Harvesting specific plant metabolites from hairy root cultures using magnetized n

使用磁化n从毛状根培养物中收获特定的植物代谢物

基本信息

批准号：
8712853
负责人：
JOHN M. LITTLETON
金额：
$ 18.98万
依托单位：
NAPROGENIX, INC
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8712853
关键词：
Address Area Binding Biological Factors Biotechnology Cell Culture Techniques Cell Survival Cells Characteristics Chemical Engineering Chemicals Collaborations Complex Mixtures Devices Drug Industry Endocytosis Engineering Exposure to Faculty Fermentation Flavonoids Funding Genetically Modified Plants Harvest High Pressure Liquid Chromatography Individual Industry Kentucky Licensing Life Mammalian Cell Marketing Measures Medicine Methods Nutrient One-Step dentin bonding system Pharmacologic Substance Phase Plant Roots Plants Preparation Process Production Protocols documentation Quercetin Research Research Personnel Schedule Silicon Dioxide Small Business Technology Transfer Research Solidago Specificity Surface System Taxoids Technology Testing Time Tissues Universities Vinca Alkaloids chemotherapeutic agent commercialization cytotoxicity design extracellular improved in vitro testing magnetic field microbial mutant nanoparticle novel prototype public health relevance radioligand response tissue culture

项目摘要

DESCRIPTION: Plants produce a wide variety of valuable bioactive metabolites, but these are commonly present in low concentrations in the wild-type plant. This makes the separation and purification of these compounds complicated and expensive. The applicant company, Naprogenix Inc, has developed a technology for increasing the yields of specific bioactive metabolites in mutant plant cell cultures, which promises to lessen this problem. However, the value of this, or any other approach using genetically-modified plant cells, is limited by the necessity of the wasteful destruction of the plant cells in order to extract the required products. The process would be more efficient if products could be harvested from cells in continuous culture. Nanoparticles are taken up by cells by endocytosis, and subsequently exocytosed. They can also be engineered to adsorb specific chemicals. This suggests that specifically-engineered (functionalized and magnetized) nanoparticles could be used to repeatedly "harvest" specific metabolites from living plant cell cultures. One of the company's major projects is designed to generate novel flavonoids in goldenrod hairy root cultures. The applicants have shown that functionalized silica nanoparticles adsorb similar flavonoids and that they are taken up and removed from these plant cell cultures without compromising the viability of the cultures. The objective is now to engineer optimally-functionalized magnetized silica nanoparticles (by collaborators at the University of Kentucky) to harvest specific flavonoids from these hairy roots. The specific aims of phase I are to show that (a) exposure to these nanoparticles translocate flavonoids from the intracellular compartment of hairy roots to the extracellular medium (b) these extracellular magnetized nanoparticles can be concentrated in a magnetic field (c) significant amounts of flavonoids can be eluted and collected from these nanoparticles following their passage through plant cells. If this is successful, then, in phase II, the applicants will design ad test nanoparticles for harvesting other bioactive metabolites from optimized plant cell cultures. These will include very high value chemotherapeutic agents, such as the taxoids and vinca alkaloids. If successful this approach should make a major impact on the use of plant cell cultures for the production and isolation of high value natural products. The nanoparticles, and the harvesting technologies as applied to plant cells, are patentable, and have major commercial implications for the applicant company and the University.

描述：植物产生多种有价值的生物活性代谢物，但这些代谢物在野生型植物中的浓度通常较低。这使得这些化合物的分离和纯化变得复杂且昂贵。申请公司 Naprogenix Inc 开发了一种技术，可提高突变植物细胞培养物中特定生物活性代谢物的产量，有望缓解这一问题。然而，这种方法或使用转基因植物细胞的任何其他方法的价值都受到为了提取所需产品而浪费地破坏植物细胞的必要性的限制。如果可以从连续培养的细胞中收获产物，该过程将会更加有效。纳米颗粒通过胞吞作用被细胞摄取，随后被胞吐。它们还可以被设计为吸附特定的化学物质。这表明专门设计的（功能化和磁化）纳米颗粒可用于从活植物细胞培养物中重复“收获”特定代谢物。该公司的主要项目之一旨在在黄花毛状根培养物中产生新型黄酮类化合物。申请人已经表明，功能化二氧化硅纳米粒子吸附类似的黄酮类化合物，并且它们被从这些植物细胞培养物中吸收和去除，而不损害培养物的活力。现在的目标是设计最佳功能化的磁化二氧化硅纳米粒子（由肯塔基大学的合作者），以从这些毛状根中收获特定的黄酮类化合物。第一阶段的具体目标是证明（a）暴露于这些纳米颗粒会将黄酮类化合物从毛根的细胞内区室转移到细胞外介质（b）这些细胞外磁化纳米颗粒可以集中在磁场中（c）大量的在这些纳米粒子通过植物细胞后，可以从这些纳米粒子中洗脱并收集类黄酮。如果成功，那么在第二阶段，申请人将设计广告测试纳米颗粒，用于从优化的植物细胞培养物中收获其他生物活性代谢物。这些将包括非常高价值的化疗药物，例如紫杉烷和长春花生物碱。如果成功，这种方法将对植物细胞培养物用于生产和分离高价值天然产品产生重大影响。纳米粒子以及应用于植物细胞的收获技术是可申请专利的，并且对申请公司和大学具有重大的商业意义。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nanoharvesting of bioactive materials from living plant cultures using engineered silica nanoparticles.

使用工程二氧化硅纳米颗粒从活植物培养物中纳米收获生物活性材料。

DOI：
10.1016/j.msec.2019.110190
发表时间：
2020-01-01
期刊：
Materials science & engineering. C, Materials for biological applications
影响因子：
0
作者：
M. Khan;W. T. Wallace;Jatinder Sambi;D. Rogers;J. Littleton;S. Rankin;B. L. Knutson
通讯作者：
B. L. Knutson

Strategy for Conjugating Oligopeptides to Mesoporous Silica Nanoparticles Using Diazirine-Based Heterobifunctional Linkers.

使用基于二氮丙啶的异双功能连接体将寡肽缀合至介孔二氧化硅纳米粒子的策略。

DOI：
发表时间：
2022-02-11
期刊：
影响因子：
0
作者：
Khan, Md Arif;Ghanim, Ramy W;Kiser, Maelyn R;Moradipour, Mahsa;Rogers, Dennis T;Littleton, John M;Bradley, Luke H;Lynn, Bert C;Rankin, Stephen E;Knutson, Barbara L
通讯作者：
Knutson, Barbara L

Mechanism of Mesoporous Silica Nanoparticle Interaction with Hairy Root Cultures during Nanoharvesting of Biomolecules.

生物分子纳米收获过程中介孔二氧化硅纳米粒子与毛状根培养物相互作用的机制。

DOI：
发表时间：
2021
期刊：
影响因子：
3.7
作者：
Khan, Md Arif;Fugate, Madeleine;Rogers, Dennis T;Sambi, Jatinder;Littleton, John M;Rankin, Stephen E;Knutson, Barbara L
通讯作者：
Knutson, Barbara L