Animal Core

动物核心

基本信息

批准号：
8436391
负责人：
MICHAEL J. FORSTER
金额：
$ 25.7万
依托单位：
UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-15 至 2017-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8436391
关键词：
Activities of Daily Living Address Adult Affective Animal Husbandry Animals Brain Calcium Channel Cognitive Data Databases Diet Environment Equilibrium Equipment and supply inventories Estrogen Receptors Estrogens Experimental Models Female Functional disorder Generations Hormonal Hormone Receptor Hormones Human Implant Individual Injury Instruction Intervention Laboratories Learning Measures Memory Middle Cerebral Artery Occlusion Modeling Molecular Target Monitor Motor Activity Mus Neonatal Neurological status Neurons Operative Surgical Procedures Outcome Assessment Ovariectomy Performance Phase Preparation Procedures Progesterone Progesterone Receptors Psychomotor Performance Rattus Regimen Research Research Personnel Resources Rodent Silastic Simulate Sprague-Dawley Rats Stroke Testing Therapeutic Time Tissues Transgenic Mice Treatment Protocols base behavior test brain tissue cognitive function design diarylpropionitrile functional outcomes functional status hormone therapy implantation instrumentation mimetics morris water maze neonate novel pregnant programs quality assurance reproductive research study senescence steroid hormone young adult

项目摘要

PROJECT SUMMARY (See instructions): The over-arching aim of Core B is to provide to each Project, access to tissue and results relevant to the functional status of ovariectomized rats maintained in two experiments conducted in a standardized setting. In phases I and 11, five treatment regimens [vehicle, estrogen, diarylpropionitrile (DPN), progesterone, or estrogen + progesterone], implemented after 3 different post-ovariectomy delays, will be evaluated for their ability to reverse the functional deficits associated with OVX in young adult (4-month old) and after one delay in reproductive senescent (10 month old) rats. In a subsequent experiment (phase 111), the neuroprotective efficacy of the same treatment regimens will be assessed in the transient middle cerebral artery occlusion (tMCAO) model of stroke. The assessment of functional outcomes at the different post-ovariectomy delays will provide the critical data that allows the individual projects to relate changes in the functions of estrogen receptors, progesterone receptors and intracellular calcium channels, to specific periods of sensitivity or refractoriness to hormone receptor-targeted interventions. Core B will provide standardized implementation of the experimental variables; outcome assessments, environment, and animal husbandry needed for the project experiments, and provide for highly efficient leveraging of resources used by all projects, including staff and animals. To this end. Core B will procure, identify and monitor rats used in the experiments, perform all surgical interventions (ovariectomy, implantation of Silastic pellets and tMCAO), and perform comprehensive analyses of the functional status of the rats in the context of the different treatment regimens. Core B will in addition provide non-behaviorally characterized rats that will have been ovariectomized and exposed to the 5 treatment to Project 2 for synaptoneurosomal fraction preparation, and will maintain availability of neonatal brain tissue for the generation of primary neuronal cultures.

项目摘要（参见说明）：核心 B 的总体目标是为每个项目提供与在标准化环境中进行的两项实验中维持的卵巢切除大鼠功能状态相关的组织和结果。在 I 期和 11 期中，将评估在 3 次不同的卵巢切除术后延迟后实施的五种治疗方案（载体、雌激素、二芳基丙腈 (DPN)、黄体酮或雌激素 + 黄体酮）逆转 OVX 相关功能缺陷的能力在年轻成年（4 个月大）大鼠中和在生殖衰老（10 个月大）大鼠中一次延迟后。在随后的实验（第111期）中，将在短暂性大脑中动脉闭塞（tMCAO）中风模型中评估相同治疗方案的神经保护功效。对不同卵巢切除术后延迟的功能结果的评估将提供关键数据，使各个项目能够将雌激素受体、孕激素受体和细胞内钙通道的功能变化与激素受体敏感或不应期的特定时期联系起来。有针对性的干预措施。 Core B将提供实验变量的标准化实现；项目实验所需的成果评估、环境和畜牧业，并提供对所有项目使用的资源（包括工作人员和动物）的高效利用。为此。 Core B 将采购、识别和监测实验中使用的大鼠，进行所有手术干预（卵巢切除、硅橡胶颗粒植入和 tMCAO），并对不同治疗方案下大鼠的功能状态进行全面分析。核心B还将提供非行为特征的大鼠，这些大鼠将被切除卵巢并接受项目2的5种治疗以制备突触神经体部分，并将维持新生儿脑组织的可用性以产生原代神经元培养物。