ShEEP Request for Laser Scanning Microscope (LSM) 880

ShEEP 请求激光扫描显微镜 (LSM) 880

• 批准号: 9907067
• 负责人: Wendy B Bollag
• 金额: --
• 依托单位: CHARLIE NORWOOD VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907067
• 关键词: 10 year old; Area; Arrestins; Atherosclerosis; Basic Science; Biological Assay; Biomedical Research; Cell physiology; Cells; Cellular biology; Chronic; Collaborations; Collection; Computer software; Copper; Corneal Injury; Data; Detection; Dimensions; Elements; Ensure; Equipment; Fluorescence Resonance Energy Transfer; Funding; Future; Health; Image; Imagery; Imaging Techniques; Inflammation; Kidney; Laboratories; Lasers; Light; Lighting; Location; Manufacturer Name; Microscope; Microscopy; Microtomy; Mission; Modeling; Nicotinic Acids; Noise; Optics; Organelles; Outcome Measure; Parkinson Disease; Phosphatidylglycerols; Photons; Positioning Attribute; Principal Investigator; Productivity; Proteins; Psoriasis; Research; Research Personnel; Research Project Grants; Resolution; Retinal Diseases; Role; Sampling; Scanning; Science; Scientist; Services; Shapes; Sheep; Signal Transduction; Site; Specimen; Speed; System; Technology; Temperature; Thick; Time; Tissues; Translational Research; Universities; Update; Veterans; Width; Wound Healing; adaptive optics; aquaporin 3; arginase; career; confocal imaging; detector; experimental study; fluorophore; improved; injury and repair; instrument; interest; kidney repair; light emission; muscle invasive bladder cancer; new technology; optical imaging; two-dimensional; two-photon; waiver

The requested instrument is a laser-scanning microscope (LSM model 880), a confocal microscope manufactured by Zeiss, which is needed to replace an approximately 10- year-old Zeiss LSM 510, that is no longer supported by the company because it is outdated. The updated LSM 880 microscope allows high-resolution confocal imaging of multiple fluorophores, permitting visualization of the subcellular location of proteins of interest and/or the co-localization of these proteins with other proteins or cellular organelles. Importantly, the LSM 880 is equipped with AiryScan (Airy Disk) technology; this newer technology employs many single and independent detector elements, with each of these 32 detectors acting like a very small pinhole. The confocal pinhole itself remains open and does not block light; thus, all of the photons from the entire Airy disk are collected. The signals from all of the detector elements are then reassigned to their correct position, producing an image with an increased signal-to-noise ratio (SNR) and enhanced resolution. Thus, the AiryScan allows the collection of all of the emitted light and combines super-resolution with high speed and sensitivity. The LSM 880 can also be upgraded to permit two-photon technology, which would allow confocal imaging of thicker specimens, as well as cell wound healing assays. This instrument will support at least 7 VA Merit-funded research projects from 6 VA Principal Investigators: (1) #CX001357: “Epidermal aquaporin-3 in psoriasis” and #BX004735: “Phosphatidylglycerol to promote corneal wound healing and inhibit chronic inflammation” (PI: Dr. Wendy Bollag); (2) #BX001232: “Role of copper transporters in atherosclerosis” (PI: Dr. Tohru Fukai); (3) #BX001233: “Mechanisms of traumatic retinopathy: Role of arginase” (PI: Dr. Ruth Caldwell); (4) #BX003862: “Role of B- arrestins in muscle-invasive bladder cancer” (PI: Dr. Balakrishna Lokeshwar); (5) #BX000319 “Ischemic kidney injury and kidney repair” (PI: Dr. Zheng Dong; Dr. Dong is also a Senior VA Research Career Scientist); and (6) #RX001613: “GPR109A and Parkinson's disease: Role of niacin in outcome measures” (PI: Dr. Chandramohan Wakade). The Zeiss LSM 880 is a state-of-the-art system with a proven track record, allowing high-resolution optical sections, and it is expected that this shared instrument will promote collaborations between VA Merit-funded investigators and increase research productivity thus advancing the VA mission to improve veterans’ health.

所需仪器是激光扫描显微镜（LSM 型号 880）、共焦显微镜 蔡司制造的显微镜，需要更换大约 10- 一年前的 Zeiss LSM 510，该公司不再支持该产品，因为它 更新的 LSM 880 显微镜可实现高分辨率共焦成像。 多个荧光团，允许蛋白质的亚细胞位置可视化 这些蛋白质与其他蛋白质或细胞的兴趣和/或共定位 重要的是，LSM 880 配备了 AiryScan（艾里斑）技术； 这项新技术采用了许多单一且独立的探测器元件， 这 32 个探测器中的每一个都像一个非常小的针孔，共焦针孔本身。 保持开放并且不阻挡光线；因此，来自整个艾里斑的所有光子 然后，来自所有检测器元件的信号被重新分配给它们。 正确的位置，生成具有更高信噪比 (SNR) 的图像，并且 因此，AiryScan 可以收集所有发射的光。 LSM 880 还可以将超分辨率与高速和灵敏度相结合。 升级以允许双光子技术，这将允许共焦成像 该仪器将支持较厚的样本以及细胞伤口愈合测定。 至少 7 个由 6 名 VA 首席研究员资助的 VA Merit 资助的研究项目：(1) #CX001357：“牛皮癣中的表皮水通道蛋白-3”和#BX004735： “磷脂酰甘油促进角膜伤口愈合并抑制慢性 （PI：Wendy Bollag 博士）；(2) #BX001232：“铜转运蛋白在 （PI：Tohru Fukai 博士）；(3) #BX001233：“创伤性机制 视网膜病变：精氨酸酶的作用”（PI：Ruth Caldwell 博士）；(4) #BX003862：“B- 的作用 肌肉浸润性膀胱癌中的抑制素”（PI：Balakrishna Lokeshwar 博士）（5）； #BX000319“缺血性肾损伤与肾脏修复”（PI：郑东博士；董博士是 也是一名高级 VA 研究职业科学家）；以及 (6) #RX001613：“GPR109A 和 帕金森病：烟酸在结果测量中的作用”（PI：Chandramohan 博士 Zeiss LSM 880 是一款最先进的系统，拥有良好的记录， 允许高分辨率光学切片，并且预计这种共享仪器 将促进 VA Merit 资助的调查人员之间的合作，并增加 研究生产力，从而推进退伍军人事务部改善退伍军人健康的使命。