Quantitative proteomics of Xenopus oocytes, eggs, blastomeres, and nuclear transplants

非洲爪蟾卵母细胞、卵子、卵裂球和核移植物的定量蛋白质组学

基本信息

批准号：
9116239
负责人：
Norman J Dovichi
金额：
$ 31.22万
依托单位：
UNIVERSITY OF NOTRE DAME
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-01 至 2020-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9116239
关键词：
Address Animals Antibodies Brain Cell Cycle Cell Lineage Cells Chromatin Chromatin Structure Communities Complex Cryoultramicrotomy Data Databases Development Embryo Embryonic Development Event Evolution Fertilization Future Genome Genomics Goals Health Kinetics Label Measures Modeling Molecular Monitor Nuclear Oocytes Pathway interactions Pattern Pattern Formation Phosphorylation Phosphorylation Site Post-Translational Protein Processing Process Protein Dynamics Proteins Proteome Proteomics Publishing Regenerative Medicine Reporting Research Resources Retina Signal Transduction Slice Spinal Cord Staging Stem cells Technology Tissues Tracer Transplantation Work Xenopus Xenopus laevis Xenopus oocyte abstracting egg embryo cell embryo stage 2 improved insight morphogens multiple reaction monitoring nuclear reprogramming nuclear transfer oocyte maturation pluripotency protein expression relating to nervous system research study tandem mass spectrometry tool web site xenopus development zygote

项目摘要

DESCRIPTION (provided by applicant): Abstract. This research team recently published the first quantitative proteomic analysis of a devel- oping embryo (Sci Rep. 2014; 4: 4365. PMID: 24626130). We studied single Xenopus laevis embryos at six stages of development. We initially quantified the expression change of 4,000 proteins across at least four stages of development; reanalysis of the data with an improved genomic database has extended our identifications by 50%. Data have been made available to the community on the Scien- tific Reports web site, at Xenbase, and at PeptideAtlas. We will extend our initial study by quantitatively monitoring the expression and phosphorylation status of proteins isolated from single Xenopus oocytes, eggs, and blastomeres. Quantitative protein expression changes will also be measured from cryosections perpendicular to developmental axes to identify patterning gradients, along a selected cell lineage, from an explant of that lineage, and in oocytes following nuclear transplantation. The results of these studies will be an unprecedented resource to the Xenopus community by providing detailed data on the evolution of protein expression during development, and chromatin reorganization in embryos and following nuclear transplantation. We will work with Xenbase, PeptideAtlas, and VectorBase to disseminate our results as widely as possible.

描述（由申请人提供）：抽象的。该研究小组最近发布了开发胚胎的首次定量蛋白质组学分析（SciRep。2014; 4：4365。PMID：24626130）。我们在开发的六个阶段研究了单爪蟾laevis胚胎。我们最初在至少四个发育阶段中量化了4,000种蛋白质的表达变化；用改进的基因组数据库重新分析数据已将我们的识别量扩大了50％。数据已在Scien-Tific Reports网站，XENBASE和PEPTIDEATLAS上提供给社区。我们将通过定量监测从单爪蟾卵母细胞，卵和胚泡分离的蛋白质的表达和磷酸化状态来扩展我们的初步研究。定量蛋白表达的变化也将从垂直于开发轴的冷冻切除术来测量，以识别沿所选细胞谱系的模式梯度，从该谱系的外观以及核移植后的卵母细胞中。这些研究的结果将通过提供有关发育过程中蛋白质表达的演化的详细数据以及胚胎中的染色质重组和核移植后的染色质重组，这将是对爪蟾社区的前所未有的资源。我们将与Xenbase，PeptideAtlas和VectorBase合作，尽可能广泛地传播我们的结果。