Bacteriophage to prevent infection by antibiotic-resistant enterococci

噬菌体预防抗生素耐药肠球菌感染

• 批准号: 9018963
• 负责人: CHRISTOPHER J KRISTICH
• 金额: $ 23.01万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2017-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9018963
• 关键词: Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Bacteria; Bacterial Infections; Bacteriophages; Clinical; Collection; Communities; Development; Drug resistance; Ensure; Enterococcus; Gastrointestinal tract structure; Genes; Human; Incidence; Infection; Infection prevention; Intestines; Lytic; Microbe; Modeling; Monitor; Mus; Nosocomial Infections; Phage Receptors; Phase; Population; Population Dynamics; Population Heterogeneity; Property; Research; Resistance; Scourge; Site; Specificity; Systemic infection; Toxic effect; Toxin; Vancomycin; Vancomycin Resistance; Virus; cellular targeting; design; genome sequencing; high reward; high risk; in vivo; innovation; killings; member; microbiome; mouse model; mutant; novel therapeutic intervention; prevent; public health relevance; receptor; resistance gene; resistance mechanism; resistant strain

 DESCRIPTION (provided by applicant): New strategies and therapeutics to overcome the scourge of antibiotic-resistant bacterial infections are urgently needed. Enterococci are among the 3 most common causes of hospital-acquired infections, many of which are caused by enterococcal strains that are resistant to multiple antibiotics, including vancomycin. Systemic infections with antibiotic-resistant enterococci occur subsequent to colonization of the gastrointestinal tract, enabled by antibiotic therapy that leads to massive increases in abundance of drug-resistant enterococci. Thus, strategies to reduce or eliminate GI colonization by antibiotic-resistant enterococci before, during, or after antibiotic therapy represent innovativ approaches to reducing the incidence of antibiotic-resistant enterococcal infection and the spread of antibiotic-resistant isolates. We will use a new mouse model of GI colonization by enterococci with features that mimic the antibiotic-induced enterococcal expansion observed in humans to explore strategies to reduce enterococcal GI colonization. In particular, the research proposed here seeks to use lytic enterococcal bacteriophage to achieve reductions in enterococcal abundance in the mammalian GI tract before or during antibiotic therapy as a means of preventing infection by antibiotic-resistant enterococci.

 描述（由适用提供）：迫切需要克服抗生素抗生素感染的新策略和治疗。肠球菌是医院获得感染的三个最常见原因之一，其中许多是由抗多种抗生素（包括万古霉素）抗性的肠球菌菌株引起的。抗生素肠球菌的全身感染发生在胃肠道的定殖后，这是通过抗生素治疗促成的，从而导致大量耐药肠球菌的丰富性大量增加。这是在抗生素治疗之前，期间或之后通过抗生素抗生素肠球菌减少或消除GI定殖的策略，这代表了降低抗抗生素肠球菌感染的发生率和抗生素抗药性分离株的发病率的创新方法。我们将使用肠球菌的新小鼠GI定植模型，其特征模仿了在人类中观察到的抗生素诱导的肠球膨胀，以探索减少肠球菌GI殖民化的策略。特别是，此处提出的研究试图使用裂解的肠细菌在抗生素治疗之前或期间在哺乳动物胃肠道中降低肠球抽象的降低，以防止抗生素抗抗生素 - 抗生素肠球菌感染。