Diagnosis of Osteochondrosis using high field MRI

使用高场 MRI 诊断骨软骨病

• 批准号: 8281914
• 负责人: Cathy S. Carlson
• 金额: $ 14.86万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8281914
• 关键词: 3-Dimensional; Adolescent; Affect; Animal Model; Animals; Area; Barium Sulfate; Biology; Biomechanics; Blood Vessels; Calcified; Cartilage; Cartilage Matrix; Child; Childhood; Chronic; Clinical; Collagen; Collagen Type I; Computer Simulation; Degenerative polyarthritis; Development; Diagnosis; Diagnostic Procedure; Disease; Distal; Early Diagnosis; Equus caballus; Excision; Failure; Family suidae; Femur; Fibrocartilages; Frostbite; Functional disorder; Funding; Goals; Growth; Healed; Human; Hyaline Cartilage; Image; Imaging Techniques; Imaging technology; Immunohistochemistry; Injury; Joints; K-Series Research Career Programs; Knowledge; Lesion; Life Style; Magnetic Resonance; Magnetic Resonance Imaging; Magnetism; Maps; Mentors; Methodology; Methods; Minnesota; Monitor; Necrosis; Operative Surgical Procedures; Orthopedic Surgery procedures; Orthopedics; Osteochondritis Dissecans; Osteochondrosis; Pain; Pathogenesis; Pathologist; Physiologic pulse; Prevalence; Proteoglycan; Qualifying; Recommendation; Replacement Arthroplasty; Request for Applications; Research; Research Activity; Research Personnel; Role; Scientist; Secondary to; Sepsis; Services; Signal Transduction; Sirius Red F3B; Site; Specimen; Staging; Staining method; Stains; Surgical Flaps; Techniques; Time; Tissues; Tolonium chloride; Toxin; Trauma; Universities; Vascular blood supply; Veterinary Medicine; Wages; Water; Work; abstracting; aggrecan; arthropathies; bone; career; cartilage repair; college; disability; experience; healing; humerus; imaging modality; improved; in vivo; innovation; joint injury; knee pain; member; method development; novel; prevent; professor; repaired; technique development; young adult

DESCRIPTION (provided by applicant): Project Summary/Abstract Dr. Cathy Carlson is an experienced, ACVP-boarded veterinary pathologist and orthopaedic researcher who is a tenured full professor in the College of Veterinary Medicine at the University of Minnesota. Although Dr. Carlson is an active research collaborator, gradually increasing service commitments and other responsibilities have prevented her from having a more central role in research in recent years. In this application, she requests 50% salary support for two years while she acquires new knowledge in the area of high field magnetic resonance imaging (MRI) and applies this knowledge to the development of methods to diagnose subclinical lesions of osteochondrosis. For these studies, she will use a well-characterized animal model of osteochondrosis (spontaneous disease in young pigs) with which she has considerable expertise. She will be assisted in these studies by her primary mentor, Dr. Michael Garwood, who is an expert in the area of high field MRI technique development, and her mentoring committee: Dr. Jutta Ellermann (expert in human orthopaedic MRI, including osteochondrosis), Dr. Denis Clohisy (chair of the Department of Orthopaedic Surgery and experienced orthopaedic researcher), and Dr. Jack Lewis (cartilage matrix biology and biomechanics expert). In the proposed, highly innovative studies, the extensive knowledge and experience that Dr. Carlson has with a well-documented animal model of osteochondrosis will be combined with the state-of- the-art high field MRI expertise of the mentor group to identify subclinical lesions of osteochondrosis in intact joints of young pigs, in which the disease occurs with nearly 100% prevalence. Newly developed high field MRI techniques will be used to detect and characterize early damage to the cartilage. The specific aims of the proposed studies are to 1) Develop high-field MRI techniques to identify and characterize subclinical lesions of osteochondrosis, (locally extensive areas of cartilage necrosis), in intact and disarticulated specimens of distal humerus and distal femur (most common predilection sites in both swine and humans) from young pigs; 2) Develop quantitative techniques to characterize the collagen and proteoglycan components of the lesions for comparison with the MRI results; and 3) Develop high-field MRI techniques to identify and map cartilage canal blood vessels and validate these in cleared specimens in which the vessels have been perfused with barium sulfate. This career development award will allow Dr. Carlson the dedicated time to 1) gain expertise in MRI methodology; 2) interact closely with the scientists at the Center for Magnetic Imaging Research; 3) identify and acquire the appropriate porcine specimens for study; 4) assist in interpreting the MRI findings and modifying the techniques through parallel histological studies in the same sites as those evaluated by MRI; and 5) interact with a highly qualified mentor group, the members of which will provide both specific and global input to her proposed research. Dr. Carlson's long-term career goal is to remain in academic research, obtain and maintain RO1 funding for this project, and to devote at least 75% of her effort to research activities. PUBLIC HEALTH RELEVANCE: Project Narrative Osteochondrosis is a focal or multifocal failure in the process of endochondral ossification (conversion of growth cartilage to bone during growth and development) that predisposes the affected site to the formation of a cartilaginous/osteocartilaginous flap (osteochondritis dissecans/OCD), resulting in joint pain and dysfunction. It occurs increasingly commonly in children and young adult patients, and treatment usually includes surgical removal of the flap; however, because of the poor healing qualities of articular cartilage, the affected joint is at risk for the later development of osteoarthritis. If,as proposed in the present application, methods could be developed to diagnose the subclinical disease (prior to the formation of the flap), it is very likely that temporary lifestyle changes, designed to decrease joint trauma, would allow complete and spontaneous healing of the affected site.

描述（由申请人提供）： 项目摘要/摘要 Cathy Carlson 博士是一位经验丰富、获得 ACVP 认证的兽医病理学家和骨科研究员，也是明尼苏达大学兽医学院的终身正教授。尽管卡尔森博士是一位积极的研究合作者，但近年来逐渐增加的服务承诺和其他责任使她无法在研究中发挥更核心的作用。在此申请中，她要求获得两年 50% 的工资支持，同时获得高场磁共振成像 (MRI) 领域的新知识，并将这些知识应用于开发诊断骨软骨病亚临床病变的方法。在这些研究中，她将使用一种特征明确的骨软骨病（幼猪自发性疾病）动物模型，她对此拥有丰富的专业知识。她将在这些研究中得到她的主要导师 Michael Garwood 博士（高场 MRI 技术开发领域的专家）和她的指导委员会：Jutta Ellermann 博士（人体骨科 MRI 专家，包括骨软骨病）的协助。 、Denis Clohisy 博士（骨科外科系主任、经验丰富的骨科研究员）和 Jack Lewis 博士（软骨基质生物学和生物力学专家）。在拟议的高度创新的研究中，卡尔森博士在骨软骨病动物模型方面拥有的丰富知识和经验将与导师小组最先进的高场 MRI 专业知识相结合，以确定亚临床幼猪完整关节的骨软骨病病变，患病率接近 100%。新开发的高场 MRI 技术将用于检测和表征软骨的早期损伤。拟议研究的具体目标是 1) 开发高场 MRI 技术，以识别和表征完整和脱节的肱骨远端和股骨远端标本（最常见的倾向）中骨软骨病的亚临床病变（局部广泛的软骨坏死区域）猪和人类身上的位点）来自幼猪； 2) 开发定量技术来表征病变的胶原蛋白和蛋白聚糖成分，以便与 MRI 结果进行比较； 3) 开发高场 MRI 技术来识别和绘制软骨管血管图，并在血管已灌注硫酸钡的透明样本中进行验证。该职业发展奖将使 Carlson 博士有时间专注于 1) 获得 MRI 方法方面的专业知识； 2）与磁成像研究中心的科学家密切互动； 3) 识别并获取适当的猪标本以供研究； 4) 通过在与 MRI 评估部位相同的部位进行平行组织学研究，协助解释 MRI 结果并修改技术； 5）与高素质的导师小组互动，该小组的成员将为她提出的研究提供具体和全球的投入。卡尔森博士的长期职业目标是继续从事学术研究，获得并维持该项目的RO1资助，并将至少75%的精力投入到研究活动中。 公共健康相关性：项目叙述骨软骨病是软骨内骨化（生长和发育过程中生长软骨转化为骨骼）过程中的局灶性或多灶性失败，使受影响部位容易形成软骨/骨软骨瓣（剥脱性骨软骨炎/强迫症） ），导致关节疼痛和功能障碍。它在儿童和年轻成人患者中越来越常见，治疗通常包括手术切除皮瓣；然而，由于关节软骨的愈合质量较差，受影响的关节有以后发展为骨关节炎的风险。如本申请中所提出的，如果可以开发出诊断亚临床疾病的方法（在皮瓣形成之前），则很可能旨在减少关节创伤的临时生活方式改变将允许完全且自发的愈合。受影响的站点。