Critical Resources Provided by UNMC RAP Biorepository Stimulate Cancer Research

UNMC RAP 生物样本库提供的关键资源促进癌症研究

基本信息

批准号：
10493411
负责人：
Paul M Grandgenett
金额：
$ 14.47万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-15 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10493411
关键词：
Address Archives Ascites Autopsy Award Blood Cancer Center Cancer Patient Cancer Research Project Cessation of life Collaborations Collection DNA Disease Distant Metastasis Drug or chemical Tissue Distribution Focus Groups Freezing Funding Grant Hour Human Individual Institution International Knowledge Laboratories Leadership Liquid substance Malignant Neoplasms Malignant neoplasm of pancreas Malignant neoplasm of prostate Medical center Modeling Molecular Nebraska Neoplasm Circulating Cells Normal tissue morphology Oncology Organ Organoids Patients Play Postdoctoral Fellow Primary Neoplasm Process Prostate Proteins RNA Research Research Personnel Research Project Grants Research Training Residual state Resources Role Sampling Specialist Specimen TNFRSF5 gene Tissues Training Universities Work Xenograft procedure anticancer research base biobank blood-based biomarker design experience innovation mid-career faculty programs success volunteer

项目摘要

Project Summary The highly innovative Rapid Autopsy Program (RAP) addresses a major limitation in pancreatic and prostate cancer research: the lack of quality human specimens of cancer representing all stages of disease. This program, led by Dr. Paul M. Grandgenett, which includes rapid collection of large quantities of all organs and fluids within 5 hours of death, is a unique program in the world and is designed to maintain cellular and molecular integrity of the tissue. The RAP is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program and the GU Oncology Focus Group, that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreas or prostate cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center is Dr. Michael A. Hollingsworth, while the prostate group is led by Dr. Surinder Batra and Dr. Shawna Boyle. Dr. Grandgenett, a Research Associate Professor under Dr. Hollingsworth's aegis, is the director of the RAP and the applicant for this Research Specialist Award. The rapid autopsy program operates within the Pancreatic Cancer Research Program at UNMC and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending P01 and U01 grants and several R01 grants to individual investigators. Dr. Grandgenett personally directs the collection of primary tumor, residual unaffected tissues, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites for all PDAC and Prostate cancer patients. He has also established an extensive normal tissue acquisition program that is highly utilized. Dr. Grandgenett determines in advance how specific samples will be prioritized based on the needs of funded projects both at UNMC and at other institutions. Currently, Dr. Grandgenett provides fresh samples to multiple laboratories, at UNMC and outside-UNMC, conducting research on blood-based biomarkers (proteins, RNA, DNA), circulating tumor cells, patient-derived modeling including organoids and xenografts, and other processes. The remaining samples are snap frozen, maintained in long-term storage and made available through request and collaboration for local, national and international cancer studies. Key roles of Dr. Grandgenett is to coordinate the training and participation of the thirty RAP volunteers and to work with collaborators to determine the samples necessary for their research project, to modify our SOP as needed to obtain those samples, confirm specimen quality and then to distribute those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of patient sample availability, as well as the ability to interact on a scientific level with each investigator. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens and plays a vital role in expanding collaborative projects with investigators at UNMC and on a national and international level.

项目概要高度创新的快速尸检计划 (RAP) 解决了胰腺和前列腺的主要局限性癌症研究：缺乏代表疾病各个阶段的优质人类癌症标本。这该计划由 Paul M. Grandgenet 博士领导，其中包括快速收集大量所有器官和死亡后 5 小时内补充液体是世界上独一无二的计划，旨在维持细胞和组织的分子完整性。 RAP 是内布拉斯加大学医学中心的核心组成部分 (UNMC) 胰腺癌研究计划和 GU 肿瘤学焦点小组，其任务是收集、归档和分发最近去世的胰腺患者的组织和液体或前列腺癌。弗雷德和帕梅拉医院胰腺癌研究项目主任巴菲特癌症中心由 Michael A. Hollingsworth 博士领导，前列腺组由 Surinder Batra 博士领导，肖娜·博伊尔博士。 Grandgenet 博士是 Hollingsworth 博士主持下的研究副教授， RAP 主任和该研究专家奖的申请人。快速尸检程序开始运作 UNMC 胰腺癌研究计划的一部分，对以下机构很重要，目前部分资金来自：胰腺癌 SPORE p50 资助；正在进行和待定的 P01 和 U01 赠款以及几项 R01 赠款个别调查员。 Grandgenet博士亲自指导采集原发肿瘤，残余未受影响组织、远处转移、所有未受影响的组织以及所有 PDAC 和腹水等液体前列腺癌患者。他还建立了广泛的正常组织采集计划，该计划非常有效被利用。 Grandgenet 博士根据需求提前确定具体样品的优先顺序资助了 UNMC 和其他机构的项目。目前，Grandgenet 博士向 UNMC 内和 UNMC 外的多个实验室对基于血液的生物标志物（蛋白质、 RNA、DNA）、循环肿瘤细胞、患者衍生模型（包括类器官和异种移植物）以及其他流程。剩余的样品被快速冷冻，长期储存并可供使用通过地方、国家和国际癌症研究的请求和合作。博士的关键角色 Grandgenett 将协调 30 名 RAP 志愿者的培训和参与，并与合作者确定其研究项目所需的样本，根据需要修改我们的 SOP 获取这些样本，确认样本质量，然后将这些样本分发给研究人员。这是对于该资源的领导者来说，成为一名训练有素、具有广泛知识的研究专家至关重要。通过参与尸检获得的经验，对患者样本可用性的全面了解，以及与每个研究者进行科学层面互动的能力。 Grandgenet 博士在以下方面发挥了重要作用：这些生物样本的收集、处理、注释和分发的各个方面都发挥着至关重要的作用在扩大与 UNMC 的调查人员以及国家和国际层面的合作项目方面发挥作用。