Non-heme Iron Nitrosyl Complexes: Structures, NO Release & Applications

非血红素亚硝酰铁络合物：结构，NO 释放

基本信息

批准号：
9304240
负责人：
LIJUAN LI
金额：
$ 11.06万
依托单位：
CALIFORNIA STATE UNIVERSITY LONG BEACH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2019-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9304240
关键词：
Biological Biological Process Biological Testing Blood Pressure California Cardiac Cardiovascular system Cell Culture System Cell Culture Techniques Cell physiology Characteristics Chelating Agents Chemicals Collaborations Communities Complex Cytotoxic agent DNA Data Drug Kinetics Electrochemistry Environment Evolution Family Goals Human Imidazole Immune system In Situ In Vitro Investigation Kinetics Knowledge Lasers Ligands Measures Medicine Memory Metals Methods Mono-S Muscle relaxation phase Neurosciences Nitric Oxide Pharmaceutical Preparations Pharmacology Study Phenanthrolines Phosphines Physical Chemical Technique Physiology Platelet aggregation Play Property Reaction Research Role Series Smooth Muscle Sodium Sodium Chloride Structure Sulfhydryl Compounds Sulfonic Acids Techniques Test Result Testing Transition Elements Universities Washington Water X-Ray Crystallography base biological systems biosynthetic product cancer therapy in vivo insight interest iron nitrosyl long term memory metal complex nanosecond photolysis prevent public health relevance triphenylphosphine tris(2-carboxyethyl)phosphine

项目摘要

DESCRIPTION (provided by applicant): Recent discoveries in the roles of nitric oxide in humans have generated tremendous interest in biologically active transition metal nitrosyl complexes. Among them, the non-heme iron nitrosyl complexes have been identified as products of biosynthetic evolution of nitric oxide, suggested as nitric oxide storage, and found to be responsible for several important biological functions. However, these studies were limited to EPR and IR identification of these complexes in solution and the structures of these compounds were not well characterized and understood. The proposed research is to synthesize non-heme iron nitrosyl compounds that mimic biologically active non-heme iron nitrosyls by using specially selected ligands that are soluble in water and have the potential to interact with DNA. The reaction mechanisms and structures of these complexes will be studied using a combination of spectroscopic methods, such as FT-IR, EPR, NMR, UV-vis, X-ray crystallography and electrochemical techniques. Chemical, photolytic and electrochemical methods will be used to achieve the NO release from these complexes and the quantitative NO release kinetics will be investigated by applying nanosecond laser photolysis techniques. Potential biological activities will also be tested. These results will provide information on the structures of biologically activ non-heme iron nitrosyls, thus helping the neuroscience, physiology and medicinal research communities understand the diverse biological functions of nitric oxide and its metal complexes, some of which include controlling blood pressure, preventing platelet aggregation, acting as biological messengers and immune system cytotoxic agents, and playing a role in long-term memory. The kinetic data and the biological activity testing results will be used to identify the NO-releasing metal complexes, which can be used in cardiovascular muscle relaxation, cancer therapy, pharmacokinetic studies, and other medicinal applications.

描述（由申请人提供）：一氧化氮在人类中的作用的最新发现对生物活性过渡金属亚硝基复合物产生了极大的兴趣。其中，非血红素亚硝基复合物已被确定为一氧化氮的生物合成进化产物，建议是一氧化氮储存，发现为负责几种重要的生物学功能。但是，这些研究仅限于EPR，并且在溶液中对这些复合物的IR鉴定，这些化合物的结构没有很好地表征和理解。拟议的研究是通过使用特殊选择的配体可溶于水中并具有与DNA相互作用的潜力，从而合成非血红素亚硝基化合物，以模拟生物活性非血红素亚硝基基。这些复合物的反应机理和结构将使用光谱方法组合进行研究，例如FT-IR，EPR，NMR，UV-VIS，X射线晶体学和电化学技术。化学，光解和电化学方法将用于从这些复合物中释放NO，并且定量NO释放动力学将通过应用纳秒激光光解技术研究。潜在的生物活动也将进行测试。这些结果将提供有关生物活性非血红素亚硝基铁结构的信息，从而有助于神经科学，生理学和药物研究社区了解一氧化氮及其金属复合物的多样化生物学功能，其中一些包括控制血压，可预防血小板聚集，可预防血小板聚集，作为生物学启动和免疫系统的氧化剂和免疫毒素的作用，并扮演长期的角色，并扮演了良好的角色。动力学数据和生物活性测试结果将用于鉴定不释放的金属复合物，该复合物可用于心血管肌肉松弛，癌症治疗，药代动力学研究和其他药用应用。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Recent Advances in Multinuclear Metal Nitrosyl Complexes.

DOI：
10.1016/j.ccr.2015.03.026
发表时间：
2016-01-01
期刊：
Coordination chemistry reviews
影响因子：
20.6
作者：
Li L;Li L
通讯作者：
Li L

The Preparation, Structural Characteristics, and Physical Chemical Properties of Metal-Nitrosyl Complexes.

DOI：
10.1007/430_2013_101
发表时间：
2013
期刊：
Structure and bonding
影响因子：
0
作者：
Holloway LR;Li L
通讯作者：
Li L

A combined experimental and theoretical study of dinitrosyl iron complexes containing chelating bis(diphenyl)phosphinoX (X = benzene, propane and ethylene): X-ray crystal structures and properties influenced by the presence or absence of π-bonds in chelat

含有螯合双（二苯基）膦 X（X = 苯、丙烷和乙烯）的二亚硝基铁配合物的综合实验和理论研究：X 射线晶体结构和性质受螯合剂中存在或不存在 α 键的影响

DOI：
10.1016/j.poly.2013.12.019
发表时间：
2014
期刊：
Polyhedron
影响因子：
2.6
作者：
Holloway,LaurenR;Clough,AndrewJ;Li,JessicaY;Tao,EmilyL;Tao,Fu-Ming;Li,Lijuan
通讯作者：
Li,Lijuan