Oklahoma Nathan Shock Center of Excellence in Basic Biology of Aging

俄克拉荷马州内森休克衰老基础生物学卓越中心

• 批准号: 9110089
• 负责人: ARLAN G. RICHARDSON
• 金额: $ 74.85万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9110089
• 关键词: Aging; Animal Model; Animals; Arts; Bioinformatics; Biological Assay; Biology; Biology of Aging; Caenorhabditis elegans Proteins; Cells; Chronic Disease; Cities; Communities; Country; Couples; DNA Methylation; Data; Deletion Mutation; Development; Discipline; Disease; Drosophila genus; Education; Faculty; Foundations; Funding; Funding Agency; Genes; Geroscience; Goals; Grant; Health; Health Sciences; Human; Institutes; Institution; Interest Group; Leadership; Life; Mass Spectrum Analysis; Measurement; Measures; Medical Research; Medical center; Mentors; Methods; Mitochondria; Molecular; Oklahoma; Onset of illness; Organism; Oxidation-Reduction; Oxidative Stress; Pathway interactions; Peptides; Proteins; Research; Research Personnel; Resources; Risk Factors; Role; Running; Sampling; Series; Services; Shock; Speed; Structure; Technology; Time; Tissues; United States National Institutes of Health; Universities; Variant; Yeasts; age related; career; catalyst; design; disorder prevention; experience; genome sequencing; genome-wide; innovation; instrumentation; interest; mass spectrometer; mitochondrial genome; next generation sequencing; non-genetic; novel; oxidative damage; programs; research and development; tool

DESCRIPTION (provided by applicant): The Oklahoma Nathan Shock Center will focus on the newly-developing field of Geroscience and on providing investigators across the nation with unique and innovative assays that are accessible to the entire research community studying aging and Geroscience. The 5 Cores in the Oklahoma Nathan Shock Center are: Program Enhancement Core: In addition, to overseeing all the activities of the Shock Center, the Program Enhancement Core will administer two novel programs. The development of the Oklahoma Geroscience Consortium will bring together investigators interested in aging and Geroscience from the five major research institutions in Oklahoma. In addition, through a Discovery Bioinformatics Component, the Core will provide investigators in aging with unique bioinformatics tools and methods to discover new connections between their data and age-related phenomenon. Research Development Core: This Core is an innovative departure from the traditional structure of similar Cores in Nathan Shock Centers. The Research Development Core of the Oklahoma Nathan Shock Center is designed to provide unprecedented service to the scientific community at large, not just investigators at the host institution. Multiplexing Protein Quantification Core: This Core will provide investigators with a novel mass spectrometry method for measuring the abundance of proteins from any tissue/cell from any organism that has its genome sequenced. The Core can quantify panels of 25 to 30 proteins in a single run, thereby allowing investigators to interrogate pathways of importance in aging and Geroscience. By developing panels of proteins for C elegans, Drosophila, and yeast, as well as exceptionally long-lived animals, this Core will provide investigators, for the first-time, a method for measurin protein abundance in these animal models, which are important to aging. Targeted DNA Methylation & Mitochondrial Heteroplasmy Core: Using novel next-generation sequencing with unique primer sets, which are currently available only in this Core, it will be possible for investigators to study DNA methylation either genome-wide or in specific genes. In addition, the Core has developed methods that will allow investigators to conduct a comprehensive analysis of mitochondrial genome heteroplasmy (variants/mutations and deletions) and copy number from very small samples in a high throughput manner. Integrative Redox Biology Core: This Core will provide investigators with a comprehensive state-of-the-art assessment of the oxidative stress status of a cell, e.g., measures of oxidative damage and redox couples, which require major instrumentation and expertise to obtain accurate and reproducible data. In the past, the Leaders of this Core have experienced a high demand for these assays from investigators across the country.

描述（由申请人提供）：俄克拉荷马州内森冲击中心将重点关注新开发的Geroscience领域，并为全国各地的研究人员提供独特而创新的测定，这些测定能够访问整个研究社区，研究衰老和Geroscience的整个研究社区。俄克拉荷马州内森冲击中心的5个核心是：计划增强核心：此外，为了监督冲击中心的所有活动，计划增强核心将管理两个新颖的程序。俄克拉荷马州埃克拉荷马州的Geroscience联盟的发展将使对俄克拉荷马州五个主要研究机构的老化和Geroscience感兴趣的调查人员聚集在一起。此外，通过发现生物信息学组件，核心将为研究人员提供衰老的独特生物信息学工具和方法，以发现其数据与年龄相关现象之间的新连接。研究开发核心：这种核心与内森冲击中心类似核心的传统结构具有创新性。俄克拉荷马州内森冲击中心的研究开发核心旨在为整个科学界提供前所未有的服务，而不仅仅是东道国的研究人员。多路复用蛋白质定量核心：该核心将为研究者提供一种新型的质谱法，以测量来自任何具有其基因组测序的生物体的组织/细胞中蛋白质的丰度。核心可以在一次运行中量化25至30个蛋白质的面板，从而使研究人员可以询问在衰老和Geroscience中重要的途径。通过开发C秀丽隐杆线虫，果蝇和酵母以及异常长寿动物的蛋白质面板，该核心将为研究人员提供首次研究，这是这些动物模型中测量蛋白丰度的一种方法，这对于对这对于对这对于对这对于对此非常重要的方法，这对于对这对于对此很重要，这对于对此很重要。老化。靶向的DNA甲基化和线粒体异质核心：使用具有独特底漆集的新型下一代测序，目前仅在该核心中可用，研究人员有可能研究全基因组或特定基因的DNA甲基化。此外，核心还开发了方法，可以使研究人员对线粒体基因组异质（变体/突变和缺失）进行全面分析，并以高吞吐量的方式从很小的样本中进行复制数量。综合氧化还原生物学核心：该核心将为研究人员提供对细胞氧化应激状态的全面最新评估，例如，氧化损伤的度量和氧化还原夫妇的测量，这些夫妻需要主要的仪器和专业知识才能获得准确的和专业知识可再现数据。过去，该核心的领导人对全国调查人员的这些测定法的需求很高。