Generation of a Complete Set of Precise Null Bar-Coded Deletion Mutants of Mycobacterium tuberculosis

结核分枝杆菌一整套精确空条形码缺失突变体的生成

基本信息

批准号：
9417850
负责人：
WILLIAM Robert JACOBS
金额：
$ 56.19万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-14 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9417850
关键词：
Alleles Attenuated Bacillus (bacterium)Bacteriophages Bar Codes Biological Markers Biology Cessation of life Cloning Collection Communities Complement Containment DNA DNA Sequence Data Databases Diagnostic Drug Targeting Electroporation Engineering Epidemic Escherichia coli Evaluation Extreme drug resistant tuberculosis Foundations Freeze Drying Frequencies Gene Deletion Gene Targeting Generations Genes Genome Genotype Glycerol Goals Gold Growth HIV In Vitro International Interruption Laboratories Libraries Metabolism Methodology Modeling Molecular Genetics Morbidity - disease rate Mus Mycobacteriophages Mycobacterium smegmatis Mycobacterium tuberculosis Nucleotides Online Systems Phenotype Physiology Plasmid Cloning Vector Plasmids Production Proteins Protocols documentation Quality Control Reagent Recombinants Research Resources Technology Tuberculosis Vaccines Virulent base chemotherapy cost effective extensive drug resistance fitness genome-wide global health knockout gene mortality mutant nonhuman primate pathogen reverse genetics vector

项目摘要

Abstract Tuberculosis (TB) continues to be a major global health problem with 9 million new cases per year resulting in 1.7 million deaths [1], due in large part to the HIV epidemic and emergence of multi- and extensively drug resistant TB strains (MDR- and XDR-TB) that have confounded TB control. There will be no lasting reduction of TB morbidity and mortality without rapid and cost-effective TB diagnostics, efficacious vaccines, and shortened, well-tolerated chemotherapy, which can only be developed on the foundation of understanding the biology of the tubercle bacilli and subsequent host-pathogen interactions. The goal of this proposal is to accelerate TB research through the completion and distribution of these valuable precise null deletion M. tuberculosis gene knockout resources to the international TB research community. We will partner with BEI for distribution of reagents.

抽象的结核病（TB）仍然是全球重大健康问题，有900万例新病例每年导致170万人死亡[1]，这在很大程度上归因于艾滋病毒的流行和多种和广泛的抗药性结核病菌株（MDR-和XDR-TB）的出现有混淆结核病控制。 TB发病率不会持久降低死亡率没有快速且具有成本效益的结核病诊断，有效的疫苗和缩短，耐受良好的化学疗法，只能在基础上开发了解结核细菌的生物学和随后的宿主病原互动。该建议的目的是通过完成加速结核病研究和分布这些有价值的精确零缺失。结核病基因敲除国际结核病研究界的资源。我们将与Bei合作试剂的分布。