Biochemical-Analysis Core

生化分析核心

• 批准号: 8381143
• 负责人: MYRON GOODMAN
• 金额: $ 18.14万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8381143
• 关键词: 2-Aminopurine; Anisotropy; Biochemical; Biological Assay; Chemicals; Complex; DNA-Directed DNA Polymerase; Data; Data Analyses; Dinucleotide Repeats; Drug Formulations; Enzyme Kinetics; Fluorescence; High Pressure Liquid Chromatography; Kinetics; Label; Left; Measurement; Modification; Nucleosides; Nucleotides; Oxygen; Preparation; Radio; Reaction; Reporter; Services; Students; Techniques; Time; Training; analog; bisphosphonate; chemical synthesis; data acquisition; design; enzyme mechanism; fluorophore; innovation; inorganic phosphate; mutant; nucleotide analog; protein purification; research study; stopped-flow fluorescence; theories

Core B is a biochemical synthesis and analysis core that will provide indispensable services for all three projects. The synthetic component of Core B is designed to perform large scale synthesis, purification and analysis of nucleotide analogs for use in Projects 1 and 3. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DMA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in chemical synthesis and protein purification techniques and in the principles and practice of enzyme kinetic analysis.

Core B是生化合成和分析核心，将为这三者提供不可或缺的服务 项目。 Core B 的合成成分旨在进行大规模合成、纯化和 项目1和3中使用的核苷酸类似物的分析。核心B的生化成分被设计 进行蛋白质纯化并合成项目 1 和 3 所需的专门 DMA 构建体。 分析组件将负责获取动力学数据并对其进行全局分析， 包括前稳态停流荧光强度和旋转各向异性和快速猝灭 实验，用于支持项目 2 和 3。核心 B 将作为培训的主要工具 化学合成和蛋白质纯化技术以及化学合成和蛋白质纯化技术领域的研究生和博士后学生 酶动力学分析原理与实践。