ShEEP Request for IVIS SPECTRUMCT, 2D and 3D Optical In Vivo Tomography System.

ShEEP 请求 IVIS SPECTRUMCT、2D 和 3D 光学体内断层扫描系统。

• 批准号: 9907225
• 负责人: Hee-Jeong Im Sampen
• 金额: --
• 依托单位: JESSE BROWN VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907225
• 关键词: 3-Dimensional; Advisory Committees; Alcohol abuse; Alzheimer' s Disease; Anatomy; Animal Model; Animals; Anxiety; Basic Science; Bioluminescence; Chicago; Chronic; Clinical Investigator; Clinical Trials Design; Data; Data Set; Degenerative polyarthritis; Detection; Diabetic Neuropathies; Digital Imaging and Communications in Medicine; Disease; Dose; Drug Kinetics; Drug toxicity; Ensure; Equipment; Evaluation; Fluorescence; Fluorochrome; Foundations; Funding; Generations; Goals; Grant; Image; Image Analysis; Image Enhancement; Industry; Inflammatory; Interdisciplinary Study; Intuition; Laboratories; Longitudinal Studies; Magnetic Resonance Imaging; Manufacturer Name; Medical center; Mental Depression; Metabolic Diseases; Minor; Modality; Modeling; Molecular; Motion; Multiple Sclerosis; Obesity; Optical Tomography; Optics; Orthopedics; Pain; Pain Research; Parkinson Disease; Performance; Pharmacodynamics; Pilot Projects; Positron-Emission Tomography; Procedures; Research; Research Personnel; Research Support; Scanning; Services; Sheep; Surface; System; Systems Analysis; Technical Expertise; Techniques; Technology; Three-Dimensional Image; Time; Tissue Harvesting; Tracer; Training; United States National Institutes of Health; Universities; Veterans; Work; X-Ray Computed Tomography; animal facility; biomarker identification; cherenkov imaging; complex biological systems; design; detector; experience; gastrointestinal; germ free condition; gut microbes; imaging capabilities; imaging modality; imaging software; imaging system; in vivo; in vivo imaging; in vivo imaging system; in vivo optical imaging; insight; instrument; luminescence; multidisciplinary; novel therapeutics; optical imaging; pre-clinical; pre-clinical research; programs; radiotracer; real-time images; reconstruction; research and development; screening; single photon emission computed tomography; therapy development; tomography

Project Summary/Abstract We propose to acquire an IVIS (IN VIVO IMAGING SYSTEM) SPECTRUMCT. It has multiple functions, including 2D Bioluminescence, 3D Bioluminescence/ Fluorescence Tomography, PET, CT and MRI functions for small and large animals, detection of in vivo Ca2+ influx that can be useful for pain study, and drug toxicity evaluation that is critical for identifying and developing new drugs. Presently there is no any highly sensitive tomographic optical imaging systems of this kind available to researchers in Jesse Brown VAMC to support VA-funded studies. This instrument will be the first IVIS SPECTRUMCT at the Jesse Brown VAMC, and will significantly accelerate data generation for the VA-funded studies by providing sophisticated in vivo analytical capabilities that are currently unavailable at the Jesse Brown VAMC or nearby universities. By using the new analytical in vivo imaging capabilities, we also expect to use significantly fewer animals for our proposed studies by eliminating time-course studies that require sacrificing animals followed by tissue harvest. Approximately 85% of the instrument time will be utilized by these VA-funded major users, and the remaining time will be used to support NIH-, DoD-funded or other pilot projects. This instrument will be located in the VA Animal Facility where the VA investigators can conduct in vivo imaging analyses without taking the animals out of the animal facility, which is designed to house specific pathogen free animals. Access to the instrument by the major/minor users will be ensured through the administrative oversight of an Advisory Committee appointed by the Chair of the R&D Committee. Administrative/technical service will be provided by Dr. Sampen who has imaging analyses experience for more than a decade, and by a coordinator, Dr. Natarajan Anbazhagan who has over 4 years of experience in directly operating the IVIS SPECTRUMCT system. To ensure that the equipment is operated safely and appropriately maintained with maximum efficiency, Drs. Sampen and Natarajan Anbazhagan will be further trained by the Perkin Elmer Technical team with intensive training courses.

项目摘要/摘要 我们建议获得IVI（体内成像系统）光谱。它有多个 功能，包括2D生物发光，3D生物发光/荧光断层扫描，PET， CT和MRI的功能适用于小动物和大型动物，检测可以是体内Ca2+流入 对于疼痛研究和药物毒性评估有用，这对于识别和开发新的至关重要 毒品。目前，此类没有任何高度敏感的层析成像系统 杰西·布朗VAMC的研究人员可用于支持VA资助的研究。该乐器将 成为Jesse Brown VAMC上的第一个IVIS Spectrumct，并将显着加速数据 通过提供了体内分析能力，生成VA资助的研究 目前在Jesse Brown VAMC或附近的大学中不可用。通过使用新 分析体内成像功能，我们还期望为我们的动物使用明显较少的动物 提出的研究通过消除需要牺牲动物的时间课程研究，然后 组织收获。这些VA资助将使用大约85％的仪器时间 主要用户以及剩余时间将用于支持NIH-，DOD资助或其他飞行员 项目。该乐器将位于VA动物设施中 进行体内成像分析，而无需将动物从动物设施中取出 设计用于容纳特定的无病原体动物。专业/辅修器访问该乐器 将通过对任命的咨询委员会的行政监督来确保用户 由研发委员会主席。行政/技术服务将由博士提供。 拥有成像分析经验已有十多年的SAMPEN，由协调员Dr. Natarajan Anbazhagan在直接运营IVI的经验中拥有超过4年的经验 Spectrumct系统。确保设备安全，适当地操作 以最大的效率维持。 Sampen和Natarajan Anbazhagan将进一步 由Perkin Elmer技术团队培训，由强化培训课程进行。