A chemical-genetic approach to decipher the function of corticothalamic feedback

破译皮质丘脑反馈功能的化学遗传学方法

• 批准号: 8610288
• 负责人: Daniel B. Polley
• 金额: $ 20.5万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8610288
• 关键词: Acoustics; Action Potentials; Affect; Animals; Area; Auditory; Auditory area; Axon; Behavior; Behavioral; Blood - brain barrier anatomy; Brain; Cerebral cortex; Cholinergic Receptors; Chronic; Cortical Column; Data; Designer Drugs; Dorsal; Dose; Dreams; Drug Receptors; Engineering; Epilepsy; Feedback; Frequencies; Hearing; Hour; Implant; Individual; Injection of therapeutic agent; Lead; Learning; Lesion; Link; Literature; Measures; Medial; Medial geniculate body; Mediating; Medical Technology; Membrane; Memory; Morphology; Mus; Neuraxis; Neurons; Parkinson Disease; Pathway interactions; Perception; Pharmaceutical Preparations; Phase; Physiology; Presynaptic Terminals; Process; Reporting; Research; Role; Sensory; Slice; Specificity; Stream; Synapses; System; Techniques; Technology; Testing; Thalamic structure; Time; Tinnitus; Transgenic Mice; Unconscious State; Viral Vector; Work; auditory stimulus; awake; base; cell type; chemical genetics; cognitive function; corticofugal system; design; dosage; extracellular; in vivo; insight; loss of function; nervous system disorder; next generation; novel strategies; predictive modeling; public health relevance; receptive field; receptor; relating to nervous system; research study; response; sound; synthetic drug; tool; vigilance; vocalization

DESCRIPTION (provided by applicant: Sensory representations are dynamically maintained by ascending and descending connections linking the cerebral cortex and the thalamus. Although the overall extent and topographic specificity of descending corticothalamic projections can equal or surpass that of thalamocortical projections, little is known about their role in perception or learning. Here, we propose a new chemical-genetic approach known as DREADDs (Designer Receptors Exclusively Activated by Designer Drugs) to parse the functional contribution of corticothalamic circuits that arise from layer 5 versus layer 6 of primay and secondary areas of the auditory cortex. By silencing specific cortical feedback circuits while recording from ensembles of thalamic neurons in mice that are either passively listening to sound or are actively engaged in listening tasks, we will gain deeper insight into the workings of multiple, parallel feedback systems that enable the cerebral cortex to modify its afferent input stream.

描述（由申请人提供：感觉表征是通过连接大脑皮层和丘脑的上升和下降连接动态维持的。尽管下降皮质丘脑投射的总体范围和地形特异性可以等于或超过丘脑皮质投射，但对其作用知之甚少在这里，我们提出了一种新的化学遗传方法，称为 DREADD（由设计药物独家激活的设计受体）来解析功能贡献。通过沉默特定的皮质反馈回路，同时记录被动听声音或主动参与聆听任务的小鼠丘脑神经元群，研究由听觉皮层的第 5 层与第 6 层产生的皮质丘脑回路的差异。 ，我们将更深入地了解多个并行反馈系统的工作原理，这些系统使大脑皮层能够修改其传入输入流。

项目成果

A Corticothalamic Circuit for Dynamic Switching between Feature Detection and Discrimination.

用于特征检测和辨别之间动态切换的皮质丘脑电路。

• 发表时间: 2017-07-05
• 影响因子: 16.2
• 作者: Guo, Wei;Clause, Amanda R;Barth;Polley, Daniel B
• 通讯作者: Polley, Daniel B