Genital Mucosal Immunity in Adolescent Women

青春期女性的生殖器粘膜免疫

基本信息

批准号：
8600649
负责人：
REBECCA Pellett MADAN
金额：
$ 13.36万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-01-15 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8600649
关键词：
21 year old AIDS prevention Address Adolescence Adolescent Adult Affect Age Age Distribution Antiviral Agents Bacteria Biological Biological Assay Biological Markers Biological Response Modifiers CD4 Positive T Lymphocytes Cell Count Cell physiology Cervical Data Defensins Development Plans Disease Ecosystem Environment Epidemiologic Studies Evaluation Evolution Exhibits Female Female Adolescents Foundations Future Genital system Goals HIV HIV Infections Health High Risk Woman Host Defense Human Papillomavirus Hydrogen Peroxide Immune Immune response Immunoglobulins Immunologics In Vitro Infection Infection prevention Intervention Knowledge Laboratories Lactobacillus Lead Liquid substance Mediator of activation protein Menarche Menstruation Mentorship Mucosal Immunity Mucous Membrane Muramidase Mus Natural Immunity Ovulation Peptides Plasma Population Prevention Protein Array Recurrence Research Methodology Research Personnel Risk Safety Sampling Sex Behavior Sexually Transmitted Diseases Simplexvirus Swab Techniques Testing Training Translational Research Vaccines Vagina Viral Load result Woman antimicrobial antiretroviral therapy base career development cellulose sulfate cervicovaginal chemokine cohort cytokine experience genital secretion high risk hypothalamic pituitary ovarian axis meetings microbicide mucosal vaccine novel pathogen pathogenic bacteria prevent reproductive hormone response restoration sexual debut therapy development transmission process trend vaginal microbicide young woman

项目摘要

DESCRIPTION (provided by applicant): Adolescent and young women are at high risk for HIV and STI acquisition. Developing novel, safe, preventative options for this population, such as vaginal microbicides, requires a thorough understanding of mucosal immunity in the female genital tract. Prior studies have evaluated the genital mucosal immune environment in adult women and indicate that protective factors in cervicovaginal fluid exhibit intrinsic activity against HIV, herpes simplex virus, and multiple types of pathogenic bacteria. However these data are lacking in young, adolescent women, and it remains unknown how innate immunity in the genital tract develops during adolescence. Moreover little is known of the functional components of genital mucosal immunity in young women infected with HIV, who may use microbicides with no knowledge of their infection or who may wish to use a microbicide to prevent infection with another STI or to prevent HIV transmission to their partner. This application addresses these knowledge gaps through the following specific aims: [(1) evaluate the differences in genital mucosal immunity in HIV uninfected females ages 13-21 years who are either within three years of menarche or greater than three years from menarche by comparing endogenous antimicrobial activity in genital secretions between the two groups and correlating this activity with specific immune mediators and vaginal microbiota populations; (2) evaluate the differences in genital mucosal immunity between a cohort of HIV infected young women ages 13-21 years and a cohort of healthy, uninfected women of similar age distribution by comparing endogenous antimicrobial activity in genital secretions and correlating this activity with specific immune mediators and vaginal microbiota populations.] The results of these studies will have significant implications for future studies of HIV and STI prevention and acquisition in young women. My objective is to become an independent investigator with expertise in STI acquisition and prevention in adolescent women. I have proposed a career development plan that incorporates mentorship in translational research methods, specialized training in novel laboratory techniques, individualized coursework, and participation in local and national meetings.

描述（由申请人提供）：青少年和年轻妇女遭受艾滋病毒和性传播感染的高风险。为该人群（例如阴道杀菌剂）开发新颖，安全，预防性选择，需要对女性生殖道中的粘膜免疫进行透彻的了解。先前的研究已经评估了成年女性的生殖器粘膜免疫环境，并表明宫颈阴道液中的保护因子表现出针对HIV，单纯疱疹病毒和多种类型的致病细菌的固有活性。然而，这些数据缺乏年轻的青春期女性，并且在青春期期间生殖道中的先天免疫如何发展。此外，对感染艾滋病毒的年轻妇女的生殖器粘膜免疫的功能成分知之甚少，艾滋病毒感染了艾滋病毒，她们可能不了解其感染的菌心，或者希望使用杀菌剂来防止其他性病感染或防止艾滋病毒传播。该申请通过以下特定目的解决这些知识差距：[（1）评估13-21岁的艾滋病毒未感染的女性生殖器粘膜免疫的差异，这些女性在三年内或三年内与初级成年结构的三年内大于三年以上，通过比较内源性抗菌抗菌活性的菌群中的菌群和疾病的菌群中的特定型媒介物以及特定的特定型物种的疾病，（2）评估一组13-21岁的艾滋病毒感染者之间的生殖器粘膜免疫力差异，以及通过比较内源性抗生素分泌的抗菌活性，在生殖器分泌物中比较具有相似年龄分布的健康，未感染的妇女，并将这种活性与特定的免疫媒介和阴道菌群相关联。年轻女性的收购。我的目标是成为一名独立研究者，在青少年妇女中获得性传播感染和预防方面具有专业知识。我提出了一项职业发展计划，该计划纳入了翻译研究方法的指导，新颖的实验室技术专业培训，个性化的课程工作以及参加本地和国家会议。