Synthetic Antigen Binder Core

合成抗原结合剂核心

• 批准号: 9351543
• 负责人: ANTHONY A KOSSIAKOFF
• 金额: $ 17.85万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-10 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9351543
• 关键词: Address; Affinity; Characteristics; Collaborations; Communities; Community Outreach; Complex; Crystallization; Crystallography; Custom; Detergents; Engineering; Environment; Epitopes; Fruit; Generations; Goals; Lipids; Location; Membrane Proteins; Methodology; Modification; Molecular Chaperones; Molecular Conformation; Outcome; Performance; Play; Population; Property; Protein Conformation; Proteins; Reagent; Research; Research Infrastructure; Services; Shapes; Site; Specificity; Structural Protein; Structure; Synthetic Antigens; System; Technology; Untranslated RNA; Work; channel blockers; complex IV; design; improved; inhibitor/antagonist; member; nanodisk; non-Native; novel; protein complex; protein function; scaffold; synthetic antibodies; tool

The overarching goal of the Synthetic Antibody Binder Sab Core is to develop a broad range of powerful approaches and provide novel reagents to support the consortium¹s and community¹s research objectives. The Sab Core is uniquely capable to generate in a high-throughput way synthetic antibodies (Sabs) to an extensive range of targets including soluble proteins, protein complexes and membrane proteins. Thus, the infrastructure in place with extensive capability to efficiently generate high-quality affinity reagents that will dramatically accelerate membrane protein research performed within the MPSDC Consortium, as well as in the entire membrane protein research community. The goals of the Sab Core are (i) to provide high-quality synthetic affinity reagents for membrane protein targets using state-of-the-art technologies, (ii) to accelerate structure determination by Sab-assisted crystallography and Cryo-EM and (iii) to develop novel applications of Sabs that will enable Core users to significantly elevate the level of mechanistic understanding of membrane protein functions.

合成抗体粘合剂SAB核心的总体目标是开发广泛 强大的方法范围并提供新型试剂以支持财团 和社区的研究目标。 SAB核心具有独特的能力 高通量方式合成抗体（SABS）与广泛靶标 包括固体蛋白质，蛋白质复合物和膜蛋白。那， 基础设施具有广泛的能力，可以有效产生高质量的亲和力 在内部进行的膜蛋白研究将大大加速的试剂 MPSDC财团以及整个膜蛋白研究社区。 SAB核心的目标是（i）提供高质量的合成亲和力试剂 膜蛋白靶标使用最先进的技术，（ii）加速结构 通过SAB辅助晶体学和冷冻EM和（III）确定新颖 SABS的应用将使核心用户能够显着提高 膜蛋白功能的机械理解。