Immune Responses To Ocular Antigens

对眼抗原的免疫反应

• 批准号: 9555659
• 负责人: Igal Gery
• 金额: $ 18.38万
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9555659
• 关键词: Address; Affect; Allergic Conjunctivitis; Annual Reports; Antigens; Area; Blindness; CD4 Positive T Lymphocytes; Cell Count; Cells; Data; Development; European; Eye; Eye diseases; Flow Cytometry; Goblet Cells; Hyperplasia; Hypersensitivity; Immune; Immune response; Immunology; Immunosuppressive Agents; Inflammation; Inflammatory; Interferon Type II; Interleukin-17; Journals; Lymphocyte Subset; Manuscripts; Measures; Mediating; Methods; Mus; New Agents; Pathogenicity; Pattern; Process; Published Comment; Reaction; Research; Spleen; Staining method; Stains; Testing; Th2 Cells; Tissues; autoimmune uveitis; conjunctiva; eosinophil; inhibitor/antagonist; interest; migration; novel strategies; transcription factor; treatment group

NEW INFORMATION ON THE DIFFERENCES BETWEEN Th1/Th17 AND Th2/Th9 CELLS IN THEIR MIGRATION INTO EYE TISSUES Data collected in this study (initiated and detailed in previous Annual Reports, mostly in FY 2015) were summarized in a manuscript submitted to Experimental Eye Research. Reviewers comments requested additional information on two issues: (i) The effect of the conjunctival inflammation in recipients of Th2/Th9 cells on goblet cells in this tissue. These cells were identified by staining with PAS. Unlike the typical increase in goblet cell number (hyperplasia) in classical allergic conjunctivitis, the number of goblet cells did not change in conjunctivae of mice with allergy-like reaction induced by Th2 cells. (ii) Measuring the proportion of eosinophils among the inflammatory cells in the recipient conjunctivae revealed the presence of eosinophils throughout the affected tissue, reaching up to 50% in areas in which eosinophils were aggregated. NEW INFORMATION CONCERNING THE IMMUNOMODULATING AGENT TMP778 Data collected in this study, detailed in previous Annual Reports, were summarized and submitted in a manuscript to the European Journal of Immunology. To address reviewers comments, we analyzed the eye infiltrating cells in mice treated with TMP778 or the vehicle control. The method we employed was intracellular flow cytometry and the collected data revealed that (i) the proportions of positive cells among total CD4 cells was approximately twice larger than the proportions found among CD4 cells in the spleen of the tested mice; (ii) similar to observations in the spleen, the proportion of infiltrating cells expressing interferon-gamma (IFN-g) was larger than of cells expressing interleukin (IL)-17 (This finding is of interest, since TMP778 is a selective inhibitor of RORgt, the transcription factor for IL-17.

有关Th1/Th17和Th2/Th9细胞在迁移到眼组织中的差异的新信息 在本研究中收集的数据（在先前的年度报告中启动并详细介绍，主要是在2015财年）进行了总结，该手稿已提交给实验性眼睛研究。审稿人评论要求有关两个问题的其他信息： （i）结膜炎症对Th2/Th9细胞受体对该组织中的杯状细胞的影响。通过用PAS染色来鉴定这些细胞。与经典过敏性结膜炎中的杯状细胞数量（增生）的典型增加不同，杯状细胞的数量没有改变小鼠的结膜变化，而Th2细胞引起的过敏反应。 （ii）在受体结膜中测量炎症细胞中嗜酸性粒细胞的比例表明，在整个受影响的组织中存在嗜酸性粒细胞，在聚集嗜酸性粒细胞的区域中最高可达到50％。 有关免疫调节剂TMP778的新信息 在以前的年度报告中详细介绍的本研究中收集的数据总结了欧洲免疫学杂志的手稿。为了解决审阅者的评论，我们分析了用TMP778或车辆控制治疗的小鼠中的眼睛浸润细胞。我们采用的方法是细胞内流式细胞术，收集的数据表明，（i）总CD4细胞中阳性细胞的比例大约是测试小鼠脾脏中CD4细胞中发现的比例的两倍； （ii）类似于脾脏中的观察结果，表达干扰素γ（IFN-G）的浸润细胞的比例大于表达白介素（IL）-17的细胞（由于TMP778是RORGT的选择性抑制剂，IL-17的转录因子是IL-17的转录因子。

项目成果

The unique features of Th9 cells and their products.

• DOI: 10.1615/critrevimmunol.v32.i1.10
• 发表时间: 2012
• 期刊: Critical reviews in immunology
• 影响因子: 1.3
• 作者: Tan C;Gery I
• 通讯作者: Gery I

Induced regulatory T-cells (iTregs) generated by activation with anti-CD3/CD28 antibodies differ from those generated by the physiological-like activation with antigen/APC.

• DOI: 10.1016/j.cellimm.2014.06.004
• 发表时间: 2014-08
• 期刊: Cellular immunology
• 影响因子: 4.3
• 作者: Chan Zhao;G. Shi;B. Vistica;Samuel J. H. Hinshaw;Wambui S. Wandu;Cuiyan Tan;Meifen Zhang;I. Gery