Traditional Service Core [Translational Service Core (TSC)]

传统服务核心【翻译服务核心（TSC）】

• 批准号: 8882368
• 负责人: JENNIFER M LOFTIS
• 金额: $ 21.16万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8882368
• 关键词: Address; Adult; Anxiety; Biological; Biological Assay; Blood; Blood specimen; Clinic; Clinical; Clinical Data; Cognition; Cognitive; Collaborations; Collection; Communities; Community Outreach; Data; Data Collection; Detection; Disease remission; Eligibility Determination; Ensure; Enzyme-Linked Immunosorbent Assay; FDA approved; Future; Human; Immune; Impulsivity; Interview; Investigation; Laboratories; Link; Measures; Medical; Methamphetamine dependence; Methods; Mus; Neuropsychological Tests; Outcome; Participant; Peripheral Blood Mononuclear Cell; Pharmaceutical Preparations; Pilot Projects; Plasma; Procedures; Process; Protocols documentation; Questionnaires; Recording of previous events; Research; Research Personnel; Rodent; Running; Sampling; Services; Site; Specimen; Standardization; Structure; Substance Use Disorder; Symptoms; Testing; Tissue Sample; Tissues; Visit; addiction; base; bench to bedside; biopsychosocial; computerized data processing; dependence relapse; eligible participant; enzyme linked immunospot assay; human data; human subject; human tissue; improved; methamphetamine abuse; methamphetamine use; neuroadaptation; neuroimaging; neuroinflammation; neuropsychiatry; programs; repository; research study; response; screening; success; therapy development; treatment center; treatment program

To ensure the success of the overall research program, and rapid translational progress from the bench to the bedside, we propose in the current application the establishment of a Translational Service Core (TSC). The primary objective of the TSC is to standardize the Methamphetamine Abuse Research Center's (MARC's) procedures for collecting human neuropsychiatric data, processing and storing human blood, and measuring immune factors and responses in human and rodent samples. A secondary objective is to establish a repository for human neuropsychiatric data and blood samples. These de-identified samples, linked to the neuropsychiatric data, will be available for use in future studies, and therefore have significant long-term value to the MARC. All three proposed Scientific Components (Components 7, 8, & 9) analyze human or mouse tissue to evaluate the relationship between immune factors and neuropsychiatric outcomes (e.g., cognition, anxiety). Two of the three proposed Scientific Components (Components 7 & 8) analyze neuropsychiatric data from human subjects. These Components and the MARC would benefit from a centralized TSC to eliminate redundant recruitment efforts, standardize operating procedures, and improve cross-component and translational analyses. The TSC will recuit 300 subjects into three groups (100/group): 1) adults actively using methamphetamine (MA), 2) adults in early remission from MA dependence, and 3) non-dependent adults. TSC staff will run subjects through a neuropsychiatric study visit including: 1) a clinical interview to collect biopsychosocial history, 2) a structured clinical interview to verify psychiatric and substance use disorders, 3) psychiatric symptom questionnaires, 4) neuropsychological tests covering a full range of cognitive domains, 5) medical laboratory tests, and 6) a blood draw for collection of plasma and peripheral blood mononuclear cells. Samples will be stored in the TSC repository and transferred to Components and Pilot Projects in service of their objectives. The TSC staff will also guide analyses of human and rodent tissue for immune factor expression using standardized methods (e.g., ELISA, Luminex, ELISPOT, and FACS), thus increasing efficiency and collaboration among Components.

为了确保整体研究计划的成功以及从长凳到床边的快速翻译进展，我们在当前应用中提出了转化服务核心（TSC）的建立。 TSC的主要目的是标准化甲基苯丙胺滥用研究中心（MARC）的程序，以收集人类神经精神数据，加工和储存人体血液，并测量人类和啮齿动物样本中的免疫因素和反应。次要目标是建立一个人类神经精神数据和血液样本的存储库。这些与神经精神病数据相关的取消识别样本将用于未来的研究，因此对MARC具有显着的长期价值。所有三个提出的科学成分（成分7、8和9）均分析人或小鼠组织，以评估免疫因子与神经精神疾病结果之间的关系（例如认知，焦虑）。提出的三个科学组成部分（组件7和8）中有两个分析了人类受试者的神经精神病数据。这些组件和MARC将从集中的TSC中受益，以消除冗余招聘工作，标准化操作程序并改善跨组件和翻译分析。 TSC将使300名受试者分为三组（100/组）：1）成年人使用甲基苯丙胺（MA），2）成年人在MA依赖性中早期缓解，而3）非依赖性成年人。 TSC工作人员将通过神经精神病学研究访问受试者，包括：1）临床访谈，以收集生物心理社会史，2）验证精神病和药物使用障碍的结构化临床访谈，3）精神病症状问卷，4）4）神经心理学测试，涵盖了范围的全部范围，并涵盖了complotip for for Medical draplip and Medical drapters and a flool and Medical ai ai forlip and Medical ai ai graperal and ai a iplair and Medical ai ai a a and a ipsma and a a。血液单核细胞。样品将存储在TSC存储库中，并将其转移到组件和试点项目中，以服务其目标。 TSC工作人员还将使用标准化方法（例如ELISA，Luminex，Elispot和FACS）指导人和啮齿动物组织的分析，以提高组件之间的效率和协作。