Keyence will also be able to generate time-lapse live cell imaging video to observe the formation and activity of bone resorbing osteoclasts, capture the impacts of reagent interventions, and facilitate analysis of bones from experimental animals. Dr. Fliesler’s projects investigate the underlying mechanism of progressive retinal degeneration and visual dysfunction associated with blast overpressure-induced polytrauma, and the impact of novel antioxidants as therapeutic agents to prevent, minimize, or slow the progression of the pathological processes. The Keyence microscope will afford quantitative assessment of histological damage and biomarkers of oxidative stress, cell death, and gliosis. Dr. Sullivan’s study investigates the development of post-transcriptional gene silencing agents such as ribozymes (catalytic RNAs) as candidate therapeutics for orphan retinal degenerations (e.g. retinitis pigmentosa) and common age-related macular degeneration (AMD). The Keyence will provide dramatic new methodologies to observe cellular RNA trafficking and investigate the kinetics and steady-state outcomes of catalytic RNA (ribozyme) therapeutics. Dr. Canty’s study investigates dynamic remodeling during sudden cardiac arrest and cardiac stem cell mediated repair. The Keyence will facilitate characterization of specific hematopoietic subtypes and macrophage polarization and enable characterization of stems in culture to better understand how adult stem cells stimulate endogenous myocyte proliferation. Dr. Feng’s study seeks to understand how mutations of parkin cause the selective degeneration of human nigral dopaminergic neurons and the ensuing Parkinson’s disease. The Keyence will allow the ability to monitor morphological changes and enable imaging of iPSC-derived midbrain DA neurons, to identify impacts of parkin mutations as a model of idiopathic Parkinson’s disease. Dr. Lang’s research seeks to determine the functional role of exosomes in stem cell-mediated cardiac repair. The Keyence microscope will enhance this work by facilitating extremely high resolution immunohistological analysis of murine cardiac tissue following myocardial infarction and evaluating the potential of exosome-based therapy to ameliorate damaged tissues. The range of applications provided by the Keyence BZ-X800E microscope will greatly facilitate our understanding of the underlying acute and chronic processes that result in physiological dysfunction, disease, and health decline. Further, it will be an essential tool for the VAWNYHS researchers to develop effective interventions to minimize the severity, delay the onset, or arrest the progression of frailty and ultimately improve the quality of life in the veteran population.

项目摘要 Keyence BZ-X800E多合一荧光显微镜是一种独特的仪器 提供多色荧光成像，3D图像渲染和测量定位，活细胞 以及延时视频，自动化的多图像收集以及实时图像缝合和 图像分析。那就是钥匙显微镜将是Vawnyhs的关键仪器 调查人员和独特的功能将对下面的VA资助项目产生直接的好处。 Troen博士的工作调查了老年人的肌肉骨骼衰老，脆弱和功能能力 退伍军人。 Troen博士正在进行人类和动物研究，以探索高 强度间隔训练（HIIT）和骨骼肌形态上的营养补充剂 和衰老期间的功能。钥匙将允许对肌肉纤维进行更精确的评估 当前方法的一部分。钥匙也将能够产生延时 活细胞成像视频观察骨骼分离破骨细胞的形成和活性，捕获 试剂干预的影响，并促进对实验动物的骨骼分析。 弗莱斯勒博士的项目调查了渐进永久性变性的潜在机制 和与爆炸过压诱导的多发性杂志有关的视觉功能障碍以及 新颖的抗氧化剂作为治疗剂，以预防，最小化或减慢速度 钥匙显微镜将对组织学进行定量评估 氧化应激，细胞死亡和神经胶质的损伤和生物标志物。 沙利文博士的研究调查了转录后基因沉默剂的发展 例如核酶（催化RNA）作为孤儿永久性变性的候选疗法（例如 色素性视网膜炎）和与年龄相关的黄斑变性（AMD）。钥匙会 提供戏剧性的新方法来观察细胞RNA运输并研究动力学 和催化RNA（核酶）疗法的稳态结局。 Canty博士的研究调查了心脏骤停和心脏的动态重塑 干细胞介导的修复。钥匙将喜欢特定造血的特征 亚型和巨噬细胞极化，并使植物在培养物中的表征更好 了解成年干细胞如何刺激内源性心肌细胞增殖。 Feng博士的研究试图了解Parkin的突变如何引起选择性变性 人类多巴胺能神经元和随之而来的帕金森氏病的作用。钥匙将允许 监测形态变化并启用IPSC衍生的中脑DA成像的能力 神经元，以确定帕金突变的影响是特发性帕金森氏病的模型。 Lang博士的研究旨在确定外泌体在干细胞介导的功能作用 心脏修复。钥匙显微镜将通过促进极高的高度来增强这项工作 心肌梗塞后鼠心脏组织的分辨率免疫组织学分析和 评估基于外泌体的治疗来改善受损组织的潜力。 Keyence BZ-X800E显微镜提供的应用程序范围将极大地促进 我们对导致生理的潜在急性和慢性过程的理解 功能障碍，疾病和健康下降。此外，它将是Vawnyhs的重要工具 研究人员制定有效的干预措施，以最大程度地减少严重性，延迟发作或阻止 脆弱的发展，并最终改善了退伍军人人口的生活质量。