Uncovering the functional potential of the developing peri-implant microbiome

揭示正在发育的种植体周围微生物组的功能潜力

基本信息

批准号：
9765286
负责人：
Shareef Majed Dabdoub
金额：
$ 15.6万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-16 至 2022-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9765286
关键词：
Address Affect Age American Anatomy Area Awareness Biological Markers Chronic Clinical Clinical Research Communities Complex Computer Analysis Data Dental Dental Implants Dental endosseous implant insertion Dentures Development Diagnosis Disease Ecosystem Environment Esthetics Etiology Event Exhibits Fixed Partial Denture Future Genes Gingivitis Goals Health Histologic Impairment Implant Individual Inflammatory Investigation Knowledge Libraries Maintenance Measures Metagenomics Microbial Biofilms Microbiology Modality Modeling Monitor Mucositis National Institute of Dental and Craniofacial Research Oral Outcome Pathway interactions Patients Periodontitis Prevention Prevention strategy Process Property Public Health Recurrence Research Source Structure Surveys Testing Therapeutic Time Tissues Tooth Loss Tooth structure Translations bacteriome biomaterial compatibility clinical care cost effective data mining deep sequencing effective therapy expectation implant material improved member metagenome microbial microbial community microbiome novel peri-implantitis periodontopathogen personalized care personalized medicine prevent restoration restorative material specific biomarkers success targeted biomarker therapeutic target therapy design therapy outcome tool

项目摘要

Abstract Tooth loss is a surprisingly common condition with nearly 60% of Americans over the age of 50 and more than 25% over the age of 19 missing more than 10 teeth, according to the NIDCR. This represents millions of people with impaired masticatory function, phonetic ability, and aesthetics. In the last few decades, osseointegrated implant supported restorations have developed into an often-superior alternative to the traditional removable dentures or fixed bridges. With nearly 2 million dental implants placed each year, and projected increases of 15% annually, dental implants have emerged as a well-accepted treatment modality to restore form and function. While endosseous implants have proven highly reliable and provide increased function over alternatives, their success is dependent on maintenance of the surrounding healthy tissue. Unfortunately, two microbially driven inflammatory diseases often accompany these restorations: Peri-implant mucositis (affecting 50% of implants in 80% of patients) and peri-implantitis (10% of implants in 20% of patients). Clinically, while these conditions mimic gingivitis and periodontitis respectively and have been treated as such, outcomes of therapies have been modest with disturbingly high rates of recurrence. Furthermore, not only has clinical research focused primarily on studies of disease, investigations have examined microbial community composition alone and, until recently, been approached using tools of limited scope. Here we propose the first longitudinal high-throughput, open-ended metagenomics study of the healthy peri- implant microbiome with the following aims: Identifying bacterial functions critical to the formation of the peri- implant microbiome, investigating the validity of the microbial reservoir hypothesis at a functional metagenomic level, and measuring congruence between peri-implant and periodontal colonization dynamics. These studies represent critical discovery-centered examinations targeted at establishing the essential properties of health and the integral functional pathways involved in an important clinical environment affecting millions of patients worldwide. With their completion, we expect to have established a library of critical development markers at key points in the development of the nascent peri-implant metagenome and how they differ from the community and functional makeup of the healthy periodontal metagenome. These will serve as the basis for future investigations into potential therapeutic targets and biomarkers, enabling the development of sensitive, personalized care options that will ultimately improve on current clinical outcomes.

抽象的牙齿脱落是一种令人惊讶的常见状况，近60％的美国人超过50岁，超过根据NIDCR的数据，在19岁以上的19岁以上，有25％的牙齿。这代表数百万具有咀嚼功能，语音能力和美学功能受损的人。在过去的几十年中，骨整合植入物支持的修复体已发展为经常偏远的替代品传统的可移动假牙或固定桥梁。每年都有近200万个牙科植入物，并且预计每年增加15％的增加，牙科植入物已成为一种良好接受的治疗方式还原形式和功能。虽然室内植入物已被证明是高度可靠的，并提供了与替代方案相比的功能，但它们的功能成功取决于维持周围健康组织。不幸的是，两个微生物驱动炎症性疾病通常伴随着这些修复体：种植体粘膜炎（影响50％的植入物在80％的患者中）和种植体周围炎（20％的患者植入物的10％）。临床上，尽管这些条件分别模仿牙龈炎和牙周炎，并被治疗，疗法的结果具有复发率令人不安。此外，不仅临床研究集中主要关于疾病的研究，研究仅检查了微生物群落组成，并且，直到最近，还使用有限范围的工具进行了处理。在这里，我们提出了第一个纵向高通量，开放式的宏基因组学研究植入物微生物组的以下目的：鉴定细菌功能对形成围场至关重要植入物微生物组，研究了功能性元基因组学上微生物储层假说的有效性水平，并测量种植体周围和牙周定殖动力学之间的一致性。这些研究代表了以发现为中心的关键以发现为中心的检查健康的特性和影响重要的临床环境中涉及的整体功能途径全球数百万名患者。随着他们的完成，我们希望建立一个关键的库新生植入植物元基因组开发的关键点的开发标记及其如何与社区和健康牙周元基因组的功能构成不同。这些将作为未来对潜在治疗靶标和生物标志物进行调查的基础，从而实现了发展敏感的个性化护理选择最终将改善当前的临床结果。