EFFECTS OF LEUCINE ENHANCED AMINO ACID DRINK ON MUSCLE PROTEIN METABOLISM IN THE

亮氨酸增强氨基酸饮料对肌肉蛋白质代谢的影响

• 批准号: 7605384
• 负责人: ROBERT R WOLFE
• 金额: $ 1.49万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605384
• 关键词: Activities of Daily Living; Adult; Age; Amino Acids; Biopsy; Cardiovascular system; Computer Retrieval of Information on Scientific Projects Database; Data; Elderly; Essential Amino Acids; Female; Fracture; Funding; Goals; Grant; Hour; Individual; Ingestion; Institution; Investigation; Kinetics; Leucine; Metabolic; Methodology; Muscle; Muscle Proteins; Patients; Peripheral Vascular Diseases; Protein Biosynthesis; Research; Research Personnel; Resources; Risk; Sampling; Skeletal Muscle; Source; Supplementation; Tissues; Tracer; United States National Institutes of Health; Venous; Weight; day; drinking; experience; falls; male; protein metabolism; research study; sarcopenia; stable isotope

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many adults experience a progressive loss of skeletal muscle mass with increasing age. This concomitant loss of contractile tissue and functional capacity (i.e., sarcopenia) contributes to increased risk of falls, bone fracture and may substantially impair many activities of daily living. Given the fact that net muscle protein synthesis is a major metabolic determinant of muscular strength and functional capacity, the goal of this study is to determine if a leucine enhanced amino acid drink will stimulate net muscle protein synthesis to a greater extent compared to an equivalent (same weight in terms of amino acid composition) amino acid drink containing only the essential amino acids. The direct comparison of those two drinks will provide new information on the effects of including a larger amount of leucine (compared to the other essential amino acids) in a drink, which will serve as important preliminary data for subsequent investigations looking at optimizing amino acid supplementation in elderly individuals. To achieve the goals of this research, a total of 8 healthy patients with no prior cardiovascular or peripheral vascular disease will participate in this research study. Subjects will be normal healthy males and females between the ages of 60 and 85. The study will consist of two 8-hour trials performed on separate days. The study will examine muscle protein kinetics prior to and following the ingestion of the amino acids containing drinks. Muscle protein kinetics will be determined using established stable isotope tracer methodology, arterial-venous sampling and muscle biopsies.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 随着年龄的增长，许多成年人经历了骨骼肌质量的逐渐丧失。这种伴随的收缩组织损失和功能能力（即肌肉减少症）有助于增加跌倒，骨折的风险，并可能严重损害许多日常生活的活动。 鉴于净肌肉蛋白合成是肌肉强度和功能能力的主要代谢决定因素，这项研究的目的是确定亮氨酸增强的氨基酸饮料是否会刺激净肌肉蛋白合成，而与氨基酸组成相比，仅包含氨基酸含量的氨基酸含量相同的氨基酸含量相同。这两种饮料的直接比较将提供有关在饮料中包括大量亮氨酸（与其他必需氨基酸相比）的效果的新信息，该饮料将作为重要的初步数据，以进行随后的研究，以期在老年人中优化补充氨基酸。为了实现这项研究的目标，总共8例没有先前没有心血管或周围血管疾病的健康患者将参加这项研究。受试者将是正常的健康男性和60至85岁之间的女性。该研究将包括在不同的日子进行的两次8小时试验。该研究将在摄入含有饮料的氨基酸之前和之后检查肌肉蛋白动力学。肌肉蛋白动力学将使用已建立的稳定同位素示踪剂方法，动脉 - 观测性采样和肌肉活检确定。