Behavioral Core

行为核心

• 批准号: 9703531
• 负责人: WILLIAM MAIXNER
• 金额: $ 27.35万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-20 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9703531
• 关键词: Acetone; Acute Pain; Aftercare; Animal Model; Anxiety; Behavior; Behavioral; Behavioral Assay; Catechol O-Methyltransferase; Clinical Trials; Complementary therapies; Data; Data Reporting; Development; Docosahexaenoic Acids; Electroacupuncture; Enzymes; Fish Oils; Floor; Fracture; Future; Hair; Human Resources; Hyperalgesia; Inflammation; Inflammatory; Lead; Leg; Measurement; Measures; Mechanics; Mental Depression; Modeling; Molecular; Natural Products; Orofacial Pain; Pain; Persistent pain; Postoperative Pain; Process; Reflex action; Reproducibility; Research; Resolution; Rodent; Sensory; Stress; Supervision; Swimming; System; Testing; Time; Tissue Sample; Training; Translations; Trigeminal Pain; Trigeminal System; Trigeminal nerve structure; allodynia; behavior measurement; behavior test; chemotherapy; chronic pain; clinical pain; clinically relevant; comorbidity; conditioned place preference; design; electronic data; experience; mechanical allodynia; mouse model; neuroinflammation; orofacial; pain behavior; pain model; painful neuropathy; programs; sex; spontaneous pain; success; vocalization

The measurement of behavioral signs of evoked and spontaneous pain in rodents is essential to the success of all the 4 subprojects in this program. The Behavioral Core includes 8 newly designed test rooms in a new research Building (MSRBIII). All the behavioral tests in Aim 1 and Aim 2 of all 4 projects will be conducted blindly by three well-trained technicians. All the behavioral data will be saved electronically, analyzed by two statisticians, and shared with Project PIs. We will measure evoked pain such as mechanical allodynia and cold allodynia in both sexes in 4 clinically relevant mouse models of pain. We will also test ongoing pain using conditioned place preference and avoidance (CPP and CPA). We will also measure pain-associated co- morbidity, such as anxiety and depression. We will test the hypothesis that swim stress can prolong the duration of pain. Pain-related behaviors will be tested before and after complementary treatments such as electroacpuncture and DHA. The central hypothesis of this Program Project is that multiple complementary approaches, including electroacupuncture and natural product DHA, may modify distinct inflammation and neuroinflammation processes. We will test our hypotheses in four clinically relevant animal models via the following specific aims: Specific Aim 1: Define the time course of neuropathic pain, postoperative pain, functional pain, and trigeminal pain in 4 clinically relevant mouse models and test the effects of stress and sex on the duration of pain in these models; Specific Aim 2: Ascertain the effects of pre-treatment and post- treatment of EA, aEA, DHA, and RvD1 on neuropathic pain, postoperative pain, functional pain, and trigeminal pain in 4 mouse models; Specific Aim 3 (Exploratory): Develop operant behavioral assays for non-reflexed ongoing pain. We will develop and optimize operant measurement of orofacial pain using Orofacial Stimulation of Ugo Basile. We will also optimize vocalization for detecting acute and chronic pain. Testing behavior in different animal models by the same experienced personnel in the Behavioral Core and collecting the data using electronic-data-report-system will enhance the Scientific Rigor and Reproducibility of the PPG.

测量啮齿动物诱发和自发疼痛的行为迹象对于成功至关重要 该计划的所有 4 个子项目。行为核心包括 8 个新设计的测试室，位于一个新的 研究大楼（MSRBIII）。将进行所有 4 个项目的目标 1 和目标 2 中的所有行为测试 由三名训练有素的技术人员盲目地进行。所有行为数据都将以电子方式保存，并由两个人进行分析 统计学家，并与项目 PI 共享。我们将测量诱发的疼痛，例如机械性异常性疼痛和寒冷 4 个临床相关小鼠疼痛模型中两性的异常性疼痛。我们还将使用以下方法测试持续的疼痛 条件性地点偏好和回避（CPP 和 CPA）。我们还将测量与疼痛相关的协同作用 发病率，例如焦虑和抑郁。我们将检验游泳压力可以延长寿命的假设 疼痛持续时间。将在补充治疗之前和之后测试与疼痛相关的行为，例如 电针和DHA。该计划项目的中心假设是多重互补 包括电针和天然产物 DHA 在内的方法可能会改变不同的炎症和 神经炎症过程。我们将通过以下方法在四种临床相关动物模型中测试我们的假设 具体目标如下： 具体目标 1：定义神经性疼痛、术后疼痛、 在 4 个临床相关小鼠模型中进行功能性疼痛和三叉神经痛，并测试压力和性别的影响 这些模型中疼痛的持续时间；具体目标 2：确定治疗前和治疗后的效果 EA、aEA、DHA 和 RvD1 治疗神经性疼痛、术后疼痛、功能性疼痛和三叉神经痛 4种小鼠模型的疼痛；具体目标 3（探索性）：开发非反射行为的操作性分析 持续的疼痛。我们将使用口面部刺激来开发和优化口面部疼痛的操作性测量 乌戈·巴塞尔。我们还将优化发声以检测急性和慢性疼痛。测试行为 不同的动物模型由相同经验丰富的人员在行为核心中收集数据 使用电子数据报告系统将提高 PPG 的科学严谨性和可重复性。