Muscle preservation during weight loss in older, overweight individuals

老年超重人士减肥期间的肌肉保存

• 批准号: 8979655
• 负责人: ROBERT R WOLFE
• 金额: $ 14.67万
• 依托单位: ESSENTIAL BLENDS, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8979655
• 关键词: 3 year old; Acceleration; Activities of Daily Living; Address; Adipose tissue; Age-Years; Aging; Arginine; Bariatrics; Beverages; Biological Preservation; Blood flow; Body Weight; Body Weight decreased; Body mass index; Caloric Restriction; Cities; Citrulline; Clinical; Data; Deposition; Development; Diet; Drug Formulations; Effectiveness; Elderly; Energy Intake; Essential Amino Acids; Focus Groups; Fostering; Goals; Impairment; Incidence; Individual; Ingestion; Intake; Maintenance; Marketing; Measures; Metabolic; Methodology; Methods; Muscle; Muscle Proteins; Muscular Atrophy; Nitric Oxide; Nutrient; Obesity; Outcome Study; Overweight; Phase; Production; Protein Biosynthesis; Proteins; Randomized Clinical Trials; Relative (related person); Research Infrastructure; Resistance; Site; Skeletal Muscle; Taste Perception; Testing; Time; Tracer; Translational Research; Whey Protein; Work; base; cost; cost effective; density; design; food science; lean body mass; meetings; muscle form; nutrition; protein intake; public health relevance; response; sarcopenia; stable isotope; success; technological innovation; volunteer

 DESCRIPTION (provided by applicant): The overall goal of this project is to test a meal replacement beverage that will ultimately preserve muscle loss during calorie restriction weight loss (CRWL) in obese older individuals. We previously developed a meal replacement rich with a proprietary blend of essential amino acids (TMR) that was more than three times as effective as whey protein in stimulating muscle protein synthesis (MPS) in the elderly. Utilizing this TMR and protein intake based on minimal protein requirements (0.8 gm protein/kg/day), we demonstrated a greater reduction in adipose tissue compared to a competitive meal replacement. Even so, volunteers still lost some muscle mass using the TMR. This likely reflected the fact that the optimal protein intake in older individuals is significantly greater thn 0.8 g/kg/day, and the hypocaloric condition of CRWL further elevated the need for optimal protein. In our formulation (MyoEon), we have now increased the absolute amount of EAAs and also included citrulline to even further promote the availability of EAAs to skeletal muscle. Although MyoEon is sufficiently developed for testing the central hypothesis of this project, part of this Phase 1 project will be to further optimize several aspects of product. The overarching hypothesis of this proposal is that the ingestion of MyoEon will foster an improvement in net protein synthesis, through an increased rate of protein synthesis and a decreased rate of protein breakdown as compared to a commercially available "high protein" meal replacement (Bariatric Advantage). Specific Aim 1: We will optimize the palatability and cost for ingredients, production, packaging, and fulfillment, which will validate the hypothesis that MyoEon can be profitably sold in retail markets. We will work directly with our food science partner (Mattson, Inc., Foster City, CA; http://www.foodcom.com/) to establish the criteria of acceptance for the formulation of MyoEon. The metric of success will be defined by establishing the feasibility of MyoEon as a palatable (determined by focus groups) and cost effective (total costs per product less than $1.00) product for use in weight loss. Specific Aim 2: We will demonstrate the feasibility of MyoEon to preserve lean body mass during CRWL as compared to Bariatric Advantage, through increased net protein synthesis, calculated as the difference between protein synthesis and breakdown determined by stable isotope tracer methodology. Demonstration that MyoEon is superior to the highest quality competitor will represent the metric of success for this aim and serve as the mechanistic underpinning for a Phase II randomized, clinical trial (RCT) performed in conjunction with the Mountain West Clinical Translational Research - Infrastructure Network. In this RCT, we will establish the effectiveness of MyoEon vs Bariatric Advantage in an outcome study focused on maintenance of muscle mass and improvement of functional capacity, as well as amplification of the metabolic benefits of weight loss.

 描述（由申请人提供）：该项目的总体目标是测试一种代餐饮料，该饮料最终能够在肥胖老年人的热量限制减肥（CRWL）过程中保持肌肉损失。我们之前开发了一种富含专有混合物的代餐饮料。必需氨基酸 (TMR) 在刺激老年人肌肉蛋白合成 (MPS) 方面的效果是乳清蛋白的三倍以上，并且基于最低蛋白质需求量（0.8 克）利用这种 TMR 和蛋白质摄入量。蛋白质/公斤/天），我们证明，与竞争性代餐相比，志愿者仍然损失了一些肌肉质量，这可能反映了老年人的最佳蛋白质摄入量显着减少的事实。大于 0.8 g/kg/天，并且 CRWL 的低热量条件进一步提高了对最佳蛋白质的需求。在我们的配方 (MyoEon) 中，我们现在增加了 EAA 的绝对量，并且还进一步添加了瓜氨酸。虽然 MyoEon 已充分开发用于测试该项目的中心假设，但该第一阶段项目的一部分将进一步优化产品的几个方面。该提案的总体假设是摄入 EAA。与市售的“高蛋白”代餐相比，MyoEon 将通过提高蛋白质合成率和降低蛋白质分解率来促进净蛋白质合成的改善（减肥优势）。我们将优化原料、生产、包装和履行的适口性和成本，这将验证 MyoEon 可以在零售市场上盈利的假设。我们将直接与我们的食品科学合作伙伴（位于福斯特城的 Mattson, Inc.）合作。 CA；http://www.foodcom.com/）建立 MyoEon 配方的接受标准 成功的衡量标准将通过确定 MyoEon 的可行性（由焦点小组确定）和成本来定义。用于减肥的有效产品（每个产品的总成本低于 1.00 美元） 具体目标 2：我们将证明与减肥优势相比，MyoEon 在 CRWL 期间通过增加净蛋白质合成（计算为通过稳定同位素示踪剂方法确定的蛋白质合成和分解之间的差异证明 MyoEon 优于最高质量的竞争对手将代表这一目标的成功指标，并作为机械基础。与 Mountain West 临床转化研究 - 基础设施网络联合进行的 II 期随机临床试验 (RCT) 在此 RCT 中，我们将在一项侧重于维持肌肉质量和减肥效果的结果研究中确定 MyoEon 与减肥优势的有效性。提高功能能力，以及放大减肥的代谢益处。