Generation of a Complete Set of Precise Null Bar-Coded Deletion Mutants of Mycobacterium tuberculosis

结核分枝杆菌一整套精确空条形码缺失突变体的生成

• 批准号: 9754771
• 负责人: WILLIAM Robert JACOBS
• 金额: $ 57.21万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-14 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9754771
• 关键词: Alleles; Attenuated; Bacillus (bacterium); Bacteriophages; Bar Codes; Biological Markers; Biology; Cessation of life; Cloning; Collection; Communities; Complement; Containment; DNA; DNA Sequence; Data; Databases; Drug Targeting; Electroporation; Engineering; Epidemic; Escherichia coli; Evaluation; Extreme drug resistant tuberculosis; Foundations; Freeze Drying; Frequencies; Gene Deletion; Gene Targeting; Generations; Genes; Genome; Genotype; Glycerol; Goals; Gold; Growth; HIV; In Vitro; International; Interruption; Laboratories; Libraries; Metabolism; Methodology; Modeling; Molecular Genetics; Morbidity - disease rate; Multidrug-Resistant Tuberculosis; Mus; Mycobacteriophages; Mycobacterium smegmatis; Mycobacterium tuberculosis; Nucleotides; Online Systems; Phenotype; Physiology; Plasmid Cloning Vector; Plasmids; Production; Proteins; Protocols documentation; Quality Control; Reagent; Recombinants; Research; Resources; Technology; Tuberculosis; Vaccines; Virulent; base; chemotherapy; cost effective; fitness; genome-wide; global health; knockout gene; mortality; mutant; nonhuman primate; pathogen; reverse genetics; tuberculosis diagnostics; vector

Abstract Tuberculosis (TB) continues to be a major global health problem with 9 million new cases per year resulting in 1.7 million deaths [1], due in large part to the HIV epidemic and emergence of multi- and extensively drug resistant TB strains (MDR- and XDR-TB) that have confounded TB control. There will be no lasting reduction of TB morbidity and mortality without rapid and cost-effective TB diagnostics, efficacious vaccines, and shortened, well-tolerated chemotherapy, which can only be developed on the foundation of understanding the biology of the tubercle bacilli and subsequent host-pathogen interactions. The goal of this proposal is to accelerate TB research through the completion and distribution of these valuable precise null deletion M. tuberculosis gene knockout resources to the international TB research community. We will partner with BEI for distribution of reagents.

抽象的 结核病 (TB) 仍然是全球主要健康问题，新增病例达 900 万例 每年导致 170 万人死亡[1]，这在很大程度上是由于艾滋病毒的流行和 多重耐药和广泛耐药结核菌株（MDR-和XDR-TB）的出现 混淆了结核病控制。结核病发病率不会持久降低 如果没有快速且具有成本效益的结核病诊断、有效的疫苗和 缩短时间、耐受性良好的化疗，只能在此基础上开发 了解结核杆菌和随后的宿主病原体的生物学 互动。该提案的目标是通过完成以下工作来加速结核病研究 这些有价值的精确零缺失结核分枝杆菌基因敲除的分布和分布 向国际结核病研究界提供资源。我们将与 BEI 合作 试剂的分配。