Biological Evaluation Shared Resource (BE-SR)

生物学评价共享资源 (BE-SR)

• 批准号: 9369063
• 负责人: BENNETT David ELZEY
• 金额: $ 12.52万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9369063
• 关键词: Antineoplastic Agents; Biological; Biology; Cancer Biology; Cancer Center Support Grant; Cancer Model; Cell Line; Consultations; Data; Development; Diagnosis; Evaluation; Experimental Designs; Foundations; Funding; Goals; Grant; Human; Immunocompromised Host; In Vitro; Malignant - descriptor; Malignant Neoplasms; Measures; Mission; Modeling; Molecular; Mus; NCI Center for Cancer Research; Neoplasm Metastasis; New Agents; Nude Mice; Pathway interactions; Peer Review; Play; Preparation; Procedures; Protocols documentation; Quality Control; Reproducibility of Results; Research; Research Personnel; Resource Sharing; Role; Services; Standardization; Technology; Testing; Toxicity Tests; Transgenic Animals; Transplantation; Tumor Cell Invasion; Universities; War; Xenograft procedure; animal model development; base; cost; drug development; efficacy study; in vivo; member; mouse model; response; tool; tumorigenesis

Biological Evaluation Shared Resource: Project Summary Biology Shared Resource Group In cancer biology, mouse models continue to play significant roles in studies of tumor invasion, metastasis and malignant transformation, as well as in studies examining responses to therapy. Key advancements have emerged in the development of animal models for cancer biology, including the advent of orthotopic models for metastasis, transgenic animals that have developmental pathways to tumorigenesis, and state-of-the-art immunocompromised strains. Transplanted human xenografts remain a primary tool for molecular discovery and evaluation. Despite their flaws and shortcomings, xenograft mouse models have played a significant role in cancer drug development in the past three decades, and mouse models will continue to be a foundation in the war against cancer. At the Purdue University Center for Cancer Research (PCCR) where a vast pipeline of potential new agents for diagnosing and treating cancer are emerging, researchers need a productive and established facility for in vivo testing which can navigate the arena of murine cancer models. The mission of the Biological Evaluation Shared Resource (BE-SR) is to provide expert guidance to investigators in grant preparation, model selection and experimental design, and to perform toxicity testing and proof-of-concept efficacy studies to advance their projects using in vivo testing. In addition to including cell line-based xenograft testing in nude mice, the BE-SR, in its efforts to offer the latest technologies, is transitioning to the use of NSG mice and towards the use of primary xenograft testing. The goal of the BE-SR is to provide high quality, reproducible results that are a consequence of standardized procedures and protocols and of carefully maintained implantable cell lines that are subjected to strict quality control measures both in vitro and in vivo. This quality service will result in overall lower costs and higher data fidelity to the investigator through smaller standard deviations, fewer experimental repeats to achieve statistical significance, and higher biologic relevance through initial consultation and guidance.

生物评估共享资源：项目摘要 生物学共享资源组 在癌症生物学中，小鼠模型继续在肿瘤侵袭，转移研究中起重要作用 和恶性转化，以及研究对治疗反应的研究。关键进步已有 出现在癌症生物学动物模型的开发中，包括原位模型的出现 转移，具有肿瘤发生途径的转基因动物，最新的 免疫功能低下的菌株。移植的人异种移植仍然是分子发现的主要工具 和评估。尽管存在缺陷和缺点，但异种移植鼠标模型还是发挥了重要作用 在过去的三十年中，在癌症药物开发中，鼠标模型将继续成为 反对癌症的战争。普渡大学癌症研究中心（PCCR），那里有大量的管道 潜在的诊断和治疗癌症的新药物正在出现，研究人员需要生产力， 建立的体内测试设施，可以导航鼠类癌模型的舞台。任务 生物评估共享资源（BE-SR）是为Grant的研究人员提供专家指导 准备，模型选择和实验设计，并进行毒性测试和概念证明 效力研究可以使用体内测试来推进其项目。除了包括基于细胞系的异种移植 在裸鼠中进行测试，BE-SR在提供最新技术的努力中正在过渡到NSG的使用 小鼠和使用主要异种移植测试。 BE-SR的目标是提供高质量 可再现的结果是标准化程序和协议的结果以及仔细的 维护的可植入细胞系受到严格的体外和体内质量控制测量。 这项高质量的服务将通过较小 标准偏差，实验重复较少以获得统计显着性，并且更高的生物学 通过初步咨询和指导的相关性。