The Influence of Capsule Composition on Lens Biology

胶囊成分对晶状体生物学的影响

• 批准号: 9242641
• 负责人: MELINDA K DUNCAN
• 金额: $ 39万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9242641
• 关键词: Adverse effects; Basement membrane; Biology; Cataract; Cataract Extraction; Cells; Cicatrix; Communication; Data; Development; Embryo; Epithelial; Epithelial Cells; Epithelium; Excision; Extracapsular; Extracellular Matrix; Extracellular Matrix Proteins; Eye; Fibronectins; Gene Expression; Grant; Integrins; Lens Fiber; Lens development; Ligands; Mediating; Molecular; Molecular Target; Myofibroblast; Operative Surgical Procedures; Pathogenesis; Pathway interactions; Patient-Focused Outcomes; Phenotype; Play; Prevention; Process; Proteins; Regulation; Role; Signal Transduction; Surface; Testing; Tissues; Vesicle; Visual; Work; capsule; cell behavior; epithelial to mesenchymal transition; fiber cell; insight; lens; lens capsule; premature; prevent; public health relevance; transdifferentiation

DESCRIPTION (provided by applicant): The lens capsule is a thickened basement membrane surrounding the lens which provides essential structural support for lens cells and mediates connection between the lens and the remainder of the eye. Lens capsule components also serve as survival, proliferation and differentiation signals for the attached lens cells although te relative contribution of each extracellular matrix protein found in the lens capsule to lens cell behavior is mostly uncharacterized.. Integrins are major molecules mediating the communication between cells and their extracellular matrix. During the prior grant cycle we discovered that various integrins play essential roles in lens development as well as the pathogenesis of posterior capsular opacification (PCO), the major side effect of surgical cataract removal. However, the mechanisms by which integrins control lens development and the pathogenesis of PCO is not well understood. This application proposes to investigate the molecular mechanisms by which integrins control the fate decisions which regulate transitions from lens epithelial cells to fiber cells during development and "pearl type" PCO as well as transitions between epithelial cells and myofibroblasts during the process of fibrotic PCO development.

描述（由申请人提供）：镜头胶囊是透镜周围的地下膜增厚，可为晶状体细胞提供必不可少的结构支撑，并介导镜头和其余眼睛之间的连接。透镜胶囊成分还可以作为附着的晶状体细胞的生存，增殖和分化信号，尽管在晶状体囊中发现的每个细胞外基质蛋白的TE相对贡献对晶状体细胞行为的相对贡献主要是未经表征的。整联蛋白是介导细胞和细胞之间的通信之间的主要分子它们的细胞外基质。在先前的赠款周期中，我们发现各种整联蛋白在晶状体发育以及后囊膜无情中的发病机理（PCO）中起着重要作用，这是手术性白内障的主要副作用。但是，整合素控制晶状体发育和PCO发病机理的机制尚不清楚。该应用建议研究整联蛋白控制命运决策的分子机制，这些决策在发育过程中调节了从晶状体上皮细胞向纤维细胞的过渡，以及“ Pearl型” PCO，以及在纤维化PCO发育过程中上皮细胞和肌纤维细胞之间的过渡。