On-the-Fly Field-potential Sensing Electrode Track based NSC sorting for brain re

基于动态场电位传感电极轨迹的 NSC 脑再分类

基本信息

批准号：
8712598
负责人：
John Collins
金额：
$ 35万
依托单位：
BIOPICO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-05 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8712598
关键词：
Affect Algorithms Alzheimer&apos s Disease American Amplifiers Amyotrophic Lateral Sclerosis Area Arizona Asses Biological Assay Brain California Cell Separation Cell Signaling Process Cell Therapy Cell model Cells Cellular Morphology Clinical Clinical Laboratory Techniques Complex Computer software County Databases Derivation procedure Detection Development Differentiation Antigens Direct Costs Disease Electrodes Family Feasibility Studies Fluorescent Dyes Gene Expression Genetic Genomics Geometry Glass Goals Health Height Human Huntington Disease Individual Label Laboratories Lead Lesion Manuals Measurement Mental disorders Methods Metric Microelectrodes Microfluidics Microscope Modification Morphologic artifacts Neuraxis Neurodegenerative Disorders Neurons Neurosciences Noise Optics Oranges Organ Parkinson Disease Patients Pediatric Hospitals Performance Phase Pluripotent Stem Cells Polymers Productivity Protocols documentation Pump Quantitative Reverse Transcriptase PCR Regenerative Medicine Replacement Therapy Research Research Institute Research Personnel Response to stimulus physiology Risk Sampling Signal Transduction Somatic Cell Sorting - Cell Movement Specificity Spinal cord injury Stem cells Sterility Stimulus Stroke System Techniques Technology Teratoma Therapeutic Time Tissues Translating Translations Undifferentiated Universities Validation Width abstracting base brain research brain tissue cell type clinical application cost design electric impedance epigenetic marker fluorexon fly human disease improved induced pluripotent stem cell nanosystems nerve stem cell nervous system disorder novel pluripotency prevent programs prototype public health relevance remyelination research study response simulation stem cell biology tool verification and validation

项目摘要

DESCRIPTION (provided by applicant): "'On-the-Fly Field-potential Sensing Electrode Track' (OFFSET) based NSC sorting for brain research". Abstract: The past decade has acquired a profound paradigm shift in high throughput tools for brain research with the advent of programming techniques in micro/nano systems, stem cells, signal processing and genomics. One area in which these technologies have opened new avenues has been the application of pluripotent stem cells to study neurodegenerative diseases. The derivation of patient-specific reprogrammed somatic cells enables immunologically compatible viable brain tissue for use in studying disease development, creating therapies for neurological disorders and developing perfect models for the cells of the central nervous system that are harmed in the diseases. The major challenge in translating stem cell biology into such useful tissue is the establishment of effective separation methods to isolate differentiated cells and exclude cells that hinder graft performance or lead to teratoma formation. Unfortunately, conventional separation techniques for stem cells such as microscope-assisted manual isolation, FACS and MACS require intensive labor, exogenous labeling or genetic modification is not suitable for such clinical applications. Therefore, Biopico Systems teams with the researchers from University of California, Irvine for their expertise in microfluidics, the Children's Hospital of Orange County (CHOC) Research Institute for their expertise in iPS cells therapy, and Arizona State University for their expertis in neuronal stimulus recording using microelectrode array in order to demonstrate the technical feasibility of a label-free electrical field potential marker based cell sorting for brain research With our understanding of the practical constraints of the system components from the laboratories of our collaborators, we propose to develop complex therapeutic technology for research and practice. We envision developing a family of systems to sort neural stem cells for several applications to lead technological transformation in neuroscience.

描述（由申请人提供）：“用于大脑研究的基于‘动态场电位传感电极轨迹’（OFFSET）的 NSC 分类”。摘要：过去十年，随着微/纳米系统、干细胞、信号处理和基因组学编程技术的出现，大脑研究的高通量工具发生了深刻的范式转变。这些技术开辟了新途径的一个领域是应用多能干细胞来研究神经退行性疾病。患者特异性重编程体细胞的衍生使得免疫相容的活脑组织可用于研究疾病发展、创造神经系统疾病的疗法以及为在疾病中受损的中枢神经系统细胞开发完美的模型。将干细胞生物学转化为此类有用组织的主要挑战是建立有效的分离方法来分离分化细胞并排除阻碍移植性能或导致畸胎瘤形成的细胞。不幸的是，传统的干细胞分离技术，如显微镜辅助手动分离、FACS和MACS需要大量劳动力、外源标记或基因修饰，不适合此类临床应用。因此，Biopico Systems 与来自加州大学欧文分校的研究人员在微流体方面的专业知识、橙县儿童医院 (CHOC) 研究所在 iPS 细胞治疗方面的专业知识以及亚利桑那州立大学在神经元刺激记录方面的专业知识进行了合作使用微电极阵列来证明基于无标记电场电位标记的细胞分选用于大脑研究的技术可行性随着我们对合作者实验室系统组件的实际限制的了解，我们建议开发复杂的治疗方法用于研究和实践的技术。我们设想开发一系列系统来对神经干细胞进行分类，以用于多种应用，从而引领神经科学的技术变革。