Enabling Nanomembrane-Based Biomolecule and Nanoparticle Separations

实现基于纳米膜的生物分子和纳米颗粒分离

基本信息

批准号：
9045849
负责人：
James L McGrath
金额：
$ 19.89万
依托单位：
SIMPORE, INC.
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-19 至 2018-01-18
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9045849
关键词：
Academia Antibodies Area Biological Sciences Biotechnology Buffers Caliber Centrifugation Complex Custom Data Development Devices Diagnostics Research Dialysis procedure Drug Delivery Systems Goals Housing Immunoglobulin G Laboratories Letters Manufacturer Name Marketing Membrane Methods Molecular Molecular Sieve Chromatography Pain Particle Size Performance Permeability Phase Polymers Polystyrenes Preparation Proteins Quantum Dots Reporting Resort Sampling Scheme Scientist Silicon Small Business Technology Transfer Research Specificity Speed Surface Techniques Technology Testing Time Tissues Tube Universities antibody conjugate base biomedical scientist design drug development experience in vivo imaging innovation meetings nanomembrane nanoparticle particle product development professor prototype public health relevance solute tool

项目摘要

DESCRIPTION (provided by applicant): The global market for nanoparticles (NP) in biotechnology, drug development and drug delivery was estimated to be $20B for 2013 and is expected to reach more than $50B by 2017. The two most active areas of product development are NPs for drug delivery and for in vivo imaging. In these applications it is often critical that he outer surfaces of NPs are functionalized with antibodies (Ab) that target them to speciﬁc tissues After functionalizing NPs with Abs, it is necessary to remove unbound Ab from the NP preparation because any free Ab will compete with the NPs for target tissues. Given the similar sizes of Ab (~ 14 nm) and NPs, this ubiquitous clean-up step is challenging and represents a current pain in the market place. Both academic and industrial NP scientists report that existing polymer- based membranes are not capable of performing this separation and that they must resort to more time-consuming, complex, and lower yield techniques such as size-exclusion chromatography or dialysis. Membrane-based methods are preferred because of their simplicity, efﬁciency and scalability. In this proposal, we will realize a near-term, straight-forward produc development opportunity that will result in a solution to the NP-Ab separation problem. Using SiMPore's nanomembranes in a custom centrifuge conﬁguration, we have recently shown the ability to separate Ab from 20 nm polystyrene particles. Additional innovations in membrane fabrication and non-fouling coatings will allow us to pursue the following goals: 1) to develop and evaluate a SiMPore product prototype based on our preliminary custom centrifuge concept, 2) to produce a sufﬁcient number of prototypes to enable beta- testing at two major life science tool suppliers, and 3) to introduce a validated product upon completion of this 9-month Phase I project.

描述：生物技术，药物开发和药物输送的全球纳米颗粒市场（NP）的2013年为20B美元，到2017年被曝光将超过$ 50B。产品开发的两个最活跃的领域是交付的NPS对于体内成像。与NP竞争目标组织。诸如尺寸排斥色谱或透析之类的技术是因为它们在此提案中的效率和可伸缩性构造，将AB与20 nm聚苯乙烯颗粒分开的能力。该9个月I阶段项目完成后的产品。