Role of cell cycle regulators in differentiation

细胞周期调节因子在分化中的作用

• 批准号: 8619666
• 负责人: Patrizia Casaccia
• 金额: $ 36.71万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-15 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8619666
• 关键词: Affect; Binding; Cancer Biology; Cell Cycle; Cell Cycle Proteins; Cell Differentiation process; Cells; ChIP-seq; Chromatin; Chromatin Structure; Co-Immunoprecipitations; Collaborations; Complex; Data; Deacetylation; Development; Discipline; Dominant-Negative Mutation; Down-Regulation; E2F1 gene; Energy Metabolism; Enzymes; Family; Family member; Funding; Gene Cluster; Gene Expression; Gene Expression Profile; Gene Targeting; Genes; Genetic; Glioblastoma; Grant; HDAC1 gene; Histone Acetylation; Histones; In Vitro; Internal Ribosome Entry Site; Kinetics; Knowledge; Link; Malignant Neoplasms; Mediating; Metabolic; Modeling; Molecular; Mus; Nodal; Oligodendroglia; Oncogenes; Pathology; Physical condensation; Physiological; Proliferating; Proteins; Regulator Genes; Role; Shapes; Sorting - Cell Movement; Specificity; Stem cells; Testing; Time; Transcriptional Regulation; Up-Regulation; Validation; c-Myc Staining Method; cancer cell; cdc Genes; chromatin immunoprecipitation; developmental neurobiology; gene repression; histone acetyltransferase; in vivo; interest; member; metaplastic cell transformation; mouse model; myelination; nervous system disorder; neuro-oncology; neurodevelopment; novel; novel therapeutics; nuclear reprogramming; oligodendrocyte lineage; prevent; progenitor; programs; repaired

DESCRIPTION (provided by applicant): Our lab has identified an interesting relation between members of the Myc and E2f family of cell cycle regulators and chromatin changes and transcriptional regulation of differentiation of oligodendrocyte progenitors. We now propose to use primary cultures and genetic mouse models to test a new mechanistic model of oligodendrocyte differentiation. The results from the proposed experimental plan are likely to advance our knowledge not only in neural development and repair, but also in cancer and nuclear reprogramming and have wide implications for the development of novel therapeutic strategies.

描述（由申请人提供）：我们的实验室已经确定了细胞周期调节因子 Myc 和 E2f 家族成员与染色质变化以及少突胶质细胞祖细胞分化的转录调节之间的有趣关系。我们现在建议使用原代培养物和遗传小鼠模型来测试少突胶质细胞分化的新机制模型。拟议的实验计划的结果可能不仅会增进我们在神经发育和修复方面的知识，而且还会增进在癌症和核重编程方面的知识，并对新型治疗策略的开发具有广泛的影响。