CORE--Biochemical-Analysis Core

CORE--生化分析核心

• 批准号: 7479346
• 负责人: MYRON GOODMAN
• 金额: $ 24.01万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7479346
• 关键词: 2-Aminopurine; Addendum; Anisotropy; Biochemical; Computer Analysis; DNA; DNA-Directed DNA Polymerase; Data; Enzyme Kinetics; Facility Construction Funding Category; Fluorescence; Gel; Hand; Homologous Gene; Human; Kinetics; Label; PCNA gene; Polymerase; Preparation; Property; Protein Overexpression; Protocols documentation; Radioactive; Reagent; Services; Slide; Structural Genes; Students; Supervision; Techniques; Time; Training; Trinucleotide Repeat Expansion; base; data acquisition; design; enzyme mechanism; gel electrophoresis; mutant; pol genes; protein purification; research study; stopped-flow fluorescence; vector

Core B is a biochemical analysis core that will provide indispensable services for all three projects. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DNA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in protein purification techniques and in the principles and practice of enzyme kinetic analysis.

Core B是生化分析核心，将为这三个项目提供不可或缺的服务。 Core B 的生化组件旨在进行蛋白质纯化并合成项目 1 和 3 所需的专门 DNA 构建体。分析组件将负责获取和执行动力学数据的全局分析，包括稳态停流荧光强度以及旋转各向异性和快速淬灭实验，用于支持项目 2 和 3。Core B 将作为培训研究生和博士后学生蛋白质纯化技术以及酶动力学分析原理和实践的主要工具。