BSLII Stationed Zeiss 880 Confocal Microscope with Airyscan

BSLII 固定式蔡司 880 共焦显微镜，带 Airyscan

• 批准号: 9274504
• 负责人: Havva Fitnat Yildiz
• 金额: $ 45.56万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274504
• 关键词: Animal Organ; Biological Sciences; Biomedical Research; California; Cell Communication; Cell Cycle; Confocal Microscopy; Core Facility; Equipment; Eye diseases; Funding; Immune; Immune Tolerance; Investigation; Lasers; Microbial Biofilms; Microscope; Microscopy; Movement; National Eye Institute; National Institute of Allergy and Infectious Disease; National Institute of General Medical Sciences; Pathogenesis; Request for Proposals; Research; Research Personnel; Retinal; Risk; Sampling; Scanning; Science; System; Training; United States National Institutes of Health; Universities; Work; cost effective; flexibility; fluorescence imaging; graduate student; instrument; next generation; programs

This proposal requests funds to purchase a laser scanning confocal microscope for a group of NIH-funded investigators from several departments at the University of California at Santa Cruz. The proposed Zeiss 880 system with Airyscan will replace our current Zeiss PASCAL confocal microscope, which was purchased through NIH's SIG mechanism and installed in 2004. The Zeiss LSM 880 is a flexible and modular confocal laser scanning microscope for high end fluorescence imaging. The LSM 880 is an outstanding choice for a core facility as it is well suited for the research objectives of our users, it is easy to use and provides a cost- effective solution to our research challenges. The proposed instrument is essential for NIH-funded biomedical research taking place at UC Santa Cruz. The major user group is funded by multiple NIH programs. These are the National Institute of Allergy and Infectious Diseases (NIAID), The National Institute of General Medical Sciences (NIGMS), National Eye Institute (NEI). They seek to bring the power of confocal microscopy to studies of bacterial pathogenesis and mechanism of biofilm formation, bacterial movement within animal organs, and immune cell interactions during immunological tolerance, investigation of cell cycle, degenerative eye diseases and retinal plasticity. This microscope system will become an integral part of UCSC's Life Sciences Microscopy Center, and will located in a BSLII designated space within our Biomedical Sciences building. This placement is required for work on Risk Group 2 samples. The facility is open to all NIH-funded investigators; thus, this equipment will enhance and sustain biomedical research on this campus, while providing advanced training to our graduate students and postdoctoral research fellows, thereby helping to create the next generation of biomedical investigators.

该建议要求资金购买一组NIH资助的激光扫描共聚焦显微镜 来自加利福尼亚大学圣克鲁斯分校的多个系的调查人员。拟议的蔡司880 使用Airyscan的系统将取代我们当前的Zeiss Pascal共聚焦显微镜，该显微镜被购买 通过NIH的SIG机制，并于2004年安装。Zeiss LSM 880是一种灵活而模块化 用于高端荧光成像的激光扫描显微镜。 LSM 880是A 核心设施非常适合我们用户的研究目标，它易于使用，并提供成本 - 有效解决我们的研究挑战。提出的仪器对于NIH资助的生物医学至关重要 在UC Santa Cruz进行的研究。主要用户组由多个NIH计划资助。这些都是 美国国家通用医学研究所（NIAID）国家过敏和传染病研究所（NIAID） 国家眼科研究所（NEI）科学（NIGMS）。他们试图将共聚焦显微镜的力量带到 生物膜形成的细菌发病机理和动物中细菌运动的机制的研究 器官和免疫耐受性期间的免疫细胞相互作用，研究细胞周期，退化性 眼病和视网膜可塑性。该显微镜系统将成为UCSC生活不可或缺的一部分 科学显微镜中心，将位于我们生物医学的BSLII指定空间中 建筑。在风险第2组样品上工作需要此放置。该设施向所有NIH资助开放 调查人员；因此，该设备将在该校园内增强和维持生物医学研究，而 向我们的研究生和博士后研究研究员提供高级培训，从而帮助 创建下一代生物医学研究人员。

项目成果

A Conserved PP2A Regulatory Subunit Enforces Proportional Relationships Between Cell Size and Growth Rate.

保守的 PP2A 调节亚基强制细胞大小和生长速率之间的比例关系。

• DOI: 10.1534/genetics.119.301012
• 发表时间: 2019
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Leitao,RicardoM;Jasani,Akshi;Talavara,RafaelA;Pham,Annie;Okobi,QuincyJ;Kellogg,DouglasR
• 通讯作者: Kellogg,DouglasR