Monitoring Pesticide Exposure and Accumulation: An improved Rapid Identifier for all Pesticide Classes

监测农药暴露和积累：针对所有农药类别的改进快速识别器

• 批准号: 8903333
• 负责人: KEVIN M SPENCER
• 金额: $ 14.99万
• 依托单位: EIC LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-15 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8903333
• 关键词: 4-nitrophenol; Adsorption; Amines; Atrazine; Automation; Azides; Biological; Biology; Body Fluids; Calibration; Camping; Chemicals; Chlorinated Hydrocarbons; Communities; Complex; Coupled; Data; Data Set; Databases; Detection; Diesel Fuels; Electrons; Elements; Environmental Monitoring; Environmental Pollution; Epidemiologic Studies; Exposure to; Film; Food Supply; Goals; Health; Human; Humidity; Hydrogen Bonding; Ingestion; Laboratories; Least-Squares Analysis; Life; Liquid substance; Measures; Methods; Methyl Parathion; Monitor; National Institute of Environmental Health Sciences; Organophosphates; Outcome; Pesticide Residues; Pesticides; Phase; Population; Populations at Risk; Process; Protocols documentation; Raman Spectrum Analysis; Reading; Reporting; Reproducibility; Research; Resolution; Sampling; Signal Transduction; Silver; Simazine; Small Business Innovation Research Grant; Solutions; Source; Spectrum Analysis; Statistical Algorithm; Sunlight; Surface; Techniques; Technology; Temperature; Testing; Time; Triazines; United States National Institutes of Health; Urinalysis; Urine; Work; cost; cost effective; design; electron density; farm worker; high risk; improved; innovation; interest; novel; organochlorine pesticide; pesticide exposure; planetary Atmosphere; programs; public health relevance; sensor; surface coating; tool; ultraviolet irradiation; vapor

 DESCRIPTION (provided by applicant): An inexpensive disposable vapor sensor, which can also be used for urinalysis, can provide rapid field monitoring of pesticide exposure. The sensor utilizes Surface-Enhanced Raman Spectroscopy (SERS) to detect chemicals, like pesticides, that adsorb strongly on roughened SERS substrates in the high parts-pre- trillion range. As the sensors are tuned to the analytes of interest, interferences from more concentrated chemicals are limited. An earlier research program provided reliable results for organophosphate (OP) and organochlorine (OC) pesticides. In that program, 50 OP and OC pesticides or metabolites were evaluated in the laboratory at the low ppb range, with acephate and methyl parathion detection at farmworker camps. In this program, we will fabricate an innovative SERS sensing element for the detection of triazine pesticides, such as atrazine, simazine and cyromazine. Sensors will be optimized by an azide loading to improve the electron density and yield strong hydrogen bonding with the triazine amine groups. This novel sensor is expected to detect triazines in concentrations of 1 ppb or less and withstand the temperature and humidity conditions encountered by farmworkers. The new sensor can be easily combined with our previous sensor to measure >75 pesticides or metabolites at a fraction of the cost of current analytical laboratory methods, thus providing the NIH (particularly the NIEHS Exposure Biology program) with a large data set on the daily exposure/ingestion of pesticides. This data will allow epidemiological studies and predictions of long-term health outcomes. Core technology concepts have been demonstrated. A previous program demonstrated organochlorine and organophosphate pesticides can be detected down as low as 100 ppt. EIC Laboratories has demonstrated in preliminary studies that an azide-coated SERS sensor is several orders of magnitude more sensitive to triazines than previous sensors. The Phase I program is designed to demonstrate detection of triazine pesticides and metabolites at the 1 ppb level, show an extended field lifetime and an increased sensitivity for direct readings of triazine pesticide metabolites.

 描述（由适用提供）：一种廉价的一次性蒸气传感器，也可以用于尿液分析，可以提供对农药暴露的快速现场监测。传感器利用表面增强的拉曼光谱法（SERS）来检测农药等化学物质，这些化学物质强烈吸附在高份量范围内粗糙的SERS底物上。随着传感器的调整为感兴趣的分析物，浓缩化学品的访谈受到限制。较早的研究计划为有机磷酸盐（OP）和有机氯（OC）农药提供了可靠的结果。在该计划中，在PPB范围低的实验室中评估了50种OP和OC农药或代谢物，在农场工作人员营地进行了宽松和甲基parathion检测。在该程序中，我们将制造一种创新的SERS感应元件，用于检测三嗪农药，例如抗乙嗪，西米嗪和棒状球菌。传感器将通过叠氮化物负载优化，以提高电子密度并与三嗪胺基团产生强氢键。预计该新型传感器将检测到1 ppb或更少的浓度，并承受农场工人遇到的温度和湿度条件。新的传感器可以轻松与我们以前的传感器相结合，以> 75种农药或代谢物的成本，占当前分析实验室成本的一小部分 方法，从而为NIH（部分是NIEHS暴露生物学计划）提供了有关每日暴露/摄入农药的大数据集。这些数据将允许流行病学研究和长期健康结果的预测。已经证明了核心技术概念。以前的程序表明，可以在低至100 ppt的情况下检测到有机氯和有机磷酸盐农药。 EIC实验室在初步研究中表明，叠叠式SERS传感器比以前的传感器更敏感地对三嗪更敏感。 I阶段计划旨在证明在1 ppb水平的三嗪农药和代谢产物的检测，显示出延长的田间寿命，并且对直接读取​​三嗪农药代谢产物的直接读数的灵敏度提高。