ShEEP Proposal for a Multiuser Advanced Biosafe Flow Cytometer

ShEEP 针对多用户高级生物安全流式细胞仪的提案

• 批准号: 9211532
• 负责人: Zhi Q. Yao
• 金额: --
• 依托单位: JAMES H QUILLEN VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9211532
• 关键词: Address; Advisory Committees; B-Lymphocytes; Basic Science; Biological; CD4 Positive T Lymphocytes; CD8B1 gene; Cell Separation; Cells; Chronic; Clinical; Clinical Sciences; Color; Communicable Diseases; Computer software; Contract Services; Core Facility; Data; Fees; Flow Cytometry; Funding; Grant; HIV; HIV/HCV; Health; Hepatitis C virus; Home environment; Housing; Immune; Immune System Diseases; Immunologics; Infection; Lasers; Maintenance; Medical center; Medicine; Minor; Natural Killer Cells; Population; Publishing; Radial; Research; Research Personnel; Safety; Sampling; Scientist; Sorting - Cell Movement; System; T-Lymphocyte; Techniques; Technology; Temperature; Time; Translational Research; Universities; Veterans; abstracting; aged; base; cohort; college; color detection; experience; improved; instrument; macrophage; meetings; monocyte; programs; repository; research study; solid state; transcription factor; translational study; vaccine response

Abstract This request is for a BD FACSAria Fusion flow cytometer which will function as a multiuser instrument in a core facility at the Mountain Home Medical Center. The system will significantly enhance the research of major users who are conducting VA funded research and multiple minor users. While this cytometer will primarily be used for VA funded research, instrument time will also be provided to new investigators and investigators seeking to obtain preliminary data for submission of VA grants. The Mountain Home VA Medical Center currently has no reliable multiuser flow cytometer, and currently utilizing an aged FacsCalibur flow cytometer that was purchased in 2000 and which is owned and operated at the Quillen College of Medicine; while the cytometer continues to be used extensively by the investigators participating in this application, it has limited color options, no sorting capacity, and must be fee-based. All of the VA and University investigators are dependent on this flow cytometer. Cytometric technology has advanced dramatically in the last several years and the research questions that we are currently asking require that we employ state-of-the-art cytometric techniques that are not available on our current instrument. We are clearly at the point where our current cytometer does not meet the needs of VA funded investigators, many of whom require not only sorting capabilities but also an integrated biosafety cabinet to meet the requirements of bioprotection inherent in their translational studies. Furthermore, there are no flow cytometers comparable to the BD FACSAria Fusion, requested in this application, within a 200 mile radius of our VA. Therefore, this application addresses a significant research need. The Mountain Home VA has a strong institutional commitment to the acquisition and maintenance of the flow cytometer. A 500 sq. ft. room, that is appropriately outfitted to house the instrument, has been allocated. Provisions have been made to underwrite the service contract once the initial warranty period has expired. An advisory committee has been established. A doctoral level scientist, with experience in flow cytometry, will supervise the facility with the assistance of an experienced research technician. This instrumental will significantly improve the research efforts at Mountain Home VA aimed at improving veterans' health.

抽象的 此请求是BD Facsaria Fusion流式细胞仪，该仪将充当多源 在山区家庭医疗中心的核心设施中进行仪器。该系统将大大 加强进行VA资助研究和多重研究的主要用户的研究 次要用户。虽然该细胞仪主要用于VA资助的研究，但仪器时间 还将向寻求获取初步数据的新调查人员和调查人员提供 为了提交VA赠款。山地弗吉尼亚州医疗中心目前没有可靠的 多源流式细胞仪，目前使用老化的FACSCALIBUR流式细胞仪 于2000年购买，并在Quillen医学院拥有和经营；尽管 细胞仪继续由参与此的研究人员广泛使用 应用程序，颜色选项有限，无分类容量，并且必须基于收费。全部 VA和大学研究人员取决于该流式细胞仪。细胞仪技术 在过去的几年中，已经显着提高了，我们是我们的研究问题 目前要求我们采用最先进的细胞术技术 在我们当前的乐器上可用。我们显然在当前的细胞仪 不满足VA资助的调查人员的需求，其中许多人不仅需要分类 功能，也是一个集成的生物安全机柜，可满足生物保护要求 他们的翻译研究固有。此外，没有流式细胞仪可与 BD Facsaria Fusion在我们VA的200英里半径内，在本申请中要求。 因此，该应用程序解决了巨大的研究需求。 VA的山区家庭 对流式细胞仪的获取和维护的强烈制度承诺。一个 已经分配了500平方英尺的房间，适合容纳乐器。 一旦初始保修期，已经制定了为服务合同承销服务合同的规定 已经过期了。已经成立了一个咨询委员会。博士学位科学家，有 流式细胞仪的经验将在经验丰富的协助下监督该设施 研究技术员。这种工具将大大改善山区的研究工作 弗吉尼亚州旨在改善退伍军人的健康。