Generation of a Complete Set of Precise Null Bar-Coded Deletion Mutants of Mycobacterium tuberculosis

结核分枝杆菌一整套精确空条形码缺失突变体的生成

• 批准号: 9754771
• 负责人: WILLIAM Robert JACOBS
• 金额: $ 57.21万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-14 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9754771
• 关键词: Alleles; Attenuated; Bacillus (bacterium); Bacteriophages; Bar Codes; Biological Markers; Biology; Cessation of life; Cloning; Collection; Communities; Complement; Containment; DNA; DNA Sequence; Data; Databases; Drug Targeting; Electroporation; Engineering; Epidemic; Escherichia coli; Evaluation; Extreme drug resistant tuberculosis; Foundations; Freeze Drying; Frequencies; Gene Deletion; Gene Targeting; Generations; Genes; Genome; Genotype; Glycerol; Goals; Gold; Growth; HIV; In Vitro; International; Interruption; Laboratories; Libraries; Metabolism; Methodology; Modeling; Molecular Genetics; Morbidity - disease rate; Multidrug-Resistant Tuberculosis; Mus; Mycobacteriophages; Mycobacterium smegmatis; Mycobacterium tuberculosis; Nucleotides; Online Systems; Phenotype; Physiology; Plasmid Cloning Vector; Plasmids; Production; Proteins; Protocols documentation; Quality Control; Reagent; Recombinants; Research; Resources; Technology; Tuberculosis; Vaccines; Virulent; base; chemotherapy; cost effective; fitness; genome-wide; global health; knockout gene; mortality; mutant; nonhuman primate; pathogen; reverse genetics; tuberculosis diagnostics; vector

Abstract Tuberculosis (TB) continues to be a major global health problem with 9 million new cases per year resulting in 1.7 million deaths [1], due in large part to the HIV epidemic and emergence of multi- and extensively drug resistant TB strains (MDR- and XDR-TB) that have confounded TB control. There will be no lasting reduction of TB morbidity and mortality without rapid and cost-effective TB diagnostics, efficacious vaccines, and shortened, well-tolerated chemotherapy, which can only be developed on the foundation of understanding the biology of the tubercle bacilli and subsequent host-pathogen interactions. The goal of this proposal is to accelerate TB research through the completion and distribution of these valuable precise null deletion M. tuberculosis gene knockout resources to the international TB research community. We will partner with BEI for distribution of reagents.

抽象的 结核病（TB）仍然是全球重大健康问题，有900万例新病例 每年导致170万人死亡[1]，这在很大程度上归因于艾滋病毒的流行和 多种和广泛的抗药性结核病菌株（MDR-和XDR-TB）的出现 有混淆结核病控制。 TB发病率不会持久降低 死亡率没有快速且具有成本效益的结核病诊断，有效的疫苗和 缩短，耐受良好的化学疗法，只能在基础上开发 了解结核细菌的生物学和随后的宿主病原 互动。该建议的目的是通过完成加速结核病研究 和分布这些有价值的精确零缺失。结核病基因敲除 国际结核病研究界的资源。我们将与Bei合作 试剂的分布。