Animal Phenotyping (Core B)

动物表型分析（核心 B）

基本信息

批准号：
9259937
负责人：
GARY J SCHWARTZ
金额：
$ 23.55万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-01 至 2021-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9259937
关键词：
Adipocytes Adipose tissue Affect Anatomy Animals Autonomic Denervation Biology Body Composition Body Temperature Body Weight Brown Fat Bypass Calorimetry Carbon Dioxide Cell Separation Characteristics Complement Consumption Data Development Diabetes Mellitus Energy Intake Energy Metabolism Equipment Fatty acid glycerol esters Feeding behaviors Functional disorder Funding Generations Genetic Grant Histologic Human Immunologics Individual Laboratories Liver Magnetic Resonance Imaging Maintenance Manuscripts Measurement Measures Medicine Metabolic Metabolic Diseases Metabolism Microscope Mission Molecular Molecular Biology Molecular Genetics Morphology Mus Neuraxis New York Nutritional Study Obesity Operative Surgical Procedures Oxygen Phenotype Physical activity Physiology Procedures Process Publications Rattus Regulation Research Research Personnel Resources Rodent Rodent Model Running Scientist Services System Technical Expertise Temperature Thermogenesis Thinness Tissue Transplantation Tissues United States National Institutes of Health Universities Variant Viral Weight Weight Gain Work adipocyte differentiation animal resource base college cost cost effective design energy balance equipment acquisition fluorescence microscope gastrointestinal glucose metabolism insight lipid metabolism macrophage member metabolic phenotype microCT mouse model nutrition operation optogenetics prevent protein metabolism respiratory stem tool

项目摘要

Animal Phenotyping Core PROJECT SUMMARY/ABSTRACT Rodent models of obesity have provide invaluable to the study of obesity and to the work carried out by NYONRC investigators. To meet the growing needs of Center researchers, the proposed NYONRC Animal Phenotyping Core (APC) reflects a significant expansion of the Animal Energy Balance Core (Einstein) and integration with the animal component of the Adipose Tissue Core (Columbia). During the past 4 years of the current cycle, these components of the NYONRC have processed more than 14,000 service requests for 38 NYONRC members supported by 45 grants, and provided additional services to 9 NIH-funded non-NYONRC members. The services offered by the APC have been instrumental in obtaining 19 new or renewed grants, and in the generation of 213 publications. The proposed APC concentrates and coordinates significantly expanded technical and research capabilities of this amalgamated Core. The incorporation of additional animal resources at both the Einstein and Columbia campuses, will significantly increase the user and research base of the NYONRC overall, and of the APC Core in particular. By coordinated application of NYONRC dedicated facilities, and resources and expertise at both Columbia and Einstein, the proposed APC Core will combine sophisticated qualitative and quantitative measurements of ingestive behavior with concurrent assessments of energy expenditure, physical activity, body temperature, and repeatable noninvasive body composition analysis by MRI and MicroCT. Also available are a range of metabolic surgeries, including gastrointestinal bypass, autonomic denervation procedures, and adipose tissue services including: 1) quantifying adipocyte subpopulations (brown, white, beige), in terms of size, variation, and cellular ultrastructure; 2) primary cell isolation and analysis; 3) differentiation of adipocytes and adipose tissue macrophages; and 4) adipose tissue transplantation. This expanded array of services stems from the growing realization that our understanding of the biology of obesity requires simultaneous assessment of energy intake and energy expenditure, and of the means to determine their net effects on energy balance repeatedly in individual animals. The Specific Aims of the APC are to provide state-of-the-art assessments of: 1) Adipose Tissue Morphology & Function, in terms of cellular, histologic and functional analyses; 2) Body Composition, in terms of the total relative fat and lean mass, and whole body anatomical distribution of adipose tissue; 3) Energy intake and Expenditure, including oxygen and CO2 consumption, respiratory quotient, and thermogenesis in brown adipose tissue; and 4) consequences of Metabolic Procedures and Surgeries, designed to illuminate the physiology of weight regulation and obesity pathophysiology, including gastrointestinal bypass, autonomic denervations and central nervous system targeting using viral, optogenetic and chemogenetic approaches..

动物表型核心项目概要/摘要肥胖的啮齿动物模型为肥胖研究和所做的工作提供了无价的价值。 NYONRC 调查员。为了满足中心研究人员不断增长的需求，拟议的 NYONRC 动物表型核心 (APC) 反映了动物能量平衡核心 (Einstein) 的显着扩展和与脂肪组织核心（哥伦比亚）的动物成分整合。在过去的4年里在本周期中，NYONRC 的这些组件已处理了 38 个国家/地区的 14,000 多个服务请求。 NYONRC 成员获得 45 项拨款支持，并向 9 名 NIH 资助的非 NYONRC 提供额外服务成员。 APC 提供的服务有助于获得 19 项新的或续签的赠款，并产生了 213 种出版物。拟议的 APC 集中并协调扩大了该合并核心的技术和研究能力。加入额外的动物爱因斯坦和哥伦比亚校区的资源将显着增加用户和研究基础 NYONRC 整体，特别是 APC 核心。通过协调应用 NYONRC 专用哥伦比亚大学和爱因斯坦大学的设施、资源和专业知识，拟议的 APC 核心将结合对摄入行为进行复杂的定性和定量测量，同时评估能量消耗、体力活动、体温和可重复的无创身体成分通过 MRI 和 MicroCT 进行分析。还可以进行一系列代谢手术，包括胃肠道手术搭桥、自主去神经手术和脂肪组织服务，包括：1) 量化脂肪细胞亚群（棕色、白色、米色）的大小、变异和细胞超微结构； 2）原代细胞分离和分析； 3）脂肪细胞和脂肪组织巨噬细胞的分化； 4) 脂肪组织移植。服务范围的扩大源于我们日益认识到肥胖的生物学需要同时评估能量摄入和能量消耗，以及是指在个体动物中反复确定它们对能量平衡的净影响。 APC 的具体目标是提供最先进的评估： 1) 脂肪组织形态学和功能，在细胞、组织学和功能分析方面； 2) 身体成分，在总相对脂肪和瘦肉质量以及脂肪组织的全身解剖分布； 3）能量摄入和消耗，包括氧气和二氧化碳消耗、呼吸商和棕色脂肪组织的产热作用； 4) 代谢程序和手术的后果，旨在阐明体重调节的生理学和肥胖病理生理学，包括使用病毒、光遗传学靶向胃肠道绕道、自主神经支配和中枢神经系统和化学遗传学方法..