Pathology and Genomics Core

病理学和基因组学核心

• 批准号: 9404836
• 负责人: Jian-Jun Wei
• 金额: $ 9.91万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9404836
• 关键词: AIDS prevention; Acquired Immunodeficiency Syndrome; Adult; Advanced Malignant Neoplasm; Africa; American; Area; Biological; Biological Markers; Blood; Cancer Diagnostics; Cervical; Clinical; Clinical Data; Collaborations; Communicable Diseases; Communities; Country; Coupled; DNA; DNA Methylation; DNA sequencing; Databases; Development Plans; Diagnostic; Educational Curriculum; Emergency Situation; Epigenetic Process; Foundations; Funding; Future; Genes; Genetic Research; Genomics; Goals; HIV; Health; Healthcare; Hepatitis B; Hepatitis C; Histology; Human Papillomavirus; Immunohistochemistry; Income; Institutes; Institution; International; International AIDS; Laboratories; Leadership; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Medical; Medical Education; Medical Research; Methods; Methylation; Modernization; Molecular Analysis; Nigeria; Nigerian; Pathology; Patients; Positioning Attribute; Preparation; Prevention; Prevention Research; Primary carcinoma of the liver cells; Procedures; Recording of previous events; Research; Research Infrastructure; Research Personnel; Research Training; Resources; Sampling; Services; Specimen; System; Technology; Telepathology; Time; Tissues; Training Activity; Training Programs; Training Support; United States National Institutes of Health; Universities; Update; Urine; Viral hepatitis; Virus; Virus Diseases; anticancer research; bead chip; biomedical informatics; cancer care; cancer genetics; career; community organizations; data sharing; digital; disorder prevention; epigenomics; global health; improved; infrastructure development; inter-institutional; interdisciplinary approach; interest; international center; medical schools; operation; programs; repository; response; statistics; tool; whole genome

Project Summary We propose to establish a Consortium to study “Epigenomic Biomarkers of HIV-Associated Cancers in Nigeria.” Our research focus and core services development plan has been prioritized by our Nigerian partners at University of Jos (UniJos), Plateau State, Nigeria located in the center of the country and University of Lagos (UniLag), Lagos State in the densely populated southwest. Our focus on the epigenetics of HIV-associated hepatocellular carcinoma (HCC) and cervical cancer is justified by our extensive collaborative research history in viral diseases including HIV, human papilloma virus, hepatitis B and C viruses. Northwestern University and Mayo Medical School will serve as the high-income American partner institutions which is justified by the long history of research and research training collaboration with the Nigerian institutions coupled with its expertise in global health and ample complementary resources. The specific aims of the Pathology and Genomics Core (PGC) are: 1. to enhance the existing laboratory systems to develop high-quality services in telepathology, immunohistochemistry (IHC), cancer biospecimen repository, and molecular analyses at UniJos and UniLag, 2. to develop a collaborative pathology and genomics laboratory team focused on providing core services for HIV- associated cancer genetics research in Nigeria, and 3. to provide oversight and support for the pathology and genomics needs of Projects 1 (HCC) and 2 (cervical) proposed under this U54. Our PGC will provide investigators from UniJos and UniLag with high quality patient-derived biospecimens and the laboratory tools to advance cancer research. To accomplish this, our PGC will maintain, update, and incorporate new standard operating procedures, laboratory methods and technologies. Our PGC will be functionally divided into three divisions/units: 1. Pathology Diagnostics: this includes all related tasks of routine histology, IHC and digital telepathology, 2. Biospecimen Repository: this includes tissue and biospecimen procurement, detailed sample annotation and well-controlled biospecimen banking procedures, and 3. Genomic Analysis: this includes DNA extraction/preparation, qPCR for specific gene sequences, whole genome and epigenetic sequencing for DNA methylation using Illumina Infinium Methylation EPIC BeadChip. The proposed PGC will utilize a strong interdisciplinary approach involving several U54 investigators who represent a broad range of research interests/expertise across UniJos, UniLag, Northwestern and Mayo. The overall goal of the PGC will be to support and facilitate research proposed in Projects 1 (HCC) and 2 (cervical), provide facilities for training activities under the Career Enhancement Core (CEC), coordination of research services with the Biomedical Informatics and Statistics Core (BISC), build infrastructure and research management with the Administrative Core (AC).

项目摘要 我们建议建立一个联盟，以研究“尼日利亚与HIV相关癌症的表观基因组生物标志物”。 我们的研究重点和核心服务开发计划已由我们的尼日利亚合作伙伴优先考虑 乔斯大学（UNIJOS），尼日利亚高原州，位于该国中心和拉各斯大学 （unilag），拉各斯州位于人口稠密的西南。我们关注与HIV相关的表观遗传学 我们广泛的合作研究历史证明了肝细胞癌（HCC）和宫颈癌是合理的 在病毒疾病中，包括HIV，人乳头瘤病毒，乙型肝炎和C病毒。西北大学和 梅奥医学院将担任高收入美国合作伙伴机构 研究与研究培训的历史与尼日利亚机构合作，并在其方面的专业知识 全球健康和充足的互补资源。病理和基因组核心的具体目的 （PGC）是：1。为了增强现有的实验室系统，以开发心脏病学高质量服务， 免疫组织化学（IHC），癌症生物环体存储库和Unijos and Unilag的分子分析，2。 开发一个协作病理学和基因组学实验室团队，致力于为HIV提供核心服务 尼日利亚相关的癌症遗传学研究，3。 根据U54提出的项目1（HCC）和2（宫颈）的基因组需求。我们的PGC将提供 Unijos和Unilag的调查人员具有高质量的患者衍生的生物测量和实验室工具 预先癌症研究。为此，我们的PGC将维护，更新和合并新标准 操作程序，实验室方法和技术。我们的PGC将在功能上分为三个 部门/单位：1。病理学诊断：这包括所有常规组织学，IHC和数字的所有相关任务 远程病理学，2。生物传播存储库：这包括组织和生物循环采购，详细的样品 注释和控制良好的生物测量银行程序和3。基因组分析：这包括DNA 提取/制备，特定基因序列的qPCR，整个基因组和表观遗传测序DNA 使用Illumina Infinium甲基化史诗般的Beadchip甲基化。拟议的PGC将利用强度 跨学科方法涉及几位代表广泛研究的U54调查人员 Unijos，Unilag，Northwestern和Mayo的利益/专业知识。 PGC的总体目标将是 支持和促进项目1（HCC）和2（宫颈）中提出的研究提供培训的设施 职业增强核心（CEC）下的活动，研究服务与生物医学的协调 信息学和统计核心（BISC），通过行政管理建立基础架构和研究管理 核心（AC）。