Animal Physiology Core

动物生理学核心

基本信息

批准号：
8872949
负责人：
GARY J SCHWARTZ
金额：
$ 12.05万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-10 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8872949
关键词：
Adipose tissue Animals Awareness Behavior Behavioral Brain Carbohydrates Cardiac Circadian Rhythms Complications of Diabetes Mellitus Core Facility Data Denervation Diabetes Mellitus Diet Drug or chemical Tissue Distribution Eating Energy Metabolism Exercise Experimental Animal Model Experimental Models Feeding behaviors Functional Magnetic Resonance Imaging Gastric Bypass Gastrointestinal tract structure Glucose Glycogen Goals Homeostasis Hyperglycemia Image Analysis Indirect Calorimetry Instruction Insulin Laboratories Light Lipids Liver Magnetic Resonance Spectroscopy Measurement Mediating Metabolic Metabolism Methodology Mission Molecular Motor Activity Mus Muscle Mutation NMR Spectroscopy Neuraxis Obesity Operative Surgical Procedures Pancreas Patients Performance Phenotype Physiological Physiology Play Postdoctoral Fellow Procedures Productivity Rattus Recording of previous events Regulation Research Research Methodology Research Personnel Resources Rodent Rodent Model Role Scanning Schedule Scientist Services Signal Transduction Skeletal Muscle Students Surgical Models Systems Biology Techniques Temperature Thermogenesis Tissues Work bariatric surgery base blood glucose regulation cost effective design detection of nutrient diet and exercise energy balance fatty acid metabolism fatty acid oxidation gastrointestinal glucose metabolism glucose tolerance improved in vivo innovation insulin sensitivity intrahepatic metabolic abnormality assessment microCT microPET novel relating to nervous system tool

项目摘要

The Animal Physiology Core (APC) employs sophisticated research methodologies to assist Einstein-Mount Sinai Diabetes Research Center (ES-DRC) investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. Through collaborative efforts with the other Cores of the ES-DRC, the APC enables investigators to thoroughly characterize the effects of defined pharmacological, dietary, environmental and genetic alterations on glucose and lipid homeostasis, insulin action, and metabolism. The Core performs studies of adiposity distribution and facilitates NMR spectroscopy, fMRI and microPET analysis of experimental animal models of diabetes undergoing metabolic studies. The Core also provides specialized rodent surgeries for investigator laboratories and several cardiac functional assessments related to diabetic complications. To accomplish these goals, the Animal Physiology Core will: 1) advise investigators in the design of metabolic studies relevant to the control of glucose homeostasis and insulin action in rodents; 2) make available to investigators specialized measurements of whole body and tissue-specific glucose sensitivity and insulin action including, but not limited to insulin, pancreatic and hyperglycemic clamp studies; 3) provide specialized surgical models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism; 4) offer instruction to students, postdoctoral fellows, investigators and technical staff in performing surgical and physiological techniques necessary to evaluate the controls of glucose homeostasis and insulin action; 5) provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, feeding behavior, and locomotor activity using specialized metabolic (indirect calorimetry) and behavioral rodent cages; 6) provide assessment of the effects of spontaneous or scheduled exercise on glucose homeostasis and metabolism; 7) make available to investigators specialized measurements of rodent adipose tissue distribution using microCT and measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using NMR; 8) make available to investigators specialized measurements of brain energy and glucose utilization by functional MRI (fMRI) and microPET scanning; and 9) coordinate these efforts with other ES-DRC and Institutional Core facilities at Einstein and Mount Sinai. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this Core.

动物生理核心（APC）采用复杂的研究方法来协助爱因斯坦安装西奈糖尿病研究中心（ES-DRC）研究人员在体内评估葡萄糖和脂肪酸评估中小鼠和大鼠的代谢，胰岛素敏感性和能量稳态。通过与 ES-DRC的其他核心，APC使研究人员能够彻底表征定义的影响葡萄糖和脂质稳态的药理，饮食，环境和遗传改变，胰岛素作用和新陈代谢。核心进行肥胖分布的研究并促进NMR光谱，糖尿病实验动物模型的FMRI和MICROPET分析正在进行代谢研究。这 Core还为研究人员实验室和几个心脏功能提供专门的啮齿动物手术与糖尿病并发症有关的评估。为了实现这些目标，动物生理核心将：1）建议研究人员在与葡萄糖稳态控制和控制的代谢研究设计方面的建议啮齿动物的胰岛素作用； 2）向调查人员提供全身的专门测量组织特异性葡萄糖敏感性和胰岛素作用，包括但不限于胰岛素，胰腺和高血糖夹； 3）提供专门的手术模型来研究胰岛素敏感性，能量平衡，葡萄糖和脂肪酸代谢； 4）向学生提供指导，博士后研究员，调查人员和技术人员在进行评估的手术和生理技术中控制葡萄糖稳态和胰岛素作用； 5）提供全身碳水化合物/脂肪酸的分析使用专门代谢（间接代谢量热法）和行为啮齿动物笼； 6）评估自发或计划的影响运动对葡萄糖稳态和代谢； 7）向调查人员提供专门的使用MicroCT和肝脏中糖原的测量来测量啮齿动物脂肪组织分布使用NMR肌肉，肝内脂质和细胞内脂质； 8）向调查人员提供专门的通过功能MRI（fMRI）和MicroPET扫描来测量脑能量和葡萄糖利用；和9）与爱因斯坦和西奈山的其他ES-DRC和机构核心设施进行协调。全部这些服务适用于新的糖尿病研究的调查人员，以及从事的调查人员通过使用专业知识和设施可以丰富和扩展糖尿病相关的项目核。