Critical resources provided by UNMC RAP biorepository stimulate cancer research

UNMC RAP 生物样本库提供的关键资源促进癌症研究

• 批准号: 9757723
• 负责人: Paul M Grandgenett
• 金额: $ 10.68万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-16 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9757723
• 关键词: Address; Archives; Ascites; Autopsy; Award; Blood; Cancer Center; Cancer Research Project; Cell Culture Techniques; Cessation of life; Collaborations; Collection; DNA; Disease; Distant Metastasis; Drug or chemical Tissue Distribution; Early Detection Research Network; Freezing; Funding; Grant; Harvest; Hour; Human; Individual; Institution; International; Knowledge; Laboratories; Leadership; Lesion; Liquid substance; Malignant neoplasm of pancreas; Medical center; Molecular; Nebraska; Neoplasm Circulating Cells; Organ; Organoids; Pancreas; Patients; Play; Premalignant; Primary Neoplasm; Procedures; Process; Proteins; RNA; Recording of previous events; Research; Research Assistant; Research Personnel; Research Project Grants; Research Training; Residual state; Resources; Role; Sampling; Ships; Specialist; Specimen; TNFRSF5 gene; Time; Tissues; Universities; Work; Xenograft procedure; anticancer research; base; biobank; blood-based biomarker; experience; innovation; professor; programs; repository; sample collection; success; tumor microenvironment

Project Summary The highly innovative Rapid Autopsy Pancreas (RAP) program addresses a major limitation in pancreatic cancer research: the lack of quality human specimens for pancreatic cancer representing all stages of disease. Our program of rapid collection of large quantities of all organs and fluids that initiates within an hour of death and is completed within five hours is a unique program in the world. The repository generated from these autopsies allows for the capture of the complete disease history present at the time death and ultimately allows for collection of samples representing the full spectrum of disease from premalignant lesions, primary tumor and metastatic disease. The RAP program is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreatic cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center of UNMC is Dr. Michael A. Hollingsworth. Dr. Paul Grandgenett, a Research Assistant Professor under the aegis of Dr. Hollingsworth's, is the director of the RAP program and the applicant for this Research Specialist Award. The RAP program operates within the Pancreatic Cancer Research Program at UNMC, and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending U01 grants from the Early Detection Research Network; a U54 grant as part of the Tumor Microenvironment Network; and several R01 grants to individual investigators. A key role of Dr. Grandgenett is to work with collaborators to determine the samples that are needed for a research project, to modify our SOP as needed to obtain those samples, and then to provide those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of the patient samples available, as well as the ability to interact on a scientific level with each investigator. Specimens collected include primary tumor, residual unaffected pancreas, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites. These tissues and fluids are rapidly harvested under an SOP that aims to maintain cellular and molecular integrity of the tissue. Selected fresh samples are processed immediately or used for live cell culture/xenograft procedures. These procedures are determined in advance and are based on the needs of funded projects both at UNMC and at other institutions. Currently, we ship fresh samples to other laboratories conducting research on blood based biomarkers (proteins, RNA, DNA), circulating tumor cells, cell cultures including organoids, and other processes. The remaining samples are frozen, maintained in long-term storage facilities and made available through request and collaboration for local, national and international pancreatic cancer studies. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens.

项目概要 高度创新的快速尸检胰腺 (RAP) 计划解决了胰腺的主要局限性 癌症研究：缺乏代表各个疾病阶段的胰腺癌优质人体标本。 我们在死亡一小时内启动快速收集大量所有器官和液体的计划 并且在五个小时内完成是世界上独一无二的项目。从这些生成的存储库 尸检可以捕获死亡时存在的完整疾病史，并最终允许 用于收集代表癌前病变、原发肿瘤的全谱疾病的样本 和转移性疾病。 RAP 项目是内布拉斯加大学医学中心的核心组成部分 (UNMC) 胰腺癌研究项目，其任务是收集、存档和分发 来自最近去世的胰腺癌患者的组织和液体。单位主任 UNMC 弗雷德和帕梅拉·巴菲特癌症中心的胰腺癌研究项目由 Michael 博士负责 A.霍林斯沃斯。 Paul Grandgenet 博士是 Hollingsworth 博士指导下的研究助理教授， 是 RAP 项目的主任，也是该研究专家奖的申请人。 RAP计划 在 UNMC 的胰腺癌研究计划内运作，对胰腺癌很重要并且目前得到资助 部分由：胰腺癌 SPORE p50 资助；早期检测正在进行和待定的 U01 拨款 研究网络；作为肿瘤微环境网络一部分的 U54 资助；以及多项 R01 拨款 个别调查员。 Grandgenet 博士的一个关键作用是与合作者合作确定样本 研究项目所需的信息，根据需要修改我们的 SOP 以获得这些样本，然后 向调查人员提供这些样本。该资源的领导层必须是训练有素的人员 研究专家，通过参与尸检、全面的尸检而拥有丰富的经验 对可用患者样本的了解，以及在科学层面上与每个样本互动的能力 研究者。收集的标本包括原发肿瘤、残留未受影响的胰腺、远处转移瘤、所有 未受影响的组织以及血液和腹水等液体。这些组织和液体被快速收获 根据旨在维持组织细胞和分子完整性的 SOP。精选的新鲜样品是 立即处理或用于活细胞培养/异种移植程序。这些程序是在 并基于 UNMC 和其他机构资助项目的需求。目前，我们 将新鲜样本运送到其他实验室进行血液生物标志物（蛋白质、RNA、 DNA）、循环肿瘤细胞、包括类器官在内的细胞培养物以及其他过程。剩余样品 被冷冻，保存在长期储存设施中，并通过请求和合作提供 地方、国家和国际胰腺癌研究。 Grandgenet 博士在各个方面都发挥着重要作用 这些生物样本的收集、处理、注释和分发。