ShEEP Request for Fully Automated Perifusion System

ShEEP 请求全自动灌注系统

• 批准号: 9905782
• 负责人: Steven Emanuel Kahn
• 金额: --
• 依托单位: VA PUGET SOUND HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9905782
• 关键词: Adipose tissue; Animal Model; Area; Blood Vessels; Brain; Businesses; Caring; Cell Survival; Cell physiology; Cells; Collection; Communities; Custom; Data Analyses; Diabetes Mellitus; Disease; Ensure; Environment; Equipment; Evaluation; Funding; Funding Agency; Generations; Health; Healthcare Systems; Hour; In Vitro; Inflammatory; Infrastructure; Insulin; Intuition; Islets of Langerhans; Leukocytes; Liver; Liver diseases; Maintenance; Malignant Neoplasms; Manufacturer Name; Measurement; Microfluidics; Minor; Muscle; Neurodegenerative Disorders; Neuropeptides; Normal tissue morphology; Obesity; Output; Paper; Physiological; Production; Protocols documentation; Reporting; Reproducibility; Research; Research Infrastructure; Research Personnel; Resources; Robotics; Sampling; Savings; Schedule; Scientist; Services; Sheep; Slice; Specimen; Stimulus; System; Technology; Temperature; Time; Tissue Sample; Tissues; Training; Traumatic Brain Injury; Travel; United States National Institutes of Health; Veterans; base; cell type; design; diabetic; equipment acquisition; experience; experimental study; human model; improved; instrument; meetings; preservation; prevent; programs; response; sample collection; sound; stressor; time use; touchscreen

ABSTRACT This shared equipment proposal seeks to acquire a state-of-the-art fully automated perifusion system at VA Puget Sound Health Care System (VAPSHCS. The proposed instrument (a Biorep Technologies PERI4- FA-115 perifusion system) comprises an integrated perifusion system which simultaneously delivers solutions to multiple cell/tissue samples under highly controlled temperature, time and flow-rate conditions. The system also includes an integrated, robotic sample collection module, which allows collection of up to 36 samples simultaneously, and up to 1,920 samples per experiment. Finally, the system is fully automated, providing significant increases in efficiency and time-savings over conventional approaches for studies of this kind. This instrument will be housed, managed and maintained within the Cell Function and Analysis Sub-Core which is located at the Seattle Division of VAPSHCS. The proposed equipment purchase expands VAPSHCS’s research infrastructure, meeting an unmet need for the research program (described below). Specifically, there is no instrument on station at present that is capable of this functionality. Further, there is only one similar instrument within the Seattle area. This instrument is heavily used and located several miles away, precluding its use for time-sensitive studies for which this instrument is designed. Thus, investigators at VAPSHCS currently have no capability to perform the studies supported by the proposed perifusion system. The Biorep perifusion system enables real time, in vitro analysis of cell or tissue samples in response to a range of stimuli. This system is amenable to almost any cell or tissue type and is ideally suited for studies looking at dynamic changes in the production of secreted analytes or changes in cell viability. Importantly, it is widely applicable to our diverse community of researchers which includes those working in diabetes, cancer, obesity, liver disease, traumatic brain injury and neurodegenerative diseases. Twelve investigators on station working across these areas have an immediate need for the proposed instrument. Nine of these VA-funded (using ~75% of instrument time). These include 6 Major users (whose usage will total 28 h/week). The 3 remaining VA-funded investigators together with 3 additional users (with funding by agencies such as NIH), comprise the Minor user group (6 total; usage of 16 h/week. We present a comprehensive plan for the sharing, administration, scheduling (including use outside of regular business hours) and care of the instruments. A summary of some of the different uses of this system is given here (and detailed in the Research Plan): · Assessment of dynamic insulin release from pancreatic islets in response to a variety of physiological stimuli, using samples from normal or diabetic humans or animal models · Determination of inflammatory molecule production from vascular cells, leukocytes, liver, muscle and adipose tissue specimens under normal and disease conditions · Evaluation of neuropeptide release from brain slices · Measurement of cell viability in response to stressors in all of the above cell types In summary, this new instrument package will provide a state-of-the-art scientific platform to support many investigators based at VAPSHCS in Seattle. The instrument will be located and managed by the established Cell Function and Analysis Sub-Core, which already includes well-developed administrative and technical infrastructure for the management, scheduling and support of the Core’s services.

抽象的 该Sharede设备提案旨在在系统的系统中获得最新的全自动时期 VA Puget声音医疗保健系统（VAPSHCS。支撑仪器（Biorep Technologies peri4-- FA-115颗粒化系统）包括一个集成的颗粒化系统，该系统同时提供解决方案 在高度控制的温度，时间和流率条件下进行多个细胞/组织样品。 还包括一个集成的机器人样品收集模块，该模块最多可收集36个样品 同时每个实验最多可容纳1,920个样品。 对于此类研究的常规方法，效率和节省时间的显着提高。 仪器将在细胞功能中容纳，管理和维护，分析子核为 位于VAPSHC的TLE部门。 支撑设备购买扩大了VAPSHCS的研究基础设施，满足了未满足的需求 对于研究计划（以下描述）。 能够进一步的功能。 仪器被大量使用，并将其定位在英里范围之外，排除了时间敏感的研究 该工具是设计的。 由支撑性鉴定系统支持的研究。 Biorep Perification系统可以实时对细胞或组织样品进行实时分析，以响应A 刺激范围几乎适合于任何细胞类型，非常适合研究 查看分泌分析物的产生或细胞生存力的变化的动态变化 广泛适用于我们多样化的研究人员社区，其中包括从事糖尿病，癌症，癌症的研究人员 肥胖，肝病，创伤性脑损伤和神经退行性疾病。 在整个区域工作都需要立即需要该拟议的工具。 （使用约75％的仪器时间） 剩余的VA资助的调查人员以及其他3个用户（NIH等机构的资助） 组成次要用户组（总计6;使用16小时/周。我们提出了共享的全面计划， 管理，日程安排（在正常工作时间以外使用）和对工具的护理。 对系统的某些不同用途进行了摘要（在研究计划中详细介绍）： ·根据多种 使用正常或糖尿病人类或动物模型的样品生理局部刺激 ·从血管细胞，白细胞，肝脏，肌肉中测定炎症分子的产生 正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常正常态 ·对脑切片中神经肽释放的评估 ·响应上述所有细胞类型的应激源的细胞活力测量 总而言之，这个新的仪器包将提供一个最先进的科学平台，以支持许多 Searchle中的VAPSHC的调查人员将由已建立的仪器找到和管理 细胞功能和分析子核，该核核心已经包括发达的行政和技术 管理，安排和支持核心服务的基础架构。