Neural Processing in Behaving Adolescent Rats

青春期大鼠行为的神经处理

基本信息

批准号：
8454981
负责人：
Nicholas W Simon
金额：
$ 5.39万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8454981
关键词：
Address Adolescence Adolescent Adult Affect Age Animal Model Anterior Attention Basic Science Behavior Behavioral Behavioral inhibition Biological Neural Networks Brain Brain region Climacteric Cognitive Communication Complex Corpus striatum structure Cues Data Data Analyses Development Dissociation Dorsal Drug Addiction Drug abuse Electrophysiology (science)Ensure Event Goals Intervention Investigation Measures Mediating Mental disorders Methodology Nature Neurobiology Neurons Noise Outcome Pattern Performance Phase Prefrontal Cortex Procedures Process Public Health Rattus Rodent Staging Structure Testing Time Training addiction age group age related base cingulate cortex design expectation experience information processing insight motivated behavior novel public health relevance relating to nervous system research study response social stressor

项目摘要

DESCRIPTION (provided by applicant): This application outlines a training plan and experimental procedures to ensure that the applicant will receive technical and intellectual development toward independence. The application focuses on neuronal bases of motivated behavior in adolescents, a topic with important implications for basic science and public health. Adolescents are particularly vulnerable to drug addiction and other psychopathological illnesses. This vulnerability may be a result of ongoing prefrontal cortex (PFC) and striatal development during adolescence. However, little is known about how neuronal activity within these brain regions during the expression of motivated behavior differs as a function of age. The applicant has developed the Cued Response Inhibition Task, or CRIT, to measure the ability to withhold a response during an inhibitory cue, then respond promptly following cue termination. Preliminary data demonstrate that adolescent rats are unable to respond as quickly as adults following cue termination, a behavioral difference that appears to be related to inability to adequately sustain attention to the cue. It is hypothesized that this behavioral profile will be associated with aberrant neuronal encoding of task events in PFC and striatal subregions in adolescent compared to adult rats. To test this hypothesis, two aims are proposed, both of which involve application of methodologies and data analysis that are novel for the applicant. The first aim will involve recording single unit activity simultaneously from two brain regions during CRIT performance: anterior cingulate cortex (ACC) and dorsal striatum (DS), These regions were chosen because previous rodent recording studies demonstrate that ACC phasic responses correlate with sustained attention, and that neuronal encoding in the DS differs in adolescents from adults during motivated behavior. Neural activity will be analyzed during cue presentation, instrumental response, and outcome expectation/delivery, then compared between adults and adolescents. In the second aim, neuronal interactions within ACC and DS, as well as communication between ACC and DS during CRIT will be quantified. This will involve within, and between region analysis of noise correlations between unit pairs, local field potential (LFP) spectral power, and phase-locking between single unit activity and LFP. This will provide information about communication and direction of information flow between ACC and DS, and how this relates to behavior in adolescent and adult rats. Collectively, these experiments will provide novel information on how neural processing differs between adult and adolescent rats, and how these differences correlate with age related behavioral differences.

描述（由申请人提供）：本申请概述了培训计划和实验程序，以确保申请人获得独立的技术和智力发展。该应用程序侧重于青少年动机行为的神经元基础，这是一个对基础科学和公共卫生具有重要影响的主题。青少年特别容易吸毒成瘾和其他精神疾病。这种脆弱性可能是青春期期间前额皮质（PFC）和纹状体持续发育的结果。然而，人们对这些大脑区域内的神经元活动在动机行为表达过程中如何随年龄而变化的情况知之甚少。申请人开发了提示响应抑制任务（CRIT），以衡量在抑制提示期间抑制响应，然后在提示终止后迅速响应的能力。初步数据表明，青春期大鼠在提示终止后无法像成年大鼠那样快速做出反应，这种行为差异似乎与无法充分维持对提示的注意力有关。据推测，与成年大鼠相比，这种行为特征与青少年前额皮层和纹状体亚区任务事件的神经元编码异常有关。为了检验这一假设，提出了两个目标，这两个目标都涉及对申请人来说新颖的方法和数据分析的应用。第一个目标将涉及在 CRIT 表现期间同时记录两个大脑区域的单个单位活动：前扣带皮层 (ACC) 和背侧纹状体 (DS)，选择这些区域是因为之前的啮齿动物记录研究表明 ACC 阶段性反应与持续注意力相关，并且神经元编码在动机行为过程中，青少年的 DS 与成人不同。将在提示呈现、仪器反应和结果预期/交付期间分析神经活动，然后在成人和青少年之间进行比较。在第二个目标中，ACC 和 DS 内的神经元相互作用以及 CRIT 期间 ACC 和 DS 之间的通信将被量化。这将涉及单元对之间的噪声相关性的区域内和区域间分析、局部场电位 (LFP) 频谱功率以及单个单元活动和 LFP 之间的锁相。这将提供有关 ACC 和 DS 之间的通信和信息流方向的信息，以及这与青少年和成年大鼠的行为有何关系。总的来说，这些实验将提供关于成年和青少年大鼠的神经处理有何不同，以及这些差异如何与年龄相关的行为差异相关的新信息。