In vivo patterns of receptor activation by odorants

气味剂激活受体的体内模式

基本信息

批准号：
9029178
负责人：
Timothy S McClintock
金额：
$ 42.68万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-12-01 至 2020-11-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant) This project uses a new in vivo assay that allows the identification of the odorant receptors activated in live mice by any odor, making it possible to identify in one experiment many receptors activated by the odor. This accelerates the identification of receptors sensitive to any odor and when combined with in vitro assays for the function of individual odorant receptors, allows testing of hypotheses about the sets of receptors activated by odorants and odor mixtures. The first aim targets a specific region of the broad universe of odorants, testing whether the overlap in sets of receptors responsive to these odorants depends on their structural similarity. The second aim tests hypotheses about the possible interactions between odorants at odorant receptors. It begins with pairs of odorants that are known or suspected of interacting, mostly by the ability of one odorant to block activation of one or more receptors by another odorant. It then moves on to testing more complex mixtures, seeking to identify interactive effects between odorants at receptors - effects that are fundamental to the characteristic sensation of the odor and the discrimination of one odor from another. In addition to improving our understanding of the mechanisms of odor perception, these experiments provide knowledge expected to facilitate the development of more sophisticated ways of manipulating odor environments to benefit the quality of human life.

描述（由申请人提供）该项目使用了一种新的体内测定法，可以识别活体小鼠中被任何气味激活的气味受体，从而可以在一个实验中识别出许多被气味激活的受体，这加速了气味受体的识别。对任何气味敏感的受体，当与单个气味受体功能的体外测定相结合时，可以测试有关由气味剂和气味混合物激活的受体组的假设。第一个目标是针对特定区域。在广泛的气味剂领域中，测试对这些气味剂反应的受体组的重叠是否取决于它们的结构相似性。第二个目标是测试有关气味剂受体上气味剂之间可能相互作用的假设。怀疑相互作用，主要是通过一种气味剂阻止另一种气味剂激活一个或多个受体的能力，然后继续测试更复杂的混合物，试图确定受体上气味剂之间的相互作用。 - 对气味的特征感觉和区分一种气味和另一种气味至关重要的影响除了提高我们对气味感知机制的理解之外，这些实验还提供了有望促进更复杂的气味处理方法的发展的知识。环境有利于人类生活质量。