Upgrade for a 600 MHz Structural Biology NMR

升级为 600 MHz 结构生物学 NMR

• 批准号: 9075568
• 负责人: JAMES H. PRESTEGARD
• 金额: $ 59.99万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075568
• 关键词: 17 year old; Adopted; Area; Carbon; Communities; Complex; Detection; Development; Faculty; Future; Grant; Heat shock proteins; Immune response; Metabolism; Minor; NMR Spectroscopy; Natural Products; Neoplasm Metastasis; Performance; Polysaccharides; Post-Translational Protein Processing; Proteins; Protocols documentation; Protons; Regulation; Research; Role; Scientist; Signal Transduction; Site; Structure; System; United States National Institutes of Health; Universities; Virulence Factors; Work; base; digital; drug efficacy; improved; instrument; instrumentation; pathogenic bacteria; programs; protein complex; research study; response; stress protein; structural biology

 DESCRIPTION (provided by applicant): An upgrade of an existing 600 MHz NMR spectrometer used primarily for structural biology applications is proposed. The upgrade will replace a 17 year old Varian INOVA console with a new Bruker Avance III HD 600 High Performance Digital NMR Console and an existing cryobay with a Bruker cryoplatform. The upgrade will allow the use of a high sensitivity cryoprobe that is optimized for 13C (and 15N) detection along with 1H and 2H decoupling. The instrument is being placed in a well-staffed and supported NMR facility that serves the University of Georgia (UGA) scientific community, as well as a number of additional scientists throughout the region. The ability to observe 13C directly with high sensitivity will add to the facility a capability that is unique, and one that is becomin increasingly important in the characterization of biomolecular systems. These systems include large proteins or proteins that are parts of large complexes, proteins with intrinsically disordere regions, and natural products (including some glycans) that have significant numbers of non- protonated carbon sites. In addition to this unique capability, the system will execute, with sensitivity comparable or greater than non-cryo systems, the traditional proton detected triple resonance experiments that have become so important to structure determination of biomedically important proteins. In the latter case the more reliable and up-to-date system will add important capacity to serve the growing number of NMR users who pursue NMR-based structural work at UGA. Seven major users and three minor users have provided research descriptions as a part of this proposal. Their projects impact on areas such as: the role of glycans in development and metastasis, protein modifications in response to environmental stress, protein interactions in cell signaling and immune response, regulation of virulence factors in pathogenic bacteria, and improving drug efficacy through regulation of transport and metabolism. Seven of the ten users are currently supported by NIH grants, and we expect additional NIH support as the newly added faculty establish their UGA research programs. The requested instrumentation will allow them that access to NMR instrumentation needed for their current research, and it will allow them to adopt new experimental protocols that allow them to attack ever more complex problems in the future.

 描述（由应用程序提供）：提出了主要用于结构生物学应用的现有600 MHz NMR光谱仪的升级。升级将用新的Bruker Avance III HD 600高性能数字NMR控制台和带有Bruker Cryoplatform的现有Crobay替换17岁的Varian Inova控制台。该升级将允许使用高灵敏度的冷冻探针，该冷冻探针可针对13C（和15N）检测以及1H和2H解耦进行优化。该工具被安置在服务良好且支持的NMR设施中，该设施为佐治亚大学（UGA）科学界以及整个地区的许多其他科学家提供服务。直接以高灵敏度观察13C的能力将增加独特的功能，并且在生物分子系统的表征中变得越来越重要。这些系统包括大型蛋白质或蛋白质，这些蛋白质是大型复合物的一部分，具有内在疾病区域的蛋白质以及天然产物（包括一些聚糖），这些蛋白质具有大量非蛋白酶位的碳位点。除了这种独特的能力外，该系统还将执行，敏感性可比性或大于非晶体系统，传统的质子检测到的三谐振实验对于结构生物医学重要的蛋白质的确定非常重要。在后来的情况下，更可靠和最新的系统将增加重要的能力，以服务于越来越多的NMR用户在UGA中从事基于NMR的结构工作。七个主要用户和三个未成年人提供了研究描述，作为本提案的一部分。它们的项目对以下区域的影响：聚糖在发育和转移中的作用，响应环境压力的蛋白质修饰，细胞信号中的蛋白质相互作用和免疫反应，调节病毒因子。 在致病细菌中，通过调节转运和代谢来提高药物效率。十个用户中有七个目前得到NIH赠款的支持，我们希望随着新添加的教职员工建立他们的UGA研究计划，我们预计NIH的支持。要求的仪器将使他们能够访问其当前研究所需的NMR仪器，并且它将使他们能够采用新的实验协议，使他们将来可以攻击越来越复杂的问题。

项目成果

Dissolved organic metabolite extraction from high‐salt media

从高盐介质中提取溶解的有机代谢物

• DOI: 10.1002/nbm.4797
• 发表时间: 2022
• 期刊: NMR in Biomedicine
• 影响因子: 2.9
• 作者: Holderman, Nicole R.;Ferrer‐González, Frank X.;Glushka, John;Moran, Mary Ann;Edison, Arthur S.
• 通讯作者: Edison, Arthur S.